产品简要
公司名称 :
武汉博士德生物工程有限公司
产品类型 :
抗体
产品名称 :
Anti-Smad Interacting Protein 1/ZEB2 Antibody
目录 :
PA1959
规格 :
100μg/vial
价格 :
315美元
克隆性 :
多克隆
宿主 :
domestic rabbit
共轭标签 :
未共轭
反应物种 :
人类, 小鼠, 大鼠
应用 :
免疫印迹, 免疫组化-石蜡切片
更多信息或购买 :
武汉博士德生物工程有限公司 产品网页
图像
图像 1 :
武汉博士德生物工程有限公司 PA1959 图像 1
Anti-ZEB2 antibody, PA1959, Western blotting. Lane 1: 293T Cell Lysate. Lane 2: HELA Cell Lysate.
图像 2 :
武汉博士德生物工程有限公司 PA1959 图像 2
Figure 2. IHC analysis of ZEB2 using anti-ZEB2 antibody (PA1959). ZEB2 was detected in paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-ZEB2 Antibody (PA1959) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
图像 3 :
武汉博士德生物工程有限公司 PA1959 图像 3
Figure 3. IHC analysis of ZEB2 using anti-ZEB2 antibody (PA1959). ZEB2 was detected in paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-ZEB2 Antibody (PA1959) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
产品信息
SKU号 :
PA1959
产品名称 :
Anti-Smad Interacting Protein 1/ZEB2 Antibody
价格 :
315美元
规格 :
100μg/vial
克隆性 :
多克隆
宿主 :
兔
反应物种 :
人类, 小鼠, 大鼠
前体活性 :
仓鼠
应用(s) :
免疫组化-P, 免疫印迹
应用细节 :
Western blot, 0.1-0.5µg/ml, Human, Mouse, Rat. Immunohistochemistry (Paraffin-embedded Section), 0.5-1µg/ml, Human, Mouse, Rat, By Heat.
应用注释 :
WB: The detection limit for ZEB2 is approximately 0.5ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.
描述 :
Boster Bio Anti-Smad Interacting Protein 1/ZEB2 Antibody catalog # PA1959. Tested in IHC-P, WB applications. This antibody reacts with Human, Mouse, Rat.
浓度 :
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
基因名称 :
ZEB2
UniProt数据库编号 :
O60315
免疫原 :
A synthetic peptide corresponding to a sequence at the C-terminus of human ZEB2 (948-962aa DMQQRRKYQRKQGFQ), identical to the related rat and mouse sequences.
形式 :
冻干
Contents :
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
纯化 :
免疫原亲和纯化
交叉活性 :
No cross reactivity with other proteins
储存 :
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
重组 :
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
基因全称 :
锌指E拳击同源异形盒2
同义词 :
Zinc finger E-box-binding homeobox 2; Smad-interacting protein 1; SMADIP1; Zinc finger homeobox protein 1b; ZEB2; KIAA0569, SIP1, ZFHX1B, ZFX1B; HRIHFB2411;
蛋白名 :
锌指E拳击同源异形盒2
分子量 :
136447分子量
Protein Function :
Transcriptional inhibitor that binds to DNA sequence 5'- CACCT-3' in different promoters. Represses transcription of E- cadherin.
Subcellular Localization :
Nucleus.
Recommended Detection Systems :
Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB, supported by SA1022 in IHC(P).
Sequence Similarities :
Belongs to the delta-EF1/ZFH-1 C2H2-type zinc-finger family.
背景 :
ZEB2 (Zinc finger E-box-binding homeobox2), also known as SIP1or ZINC FINGER HOMEOBOX 1B (ZFHX1B), is a protein that in humans is encoded by the ZEB2 gene. The ZEB2 gene is a member of the ZEB1/Drosophila Zfh1 family of 2-handed zinc finger/homeodomain proteins and functions as a DNA-binding transcriptional repressor that interacts with activated SMADs, the transducers of TGF-beta signaling, and interacts with the nucleosome remodeling and histone deacetylation (NURD) complex. By radiation hybrid analysis, Nagase et al. (1998) mapped the ZEB2 gene to chromosome 2. Wakamatsu et al. (2001) mapped the ZEB2 gene to chromosome 2q22. Vandewalle et al. (2005) showed that expression of mouse Sip1 in human epithelial cells caused a morphologic change from an epithelial to a mesenchymal phenotype. Expression of SNAI1 in epithelial cells triggers an epithelial-mesenchyme transition. Beltran et al. (2008) showed that synthesis of ZEB2 was upregulated following SNAI1 expression in human cell lines.
研究种类 :
Developmental Families, Domain Families, Epigenetics and Nuclear Signaling, Lineage Markers, Lineage Specification, Neurogenesis, Neurology Process, Neuroscience, Oncoproteins, Oncoproteins/Suppressors, Stem Cells, Transcription, Transcription Factors
更多信息或购买 :
武汉博士德生物工程有限公司 产品网页
公司信息
武汉博士德生物工程有限公司
3942 B Valley Ave
Pleasanton, CA 94566
boster@bosterbio.com
http://www.boster.com.cn
925.485.4527
公司总部: 美国
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