产品简要
公司名称 :
武汉博士德生物工程有限公司
产品类型 :
抗体
产品名称 :
Anti-GNB1 Antibody Picoband™
目录 :
PB10067
规格 :
100μg/vial
价格 :
315美元
克隆性 :
多克隆
宿主 :
domestic rabbit
共轭标签 :
未共轭
反应物种 :
人类, 小鼠, 大鼠
应用 :
免疫印迹, 免疫细胞化学, 流式细胞仪, 免疫组化-石蜡切片, 免疫组化-冰冻切片
图像
图像 1 :
武汉博士德生物工程有限公司 PB10067 图像 1
Figure 1. Western blot analysis of GNB1 using anti-GNB1 antibody (PB10067). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, . Lane 2: mouse cardiac muscle tissue lysates, . Lane 3: HELA whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GNB1 antigen affinity purified polyclonal antibody (Catalog # PB10067) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GNB1 at approximately 37KD. The expected band size for GNB1 is at 37KD.
图像 2 :
武汉博士德生物工程有限公司 PB10067 图像 2
Figure 2. IHC analysis of GNB1 using anti-GNB1 antibody (PB10067). GNB1 was detected in paraffin-embedded section of mouse kidney tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-GNB1 Antibody (PB10067) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
图像 3 :
武汉博士德生物工程有限公司 PB10067 图像 3
Figure 3. IHC analysis of GNB1 using anti-GNB1 antibody (PB10067). GNB1 was detected in paraffin-embedded section of rat kidney tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-GNB1 Antibody (PB10067) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
产品信息
SKU号 :
PB10067
产品名称 :
Anti-GNB1 Antibody Picoband™
价格 :
315美元
规格 :
100μg/vial
克隆性 :
多克隆
宿主 :
反应物种 :
人类, 小鼠, 大鼠
前体活性 :
Bovine, Canine, Hamster, Horse, Monkey, Rabbit, Zebrafish
应用(s) :
流式细胞仪, IF, 免疫组化-P, 免疫组化-F, 免疫细胞化学, 免疫印迹
应用细节 :
Flow Cytometry, 1-3μg/1x10^6 cells, Human. Immunocytochemistry/Immunofluorescence, 2µg/ml, Human. Immunohistochemistry (Frozen Section), 0.5-1µg/ml, Human. Immunohistochemistry (Paraffin-embedded Section), 0.5-1µg/ml, Human, Mouse, Rat, By Heat. Western blot, 0.1-0.5µg/ml, Human, Mouse, Rat.
应用注释 :
Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.
描述 :
Boster Bio Anti-GNB1 Antibody Picoband™ catalog # PB10067. Tested in Flow Cytometry, IF, IHC-P, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.
浓度 :
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
基因名称 :
GNB1
UniProt数据库编号 :
P62873
免疫原 :
identical to the related mouse and rat sequences.
SELDQLRQEAEQLKNQIRDARKACADATLSQITNNIDPV
GR),
A synthetic peptide corresponding to a sequence at the N-terminus of human GNB1 (2-42aa
形式 :
冻干
Contents :
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
纯化 :
免疫原亲和纯化
交叉活性 :
No cross reactivity with other proteins
储存 :
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
重组 :
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
基因全称 :
鸟嘌呤核苷酸结合蛋白G(I)/G(S)/G(T)亚单位乙1
同义词 :
鸟嘌呤核苷酸结合蛋白G(I)/G(S)/G(T)亚单位乙1;转导乙链1;GNB1;
蛋白名 :
鸟嘌呤核苷酸结合蛋白G(I)/G(S)/G(T)亚单位乙1
分子量 :
37377分子量
Protein Function :
Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein- effector interaction.
Recommended Detection Systems :
Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P), IHC(F) and ICC.
背景 :
Guanine nucleotide-binding protein G (I)/G (S)/G (T) subunit beta-1 is a protein that in humans is encoded by the GNB1 gene. Heterotrimeric guanine nucleotide-binding proteins (G proteins), which integrate signals between receptors and effector proteins, are composed of an alpha, a beta, and a gamma subunit. These subunits are encoded by families of related genes. This gene encodes a beta subunit. Beta subunits are important regulators of alpha subunits, as well as of certain signal transduction receptors and effectors. This gene uses alternative polyadenylation signals.
研究种类 :
Cancer, Cancer Metabolism, Energy Transfer Pathways, G Protein Signaling, Heterotrimeric G Proteins, Integration Of Energy, Integration Of Energy Metabolism, Metabolic Signaling Pathway, Metabolic Signaling Pathways, Metabolism, Pathways And Processes, Signal Transduction, Signaling Pathway
公司信息
武汉博士德生物工程有限公司
3942 B Valley Ave
Pleasanton, CA 94566
boster@bosterbio.com
http://www.boster.com.cn
925.485.4527
公司总部: 美国
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