产品简要
公司名称 :
武汉博士德生物工程有限公司
产品类型 :
抗体
产品名称 :
Anti-CNTF Antibody Picoband‚Ñ¢
目录 :
PB9012
规格 :
10微克样本
价格 :
99美元
克隆性 :
多克隆
宿主 :
domestic rabbit
共轭标签 :
未共轭
反应物种 :
人类
应用 :
免疫印迹, 免疫组化
图像
图像 1 :
武汉博士德生物工程有限公司 PB9012 图像 1
Anti-CNTF Picoband antibody, PB9012-2.JPG. IHC(P): Human Intestinal Cancer Tissue
图像 2 :
武汉博士德生物工程有限公司 PB9012 图像 2
Anti-CNTF Picoband antibody, PB9012-3.JPG. IHC(P): Human Lung Cancer Tissue
图像 3 :
武汉博士德生物工程有限公司 PB9012 图像 3
Anti-CNTF Picoband antibody, PB9012-4.jpg. All lanes: Anti-CNTF(PB9012) at 0.5ug/ml. WB: Recombinant Human CNTF Protein 0.5ng. Predicted bind size: 28KD. Observed bind size: 28KD.
产品信息
SKU号 :
PB9012
状态 :
1
名称 :
Anti-CNTF Antibody Picoband‚Ñ¢
url key :
anti-cntf-picoband-trade-antibody-pb9012-boster
产品种类 :
一抗
基因名称 :
CNTF
价格 :
315
规格1 :
10微克样本
price for size 1 :
99美元
规格2 :
Full size
price for size 2 :
315
规格3 :
Full size carrier free
price for size 3 :
365
克隆性 :
多克隆
浓度 :
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
contents :
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
描述 :
Boster Bio Anti-CNTF Antibody Picoband catalog # PB9012. Tested in IHC, WB applications. This antibody reacts with Human.
short description :
Boster Bio Anti-CNTF Antibody Picoband catalog # PB9012. Tested in IHC, WB applications. This antibody reacts with Human.
规格 :
100μg/vial
UniProt数据库编号 :
P26441
宿主 :
免疫原 :
E.coli-derived human CNTF recombinant protein (Position: A2-M200). Human CNTF shares 83% and 84% amino acid (aa) sequences identity with mouse and rat CNTF, respectively.
形式 :
冻干
纯化 :
免疫原亲和纯化
储存 :
Store at -20ÀöC for one year from date of receipt. After reconstitution, at 4ÀöC for one month. It can also be aliquotted and stored frozen at -20ÀöC for six months. Avoid repeated freeze-thaw cycles.
交叉活性 :
No cross reactivity with other proteins
重组 :
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
应用细节 :
Immunohistochemistry (Paraffin-embedded Section), 0.5-1ug/ml, Human, By Heat. Western blot, 0.1-0.5ug/ml, Human.
应用 :
免疫组化, 免疫印迹
反应物种 :
人类
images :
/p/b/pb9012-1-ihc-anti-cntf-picoband-antibody_1_.jpg /p/b/pb9012-2-ihc-anti-cntf-picoband-antibody.jpg /p/b/pb9012-3-wb-anti-cntf-picoband-antibody.jpg
图像标记 :
Figure 1. IHC analysis of CNTF using anti-CNTF antibody (PB9012). CNTF was detected in paraffin-embedded section of human intestinal cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CNTF Antibody (PB9012) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. Figure 2. IHC analysis of CNTF using anti-CNTF antibody (PB9012). CNTF was detected in paraffin-embedded section of human lung cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CNTF Antibody (PB9012) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. Figure 3. Western blot analysis of CNTF using anti-CNTF antibody (PB9012). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Recombinant Human CNTF Protein 0.5ng. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CNTF antigen affinity purified polyclonal antibody (Catalog # PB9012) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CNTF at approximately 28KD. The expected band size for CNTF is at 28KD.
