人类, 小鼠, 大鼠
免疫印迹, 免疫细胞化学, 流式细胞仪, 免疫组化-石蜡切片
图像 1 :
Anti-POR antibody, PA1952, Western blotting. Lane 1: Rat Ovary Tissue Lysate. Lane 2: Rat Lung Tissue Lysate. Lane 3: Rat Testis Tissue Lysate. Lane 4: Rat Spleen Tissue Lysate. Lane 5: A549 Cell Lysate . Lane 6: HELA Cell Lysate. Lane 7: SKOV Cell Lysate. Lane 8: MCF-7 Cell Lysate
图像 2 :
Anti-POR antibody, PA1952, IHC(P). IHC(P): Human Placenta Tissue
图像 3 :
Anti-POR antibody, PA1952, ICC. ICC: HELA Cell
人类, 小鼠, 大鼠
流式细胞仪, 免疫组化-P, 免疫细胞化学, 免疫印迹
Immunohistochemistry (Paraffin-embedded Section), 0.5-1µg/ml, Human, Mouse, Rat, By Heat. Immunocytochemistry, 0.5-1µg/ml, Human Western blot, 0.1-0.5µg/ml, Human, Mouse, Rat.Flow Cytometry, 1-3μg/1x10^6 cells, Human.
Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.
Boster Bio Anti-Cytochrome P450 Reductase/POR Antibody catalog # PA1952. Tested in Flow Cytometry, IHC-P, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
A synthetic peptide corresponding to a sequence at the C-terminus of human POR (659-677aa DYIKKLMTKGRYSLDVWS), different from the related rat and mouse sequences by one amino acid.
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
No cross reactivity with other proteins
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Protein Function :
This enzyme is required for electron transfer from NADP to cytochrome P450 in microsomes. It can also provide electron transfer to heme oxygenase and cytochrome B5.
Subcellular Localization :
Endoplasmic reticulum membrane; Peripheral membrane protein. Anchored to the ER membrane by its N- terminal hydrophobic region.
Recommended Detection Systems :
Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P) and ICC.
Sequence Similarities :
In the C-terminal section; belongs to the flavoprotein pyridine nucleotide cytochrome reductase family.
POR is a membrane-boundenzyme required for electron transfer from NADPH to cytochrome P450 in the endoplasmic reticulum of theeukaryotic cell. The gene encodes an endoplasmic reticulum membrane oxidoreductase with an FAD-binding domain and a flavodoxin-like domain. The protein binds two cofactors, FAD and FMN, which allow it to donate electrons directly from NADPH to all microsomal P450 enzymes. Mutations in this gene have been associated with various diseases, including apparent combined P450C17 and P450C21 deficiency, amenorrhea and disordered steroidogenesis, congenital adrenal hyperplasia and Antley-Bixler syndrome.
Cancer, Metabolism, Mitochondria, Mitochondrial, Mitochondrial Markers, Mitochondrial Metabolism, Organelles, Pathways And Processes, Signal Transduction, Subcellular Markers
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