产品简要
公司名称 :
武汉博士德生物工程有限公司
产品类型 :
抗体
产品名称 :
Anti-Adiponectin/ADIPOQ Antibody Picoband‚Ñ¢
目录 :
PB9011
规格 :
10微克样本
价格 :
99美元
克隆性 :
多克隆
宿主 :
domestic rabbit
共轭标签 :
未共轭
反应物种 :
小鼠, 大鼠
应用 :
免疫印迹, 免疫组化
图像
图像 1 :
武汉博士德生物工程有限公司 PB9011 图像 1
Figure 1. IHC analysis of Adiponectin using anti-Adiponectin antibody (PB9011). Adiponectin was detected in paraffin-embedded section of mouse lung tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-Adiponectin Antibody (PB9011) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
图像 2 :
武汉博士德生物工程有限公司 PB9011 图像 2
Figure 2. IHC analysis of Adiponectin using anti-Adiponectin antibody (PB9011). Adiponectin was detected in paraffin-embedded section of mouse liver tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-Adiponectin Antibody (PB9011) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
图像 3 :
武汉博士德生物工程有限公司 PB9011 图像 3
Figure 3. IHC analysis of Adiponectin using anti-Adiponectin antibody (PB9011). Adiponectin was detected in paraffin-embedded section of rat intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-Adiponectin Antibody (PB9011) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
产品信息
SKU号 :
PB9011
状态 :
1
名称 :
Anti-Adiponectin/ADIPOQ Antibody Picoband‚Ñ¢
url key :
anti-adiponectin-picoband-trade-antibody-pb9011-boster
产品种类 :
一抗
基因名称 :
ADIPOQ
价格 :
315
规格1 :
10微克样本
price for size 1 :
99美元
规格2 :
Full size
price for size 2 :
315
规格3 :
Full size carrier free
price for size 3 :
365
克隆性 :
多克隆
浓度 :
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
contents :
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
描述 :
Boster Bio Anti-Adiponectin/ADIPOQ Antibody Picoband catalog # PB9011. Tested in IHC, WB applications. This antibody reacts with Mouse, Rat.
short description :
Boster Bio Anti-Adiponectin/ADIPOQ Antibody Picoband catalog # PB9011. Tested in IHC, WB applications. This antibody reacts with Mouse, Rat.
规格 :
100μg/vial
UniProt数据库编号 :
Q8K3R4
宿主 :
免疫原 :
E.coli-derived rat Adiponectin recombinant protein (Position: I19-N244). Rat Adiponectin shares 90% amino acid (aa) sequence identity with mouse Adiponectin.
形式 :
冻干
纯化 :
免疫原亲和纯化
储存 :
Store at -20ÀöC for one year from date of receipt. After reconstitution, at 4ÀöC for one month. It can also be aliquotted and stored frozen at -20ÀöC for six months. Avoid repeated freeze-thaw cycles.
交叉活性 :
No cross reactivity with other proteins
重组 :
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
应用细节 :
Immunohistochemistry (Paraffin-embedded Section), 0.5-1ug/ml, Mouse, Rat, By Heat . Western blot, 0.1-0.5ug/ml, Rat.
应用 :
免疫组化, 免疫印迹
反应物种 :
小鼠, 大鼠
images :
/antibody/pb9011-1-IHC-anti-adiponectin-picoband-antibody.jpg /antibody/pb9011-2-IHC-anti-adiponectin-picoband-antibody.jpg /antibody/pb9011-3-IHC-anti-adiponectin-picoband-antibody.jpg /antibody/pb9011-4-IHC-anti-adiponectin-picoband-antibody.jpg /antibody/pb9011-5-IHC-anti-adiponectin-picoband-antibody.jpg /antibody/pb9011-6-WB-anti-adiponectin-picoband-antibody.jpg
图像标记 :
Figure 1. IHC analysis of Adiponectin using anti-Adiponectin antibody (PB9011). Adiponectin was detected in paraffin-embedded section of mouse lung tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6 epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Adiponectin Antibody (PB9011) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. Figure 2. IHC analysis of Adiponectin using anti-Adiponectin antibody (PB9011). Adiponectin was detected in paraffin-embedded section of mouse liver tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6 epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Adiponectin Antibody (PB9011) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. Figure 3. IHC analysis of Adiponectin using anti-Adiponectin antibody (PB9011). Adiponectin was detected in paraffin-embedded section of rat intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6 epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Adiponectin Antibody (PB9011) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. Figure 4. IHC analysis of Adiponectin using anti-Adiponectin antibody (PB9011). Adiponectin was detected in paraffin-embedded section of rat lung tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6 epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Adiponectin Antibody (PB9011) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. Figure 5. IHC analysis of Adiponectin using anti-Adiponectin antibody (PB9011). Adiponectin was detected in paraffin-embedded section of rat liver tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6 epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Adiponectin Antibody (PB9011) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. Figure 6. Western blot analysis of Adiponectin using anti-Adiponectin antibody (PB9011). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: Rat Testis Tissue Lysate . Lane 2: Rat Spleen Tissue Lysate . Lane 3: Rat Skeletal Muscle Tissue Lysate . Lane 4: Rat Cardiac Muscle Tissue Lysate . Lane 5: Rat Liver Tissue Lysate . After Electrophoresis proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Adiponectin antigen affinity purified polyclonal antibody (Catalog # PB9011) at 0.5 μg/mL overnight at 4°C then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Adiponectin at approximately 26KD. The expected band size for Adiponectin is at 26KD.
