产品简要
公司名称 :
武汉博士德生物工程有限公司
产品类型 :
抗体
产品名称 :
抗MBD4抗体
目录 :
PA2042
规格 :
100μg/vial
价格 :
315美元
克隆性 :
多克隆
宿主 :
domestic rabbit
共轭标签 :
未共轭
反应物种 :
人类, 小鼠, 大鼠
应用 :
免疫印迹
图像
图像 1 :
武汉博士德生物工程有限公司 PA2042 图像 1
Anti-MBD4 antibody, PA2042, Western blotting. Recombinant Protein Detection Source: E.coli derived -recombinant Human MBD4, 39.7KD (162aa tag+ Q400-S580). Lane 1: Recombinant Human MBD4 Protein 10ng . Lane 2: Recombinant Human MBD4 Protein 5ng . Lane 3: Recombinant Human MBD4 Protein 2.5ng
图像 2 :
武汉博士德生物工程有限公司 PA2042 图像 2
Anti-MBD4 antibody, PA2042, Western blotting. Lane 1: Rat Brain Tissue Lysate. Lane 2: Rat Kidney Tissue Lysate. Lane 3: A549 Cell Lysate. Lane 4: HELA Cell Lysate. Lane 5: MCF-7 Cell Lysate.
产品信息
SKU号 :
PA2042
产品名称 :
抗MBD4抗体
价格 :
315美元
规格 :
100μg/vial
克隆性 :
多克隆
宿主 :
反应物种 :
人类, 小鼠, 大鼠
前体活性 :
仓鼠
应用(s) :
免疫印迹
应用细节 :
免疫印迹, 0.1 0.5µg/毫升, 人类, 大鼠, 小鼠
应用注释 :
WB: The detection limit for MBD4 is approximately 1ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.
描述 :
Boster Bio Anti-MBD4 Antibody catalog # PA2042. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.
浓度 :
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
基因名称 :
MBD4
UniProt数据库编号 :
O95243
免疫原 :
A synthetic peptide corresponding to a sequence at the C-terminus of human MBD4 (566-580aa YHDWLWENHEKLSLS), identical to the related rat and mouse sequences.
形式 :
冻干
Contents :
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
纯化 :
免疫原亲和纯化
交叉活性 :
No cross reactivity with other proteins
储存 :
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
重组 :
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
基因全称 :
Methyl-CpG-binding domain protein 4
同义词 :
Methyl-CpG-binding domain protein 4; 3.2.2.-; Methyl-CpG-binding endonuclease 1; Methyl-CpG-binding protein MBD4; Mismatch-specific DNA N-glycosylase; MBD4; MED1;
蛋白名 :
Methyl-CpG-binding domain protein 4
分子量 :
66051分子量
Protein Function :
Mismatch-specific DNA N-glycosylase involved in DNA repair. Has thymine glycosylase activity and is specific for G:T mismatches within methylated and unmethylated CpG sites. Can also remove uracil or 5-fluorouracil in G:U mismatches. Has no lyase activity. Was first identified as methyl-CpG-binding protein.
Subcellular Localization :
Nucleus.
Recommended Detection Systems :
Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
Sequence Similarities :
Contains 1 MBD (methyl-CpG-binding) domain.
背景 :
MBD4 (Methyl-CpG-Binding Domain Protein 4), also known as MED1, is a protein that in humans is encoded by the MBD4 gene. MBD4 specifically binds methylated DNA, colocalizes with methylated sequences, and is likely to mediate the effects of DNA methylation in mammalian cells (Hendrich and Bird, 1998). Riccio et al. (1999) mapped the MBD4 gene to chromosome 3q21-q22 by FISH. Hendrich and Bird (1998) found that both MBD2 and MBD4 specifically bound methylated DNA in vitro and colocalized with methylated sequences in vivo. They concluded that MBD2 and MBD4 are likely to be mediators of the effects of DNA methylation in mammalian cells. Hendrich et al. (1999) showed that MBD4 contains a methyl-CpG-binding domain that can efficiently remove thymine or uracil from mismatched CpG sites in vitro. Furthermore, the methyl-CpG-binding domain of MBD4 binds preferentially to 5-methylcytosine CpG-TpG mismatches--the primary product of deamination at methyl-CpG.
研究种类 :
Epigenetics and Nuclear Signaling
公司信息
武汉博士德生物工程有限公司
3942 B Valley Ave
Pleasanton, CA 94566
boster@bosterbio.com
http://www.boster.com.cn
925.485.4527
公司总部: 美国
Premium Provider of Antibodies and ELISA Kits