抗体

抗紧密连接蛋白ZO-2 TJP2抗体

PA1957

100μg/vial

315美元

多克隆

domestic rabbit

未共轭

免疫印迹, 免疫细胞化学, 流式细胞仪, 免疫组化-石蜡切片, 免疫组化-冰冻切片

抗紧密连接蛋白ZO-2 TJP2抗体

315美元

100μg/vial

多克隆

兔

人类

流式细胞仪, IF, 免疫组化-P, 免疫组化-F, 免疫细胞化学, 免疫印迹

Western blot, 0.1-0.5µg/ml, Human. Immunohistochemistry (Paraffin-embedded Section), 0.5-1µg/ml, Human, By Heat. Immunohistochemistry (Frozen Section), 0.5-1µg/ml, Human. Immunocytochemistry/Immunofluorescence, 5µg/ml, Human. Flow Cytometry, 1-3μg/1x10^6 cells, Human.

WB: The detection limit for TJP2 is approximately 0.5ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Boster Bio Anti-Tight junction protein ZO-2 TJP2 Antibody catalog # PA1957. Tested in Flow Cytometry, IF, IHC-P, IHC-F, ICC, WB applications. This antibody reacts with Human.

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

TJP2

Q9UDY2

A synthetic peptide corresponding to a sequence at the C-terminus of human TJP2 (1171-1190aa SEHSKRGYYGQSARYRDTEL).

冻干

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

免疫原亲和纯化

No cross reactivity with other proteins

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

紧密连接蛋白ZO-2

Tight junction protein ZO-2; Tight junction protein 2; Zona occludens protein 2; Zonula occludens protein 2; TJP2; X104, ZO2;

紧密连接蛋白ZO-2

133958分子量

播放a作用在紧密连接和粘着连接

Cell junction, adherens junction. Cell membrane ; Peripheral membrane protein ; Cytoplasmic side. Cell junction, tight junction. Nucleus. Also nuclear under environmental stress conditions and in migratory endothelial cells and subconfluent epithelial cell cultures.

This protein is found in epithelial cell junctions. Isoform A1 is abundant in the heart and brain. Detected in brain and skeletal muscle. It is present almost exclusively in normal tissues. Isoform C1 is expressed at high level in the kidney, pancreas, heart and placenta. Not detected in brain and skeletal muscle. Found in normal as well as in most neoplastic tissues.

Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P), IHC(F) and ICC.

Belongs to the MAGUK family.

TJP2 (Tight Junction Protein 2), also known as Zona Occludens 2 or ZO2 is a protein that in humans is encoded by the TJP2 gene. Tight junction proteins (TJPs) belong to a family of membrane-associated guanylate kinase (MAGUK) homologs that are involved in the organization of epithelial and endothelial intercellular junctions. Duclos et al. (1994) mapped the TJP2 gene telomeric to the Friedreich ataxia critical region on chromosome 9q13-q21. TJP2 lies about 70 kb centromeric to the X123 gene and is transcribed in the centromere-to-telomere direction. Using in vitro assays and immunoprecipitation studies, Itoh et al. (1999) showed that the mouse Tjp1, Tjp2, and Tjp3 PDZ1 domains interacted with the C-terminal cytoplasmic domains of Cldn1 through Cldn8. In the mouse inner ear, Walsh et al. (2010) found that Tjp2 expression decreased rapidly between E16.5 and age 1 week to a level in adult mice that was approximately 50% of the level at birth (P0).

Cell Adhesion, Cytoskeleton / Ecm, Signal Transduction, Tight Junctions