产品简要
公司名称 :
武汉博士德生物工程有限公司
产品类型 :
抗体
产品名称 :
抗胶原I/COL1A1抗体
目录 :
PA2140-2
规格 :
100μg/vial
价格 :
315美元
克隆性 :
多克隆
宿主 :
domestic rabbit
共轭标签 :
未共轭
反应物种 :
人类, 小鼠, 大鼠
应用 :
免疫印迹, 免疫细胞化学, 免疫组化-石蜡切片, 免疫组化-冰冻切片
更多信息或购买 :
武汉博士德生物工程有限公司 产品网页
图像
图像 1 :
武汉博士德生物工程有限公司 PA2140-2 图像 1
Figure 1. IHC analysis of Collagen I/COL1A1 using anti- Collagen I/COL1A1 antibody (PA2140-2). Collagen I/COL1A1 was detected in paraffin-embedded section of Rat Lung tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti- Collagen I/COL1A1 Antibody (PA2140-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
图像 2 :
武汉博士德生物工程有限公司 PA2140-2 图像 2
Figure 2. IHC analysis of Collagen I/COL1A1 using anti- Collagen I/COL1A1 antibody (PA2140-2). Collagen I/COL1A1 was detected in paraffin-embedded section of Human Intestinal Cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti- Collagen I/COL1A1 Antibody (PA2140-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
图像 3 :
武汉博士德生物工程有限公司 PA2140-2 图像 3
Figure 3. IHC analysis of Collagen I/COL1A1 using anti- Collagen I/COL1A1 antibody (PA2140-2). Collagen I/COL1A1 was detected in immunocytochemical section of NIH3T3 Cell. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti- Collagen I/COL1A1 Antibody (PA2140-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
产品信息
SKU号 :
PA2140-2
产品名称 :
抗胶原I/COL1A1抗体
价格 :
315美元
规格 :
100μg/vial
克隆性 :
多克隆
宿主 :
兔
反应物种 :
人类, 小鼠, 大鼠
前体活性 :
仓鼠
应用(s) :
IF, 免疫组化-P, 免疫组化-F, 免疫细胞化学, 免疫印迹
应用细节 :
Immunofluorescence, 2μg/ml, HumanImmunohistochemistry (Paraffin-embedded Section), 0.5-1µg/ml, Human, Mouse, Rat, By Heat. Immunocytochemistry, 0.5-1µg/ml, Mouse, Human, RatImmunohistochemistry (Frozen Section), 0.5-1µg/ml, Human, Rat, Mouse Western blot, 0.1-0.5µg/ml, Human, Rat, Mouse.
应用注释 :
WB: The detection limit for COL1A1 is approximately 0.5ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.
描述 :
Boster Bio Anti-Collagen I/COL1A1 Antibody catalog # PA2140-2. Tested in IF, IHC-P, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.
浓度 :
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
基因名称 :
COL1A1
UniProt数据库编号 :
P11087
免疫原 :
A synthetic peptide corresponding to a sequence at the C-terminus of mouse Collagen I (1192-1207aa QPPQEKSQDGGRYYRA), identical to the related rat sequence, and different from the related human sequence by two amino acids.
形式 :
冻干
Contents :
Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.
纯化 :
免疫原亲和纯化
交叉活性 :
No cross reactivity with other proteins
储存 :
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
重组 :
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
基因全称 :
胶原甲1(I)链
同义词 :
胶原甲1(I)链;甲1Ⅰ型胶原;Col1a1;Cola1;
蛋白名 :
胶原甲1(I)链
分子量 :
138032分子量
Protein Function :
Type I collagen is a member of group I collagen (fibrillar forming collagen).
Subcellular Localization :
Secreted, extracellular space, extracellular matrix.
组织特异性 :
Forms the fibrils of tendon, ligaments and bones. In bones the fibrils are mineralized with calcium hydroxyapatite.
Recommended Detection Systems :
Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P), IHC(F) and ICC.
Sequence Similarities :
Belongs to the fibrillar collagen family.
背景 :
Collagen, type I, alpha 1, also known as COL1A1, is a human gene that encodes the major component of type I collagen, the fibrillar collagen found in most connective tissues, including cartilage. This gene is mapped to 17q21.33. This gene encodes the pro-alpha1 chains of type I collagen whose triple helix comprises two alpha1 chains and one alpha2 chain. Type I is a fibril-forming collagen found in most connective tissues and is abundant in bone, cornea, dermis and tendon. Mutations in this gene are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIA, Ehlers-Danlos syndrome Classical type, Caffey Disease and idiopathic osteoporosis.
研究种类 :
Collagen, Cytoskeleton / Ecm, Ecm Proteins, Extracellular Matrix, Mesenchymal Stem Cells, Signal Transduction, Stem Cells
更多信息或购买 :
武汉博士德生物工程有限公司 产品网页
公司信息
武汉博士德生物工程有限公司
3942 B Valley Ave
Pleasanton, CA 94566
boster@bosterbio.com
http://www.boster.com.cn
925.485.4527
公司总部: 美国
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