产品简要
公司名称 :
武汉博士德生物工程有限公司
产品类型 :
抗体
产品名称 :
Anti-Progesterone Receptor/PGR Picoband Antibody
目录 :
PB9009
规格 :
100 ug/vial
价格 :
280美元
克隆性 :
多克隆
宿主 :
共轭标签 :
nonconjugated
反应物种 :
人类
应用 :
免疫印迹, 免疫组化
图像
图像 1 :
武汉博士德生物工程有限公司 PB9009 图像 1
Figure 1. IHC analysis of Progesterone Receptor using anti-Progesterone Receptor antibody (PB9009). Progesterone Receptor was detected in paraffin-embedded section of human mammary cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-Progesterone Receptor Antibody (PB9009) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
图像 2 :
武汉博士德生物工程有限公司 PB9009 图像 2
Figure 2. Western blot analysis of Progesterone Receptor using anti-Progesterone Receptor antibody (PB9009). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human T-47D whole cell lysates, . After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Progesterone Receptor antigen affinity purified polyclonal antibody (Catalog # PB9009) at 0.1 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Progesterone Receptor at approximately 90 KD (PR-A), 118KD (PR-B). The expected band size for Progesterone Receptor is at 99KD.
产品信息
SKU号 :
PB9009
状态 :
Enabled
名称 :
Anti-Progesterone Receptor/PGR Picoband Antibody
目录名称 :
一抗, 多克隆抗体, 免疫组化免疫细胞化学IF抗体
基因名称 :
PGR
价格 :
280美元
price various sizes :
Unconjugated / $280 APC / $330 APC-Cy7 / $330 FITC / $330 PE / $330 PE-Cy5 / $330 PE-Cy7 / $330 30ug sample size / $99 100ug / $280 100ug+Free HRP Secondary BA1054 / $280 100ug+Free Biotin Secondary BA1003 / $280
克隆性 :
多克隆
浓度 :
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
共轭标签 :
No
contents :
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
描述 :
Polyclonal antibody for NR3C3/PGR detection. Host: Rabbit.Size: 100ug/vial. Tested applications: WB, IHC-P. Reactive species: Human. NR3C3/PGR information: Molecular Weight: 98981 MW; Subcellular Localization: Nucleus. Cytoplasm. Nucleoplasmic shuttling is both homone- and cell cycle-dependent. On hormone stimulation, retained in the cytoplasm in the G(1) and G(2)/M phases.
short description :
Rabbit IgG polyclonal antibody for Progesterone receptor(PGR) detection. Tested with WB, IHC-P in Human.
规格 :
100 ug/vial
sample size available :
30ug for $99, contact us for details
UniProt数据库编号 :
P06401
宿主 :
免疫原 :
E.coli-derived human Progesterone Receptor recombinant protein (Position: M595-K933). Human Progesterone Receptor shares 95% and 94% amino acid (aa) sequences identity with mouse and rat Progesterone Receptor, respectively.
形式 :
冻干
纯化 :
免疫原亲和纯化
储存 :
At -20 C for one year. After reconstitution, at 4 C for one month. It can also be aliquotted and stored frozen at -20 C for a longer time.Avoid repeated freezing and thawing.
交叉活性 :
No cross reactivity with other proteins
重组 :
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
应用细节 :
Western blot, 0.1-0.5ug/ml, Human. Immunohistochemistry(Paraffin-embedded Section), 0.5-1ug/ml, Human, By Heat.
应用 :
免疫组化, 免疫印迹
反应物种 :
人类
图像标记 :
Figure 1. IHC analysis of Progesterone Receptor using anti-Progesterone Receptor antibody (PB9009). Progesterone Receptor was detected in paraffin-embedded section of human mammary cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-Progesterone Receptor Antibody (PB9009) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. Figure 2. Western blot analysis of Progesterone Receptor using anti-Progesterone Receptor antibody (PB9009). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human T-47D whole cell lysates, . After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Progesterone Receptor antigen affinity purified polyclonal antibody (Catalog # PB9009) at 0.1 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Progesterone Receptor at approximately 90 KD (PR-A), 118KD (PR-B). The expected band size for Progesterone Receptor is at 99KD.
背景 :
The progesterone receptor (PR) is an intracellular steroid receptor that specially binds progesterone in humans. PR has been a member of the steroid receptor superfamily. It is encoded by a single PGR gene residing on chromosome 11q22. The PGR gene uses separate promoters and translational start sites to produce 2 isoforms, PRA and PRB, which are identical except for an additional 165 amino acids present only in the N terminus of PRB. It can be observed in human breast tissues. The proteins function as dimeric molecules in nuclei to regulate the transcription of target genes in a ligand-responsive manner.
competitor equivalent skus :
sc 101786 sc 130071 sc 166169 sc 166169 X sc 166170 sc 166170 X sc 398898 sc 398898 X sc 538 sc 538 X sc 539 sc 539 X sc 53943 sc 56862 sc 57553 sc 7208 sc 7208 X sc 810 sc 810 X sc 811 sc 811 X
研究种类 :
Cancer, Domain Families, Endocrine System, Epigenetics And Nuclear Signaling, Neuroscience, Nuclear Hormone Receptors, Nuclear Receptors, Nuclear Signaling Pathways, Signal Transduction, Signaling Pathway, Transcription, Tumor Biomarkers, Zinc Finger
同义词 :
Progesterone receptor;PR;Nuclear receptor subfamily 3 group C member 3;PGR;NR3C3;
基因全称 :
孕激素受体
分子量 :
98981分子量
protein function :
The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Progesterone receptor isoform B (PRB) is involved activation of c-SRC/MAPK signaling on hormone stimulation.
subcellular localization :
Nucleus. Cytoplasm. Nucleoplasmic shuttling is both homone- and cell cycle-dependent. On hormone stimulation, retained in the cytoplasm in the G(1) and G(2)/M phases.
蛋白名 :
孕激素受体
recommended detection systems :
Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
sequence similarities :
Belongs to the nuclear hormone receptor family. NR3 subfamily.
更新 :
3/18/19 7:09
公司信息
武汉博士德生物工程有限公司
3942 B Valley Ave
Pleasanton, CA 94566
boster@bosterbio.com
http://www.boster.com.cn
925.485.4527
公司总部: 美国
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