抗体

Anti-EpCAM Antibody Picoband™

PB10059

100μg/vial

315美元

多克隆

domestic rabbit

未共轭

免疫印迹, 酶联免疫吸附测定, 免疫细胞化学, 流式细胞仪, 免疫组化-石蜡切片

Anti-EpCAM Antibody Picoband™

315美元

100μg/vial

多克隆

兔

人类

酶联免疫吸附测定, 流式细胞仪, IF, 免疫组化-P, 免疫印迹

Western blot, 0.1-0.5µg/ml. Immunohistochemistry (Paraffin-embedded Section), 0.5-1µg/ml, By Heat. Immunofluorescence, 2.5µg/ml. Flow Cytometry, 1-3μg/1x10^6cells. ELISA, 0.1-0.5µg/ml.

Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Boster Bio Anti-EpCAM Antibody Picoband™ catalog # PB10059. Tested in ELISA, Flow Cytometry, IF, IHC-P, WB applications. This antibody reacts with Human.

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

EPCAM

P16422

different from the related mouse sequence by fifteen amino acids, and from the related rat sequence by sixteen ami
ELKHKAREKPYDSKSLRTALQKEITTRYQLDPKFITSIL
YENN),
A synthetic peptide corresponding to a sequence in the middle region of human EPCAM (147-189aa

冻干

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

免疫原亲和纯化

No cross reactivity with other proteins

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

上皮细胞 粘连分子

Epithelial cell adhesion molecule; Ep-CAM; Adenocarcinoma-associated antigen; Cell surface glycoprotein Trop-1; Epithelial cell surface antigen; Epithelial glycoprotein; EGP; Epithelial glycoprotein 314; EGP314; hEGP314; KS 1/4 antigen; KSA; Major gastrointestinal tumor-associated protein GA733-2; Tumor-associated calcium signal transducer 1; CD326; EPCAM; GA733-2, M1S2, M4S1, MIC18, TACSTD1, TROP1;

上皮细胞 粘连分子

34932分子量

May act as a physical homophilic interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium for providing immunological barrier as a first line of defense against mucosal infection. Plays a role in embryonic stem cells proliferation and differentiation. Up-regulates the expression of FABP5, MYC and cyclins A and E.

Lateral cell membrane ; Single-pass type I membrane protein. Cell junction, tight junction. Colocalizes with CLDN7 at the lateral cell membrane and tight junction.

Highly and selectively expressed by undifferentiated rather than differentiated embryonic stem cells (ESC). Levels rapidly diminish as soon as ESC's differentiate (at protein levels). Expressed in almost all epithelial cell membranes but not on mesodermal or neural cell membranes. Found on the surface of adenocarcinoma.

Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P), IHC(F) and ICC.

Epithelial cell adhesion molecule (EpCAM) is a transmembrane glycoprotein mediating Ca2+-independent homotypic cell–cell adhesion in epithelia. This gene encodes a carcinoma-associated antigen and is a member of a family that includes at least two type I membrane proteins. This antigen is expressed on most normal epithelial cells and gastrointestinal carcinomas and functions as a homotypic calcium-independent cell adhesion molecule. The antigen is being used as a target for immunotherapy treatment of human carcinomas. Mutations in this gene result in congenital tufting enteropathy.

Cell Type Markers, Tags & Cell Markers, Tumor Associated