custom options :
name=Conjugation,required=0,price=0.0000,type=drop_down,sku=,max_characters=0,file_extension=,image_size_x=0,image_size_y=0,price_type=fixed,option_title=Unconjugated name=Conjugation,required=0,price=100.0000,type=drop_down,sku=,max_characters=0,file_extension=,image_size_x=0,image_size_y=0,price_type=fixed,option_title=Alexa Fluor 488 name=Conjugation,required=0,price=50.0000,type=drop_down,sku=,max_characters=0,file_extension=,image_size_x=0,image_size_y=0,price_type=fixed,option_title=Biotin name=Conjugation,required=0,price=50.0000,type=drop_down,sku=,max_characters=0,file_extension=,image_size_x=0,image_size_y=0,price_type=fixed,option_title=HRP name=Conjugation,required=0,price=50.0000,type=drop_down,sku=,max_characters=0,file_extension=,image_size_x=0,image_size_y=0,price_type=fixed,option_title=Cy3 name=Conjugation,required=0,price=50.0000,type=drop_down,sku=,max_characters=0,file_extension=,image_size_x=0,image_size_y=0,price_type=fixed,option_title=Dylight 350 name=Conjugation,required=0,price=50.0000,type=drop_down,sku=,max_characters=0,file_extension=,image_size_x=0,image_size_y=0,price_type=fixed,option_title=Dylight 488 name=Conjugation,required=0,price=50.0000,type=drop_down,sku=,max_characters=0,file_extension=,image_size_x=0,image_size_y=0,price_type=fixed,option_title=Dylight 550 name=Conjugation,required=0,price=50.0000,type=drop_down,sku=,max_characters=0,file_extension=,image_size_x=0,image_size_y=0,price_type=fixed,option_title=Dylight 594 name=Conjugation,required=0,price=50.0000,type=drop_down,sku=,max_characters=0,file_extension=,image_size_x=0,image_size_y=0,price_type=fixed,option_title=FITC name=Conjugation,required=0,price=50.0000,type=drop_down,sku=,max_characters=0,file_extension=,image_size_x=0,image_size_y=0,price_type=fixed,option_title=PE name=Conjugation,required=0,price=50.0000,type=drop_down,sku=,max_characters=0,file_extension=,image_size_x=0,image_size_y=0,price_type=fixed,option_title=PE-Allo 594 name=Conjugation,required=0,price=50.0000,type=drop_down,sku=,max_characters=0,file_extension=,image_size_x=0,image_size_y=0,price_type=fixed,option_title=PE-Cy5 name=Size,required=1,price=-216.0000,type=radio,sku=,max_characters=0,file_extension=,image_size_x=0,image_size_y=0,price_type=fixed,option_title=10ug sample name=Size,required=1,price=0.0000,type=radio,sku=,max_characters=0,file_extension=,image_size_x=0,image_size_y=0,price_type=fixed,option_title=Full size name=Size,required=1,price=50.0000,type=radio,sku=,max_characters=0,file_extension=,image_size_x=0,image_size_y=0,price_type=fixed,option_title=Full size carrier free
created at :
4/9/14 15:19
updated at :
3/2/22 2:55
meta title :
脂联素/ADIPOQ抗体PB9011
meta keyword :
Anti-Adiponectin/ADIPOQ Antibody Picoband
meta description :
Boster Bio Anti-Adiponectin/ADIPOQ Antibody Picoband catalog # PB9011. Tested in IHC, WB applications. This antibody reacts with Mouse, Rat. Supplied as 100ug/vial in Lyophilized form antibody.
应用注释 :
Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.
rating value :
100
背景 :
Adiponectin, also known as APM1 or ACDC, is a protein which in humans is encoded by the ADIPOQ gene. It is mapped to chromosome 16 in a region showing homology of synteny with human 3q27. By RNase protection and Western blot analysis, it has been showed that Adiponectin is expressed by differentiated adipocytes as a 33-kD protein that is also detectable in serum. By sequence comparisons, it has been found that links between APM1 and TNF family ligands as well as to cytokines expressed by T cells. Adiponectin is a protein hormone that modulates a number of metabolic processes, including glucose regulation and fatty acid oxidation. This gene is exclusively secreted from adipose tissue (and also from the placenta in pregnancy) into the bloodstream and is very abundant in plasma relative to many hormones.
competitor equivalent skus :
sc 54687 sc 100610 sc 30838 sc 54479 sc 98953 sc 30840 sc 377072 sc 136115
研究种类 :
Atherosclerosis,Cancer,Cancer Metabolism,Cardiovascular,Diabetes,Diabetes Associated,Growth Factors/Hormones,Heart Disease,Lipids / Lipoproteins,Mesenchymal Stem Cells,Metabolism,Metabolism Processes,Neurology Process,Neuroscience,Obesity,Pathways And Processes,Response To Hypoxia,Signal Transduction,Stem Cells
同义词 :
30 kDa adipocyte complement-related protein ;Adiponectin, C1Q and collagen domain containing ;Protein Adipoq ;Adipoq ;Acdc ;rCG_36676 ;
基因全称 :
脂联素, C1Q和胶原区域含
分子量 :
26410分子量
蛋白名 :
30 kDa adipocyte complement-related protein
recommended detection systems :
Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
template :
抗体
公司信息
武汉博士德生物工程有限公司
3942 B Valley Ave
Pleasanton, CA 94566
boster@bosterbio.com
http://www.boster.com.cn
925.485.4527
公司总部: 美国
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