custom options :
name=Conjugation,required=0,price=0.0000,type=drop_down,sku=,max_characters=0,file_extension=,image_size_x=0,image_size_y=0,price_type=fixed,option_title=Unconjugated 100μg name=Conjugation,required=0,price=100.0000,type=drop_down,sku=,max_characters=0,file_extension=,image_size_x=0,image_size_y=0,price_type=fixed,option_title=Alexa Fluor 488 50μg name=Conjugation,required=0,price=50.0000,type=drop_down,sku=,max_characters=0,file_extension=,image_size_x=0,image_size_y=0,price_type=fixed,option_title=Biotin 50μg name=Conjugation,required=0,price=50.0000,type=drop_down,sku=,max_characters=0,file_extension=,image_size_x=0,image_size_y=0,price_type=fixed,option_title=HRP 50μg name=Conjugation,required=0,price=50.0000,type=drop_down,sku=,max_characters=0,file_extension=,image_size_x=0,image_size_y=0,price_type=fixed,option_title=Cy3 50μg name=Conjugation,required=0,price=50.0000,type=drop_down,sku=,max_characters=0,file_extension=,image_size_x=0,image_size_y=0,price_type=fixed,option_title=Dylight 350 50μg name=Conjugation,required=0,price=50.0000,type=drop_down,sku=,max_characters=0,file_extension=,image_size_x=0,image_size_y=0,price_type=fixed,option_title=Dylight 488 50μg name=Conjugation,required=0,price=50.0000,type=drop_down,sku=,max_characters=0,file_extension=,image_size_x=0,image_size_y=0,price_type=fixed,option_title=Dylight 550 50μg name=Conjugation,required=0,price=50.0000,type=drop_down,sku=,max_characters=0,file_extension=,image_size_x=0,image_size_y=0,price_type=fixed,option_title=Dylight 594 50μg name=Conjugation,required=0,price=50.0000,type=drop_down,sku=,max_characters=0,file_extension=,image_size_x=0,image_size_y=0,price_type=fixed,option_title=FITC 50μg name=Conjugation,required=0,price=50.0000,type=drop_down,sku=,max_characters=0,file_extension=,image_size_x=0,image_size_y=0,price_type=fixed,option_title=PE 50μg name=Conjugation,required=0,price=50.0000,type=drop_down,sku=,max_characters=0,file_extension=,image_size_x=0,image_size_y=0,price_type=fixed,option_title=PE-Allo 594 50μg name=Conjugation,required=0,price=50.0000,type=drop_down,sku=,max_characters=0,file_extension=,image_size_x=0,image_size_y=0,price_type=fixed,option_title=PE-Cy5 50μg name=Size,required=1,price=-216.0000,type=radio,sku=,max_characters=0,file_extension=,image_size_x=0,image_size_y=0,price_type=fixed,option_title=10ug sample name=Size,required=1,price=0.0000,type=radio,sku=,max_characters=0,file_extension=,image_size_x=0,image_size_y=0,price_type=fixed,option_title=Full size name=Size,required=1,price=50.0000,type=radio,sku=,max_characters=0,file_extension=,image_size_x=0,image_size_y=0,price_type=fixed,option_title=Full size carrier free
created at :
4/9/14 15:19
updated at :
3/2/22 2:55
meta title :
CNTF抗体PB9012
meta keyword :
Anti-CNTF Antibody Picoband
meta description :
Boster Bio Anti-CNTF Antibody Picoband catalog # PB9012. Tested in IHC, WB applications. This antibody reacts with Human. Supplied as 100ug/vial in Lyophilized form antibody.
应用注释 :
WB: The detection limit for CNTF is approximately 0.25ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.
rating value :
100
背景 :
Ciliary neurotrophic factor (CNTF) is a potent polypeptide hormone whose actions appear to be restricted to the nervous system where it promotes survival, neurotransmitter synthesis and neurite outgrowth in certain neuronal populations. The CNTF gene is located on chromosome 11, as determined using human-hamster somatic cell hybrids. The CNTF protein is highly conserved in evolution. The amino acid (aa) sequences of rat and rabbit CNTF translated from cDNAs display approx. 85% homology with the deduced aa sequence encoding human CNTF. CNTF induces weight loss and improves glucose tolerance in humans and rodents. CNTF is thought to act centrally by inducing hypothalamic neurogenesis to modulate food intake and peripherally by altering hepatic gene expression, in a manner similar to that of leptin.
competitor equivalent skus :
sc 13996 sc 1912 sc 365211 sc 166272 sc 365210 sc 1911 sc 166273 sc 25286 sc 1912 R
研究种类 :
Cell Type Marker,Growth And Development,Neural Stem Cells,Neurology Process,Neuroscience,Stem Cells
同义词 :
睫状神经营养因子;CNTF;CNTF;
基因全称 :
睫状神经营养因子
分子量 :
22931分子量
protein function :
CNTF is a survival factor for various neuronal cell types. Seems to prevent the degeneration of motor axons after axotomy.
subcellular localization :
细胞质
组织特异性 :
神经系统
蛋白名 :
睫状神经营养因子
recommended detection systems :
Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
sequence similarities :
Belongs to the CNTF family.
template :
抗体
公司信息
武汉博士德生物工程有限公司
3942 B Valley Ave
Pleasanton, CA 94566
boster@bosterbio.com
http://www.boster.com.cn
925.485.4527
公司总部: 美国
Premium Provider of Antibodies and ELISA Kits