产品简要
公司名称 :
武汉博士德生物工程有限公司
产品类型 :
抗体
产品名称 :
Anti-IL18 Antibody Picoband‚Ñ¢
目录 :
PB9014
规格 :
10微克样本
价格 :
99美元
克隆性 :
多克隆
宿主 :
domestic rabbit
共轭标签 :
未共轭
反应物种 :
大鼠
应用 :
免疫印迹, 酶联免疫吸附测定, 流式细胞仪, 免疫组化-石蜡切片
图像
图像 1 :
武汉博士德生物工程有限公司 PB9014 图像 1
Figure 1. Western blot analysis of IL18 using anti-IL18 antibody (PB9014). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, . Lane 2: rat lung tissue lysates, . Lane 3: rat spleen tissue lysates. Lane 4: rat liver tissue lysates. Lane 5: rat testis tissue lysates. Lane 6: rat RH35 lysates. Lane 7: rat PC-12 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL18 antigen affinity purified polyclonal antibody (Catalog # PB9014) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL18 at approximately 22KD. The expected band size for IL18 is at 22KD.
产品信息
SKU号 :
PB9014
状态 :
1
名称 :
Anti-IL18 Antibody Picoband‚Ñ¢
url key :
抗白细胞介素18
产品种类 :
一抗
基因名称 :
白细胞介素-18
价格 :
315
规格1 :
10微克样本
price for size 1 :
99美元
规格2 :
Full size
price for size 2 :
315
规格3 :
Full size carrier free
price for size 3 :
365
克隆性 :
多克隆
浓度 :
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
contents :
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2mg Na2HPO4, 0.05 mg NaN3.
描述 :
Boster Bio Anti-IL18 Antibody Picoband catalog # PB9014. Tested in ELISA, Flow Cytometry, IHC-P, WB applications. This antibody reacts with Mouse, Rat.
short description :
Boster Bio Anti-IL18 Antibody Picoband catalog # PB9014. Tested in ELISA, Flow Cytometry, IHC-P, WB applications. This antibody reacts with Mouse, Rat.
规格 :
100μg/vial
UniProt数据库编号 :
P97636
宿主 :
免疫原 :
E.coli-derived rat IL-18 recombinant protein (Position: H37-S194). Rat IL-18 shares 64% and 91% amino acid (aa) sequences identity with human and mouse IL-18, respectively.
形式 :
冻干
纯化 :
免疫原亲和纯化
储存 :
Store at -20ÀöC for one year from date of receipt. After reconstitution, at 4ÀöC for one month. It can also be aliquotted and stored frozen at -20ÀöC for six months. Avoid repeated freeze-thaw cycles.
交叉活性 :
No cross reactivity with other proteins
重组 :
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
应用细节 :
Western blot, 0.25-0.5ug/ml, Mouse, Rat. Immunohistochemistry (Paraffin-embedded Section), 2-5ug/ml, Rat, By Heat. Flow Cytometry, 1-3μg/1x10 6 cells, Rat. Direct ELISA, 0.1-0.5ug/ml, Rat
应用 :
酶联免疫吸附测定, 流式细胞仪, 免疫组化-P, 免疫印迹
反应物种 :
小鼠, 大鼠
images :
/p/b/pb9014-il18-primary-antibodies-wb-testing-1.jpg /p/b/pb9014-il18-primary-antibodies-fcm-testing-2.png /p/b/pb9014-il18-primary-antibodies-ihc-testing-3.jpg
图像标记 :
Figure 1. Western blot analysis of IL18 using anti-IL18 antibody (PB9014). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: mouse spleen tissue lysates, . Lane 2: mouse thymus tissue lysates, . Lane 3: mouse lung tissue lysates, . Lane 4: mouse ANA-1 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL18 antigen affinity purified polyclonal antibody (Catalog # PB9014) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL18 at approximately 18KD. The expected band size for IL18 is at 18KD. Figure 2. Flow Cytometry analysis of RH35 cells using anti-IL18 antibody (PB9014). Overlay histogram showing RH35 cells stained with PB9014 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-IL18 Antibody (PB9014, 1μg/1x10 6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10 6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10 6 ) used under the same conditions. Unlabelled sample (Red line) was also used as a control. Figure 3. IHC analysis of IL18 using anti-IL18 antibody (PB9014). IL18 was detected in paraffin-embedded section of rat spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-IL18 Antibody (PB9014) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
custom options :
name=Size,required=1,price=-216.0000,type=radio,sku=,max_characters=0,file_extension=,image_size_x=0,image_size_y=0,price_type=fixed,option_title=10ug sample name=Size,required=1,price=0.0000,type=radio,sku=,max_characters=0,file_extension=,image_size_x=0,image_size_y=0,price_type=fixed,option_title=Full size name=Size,required=1,price=50.0000,type=radio,sku=,max_characters=0,file_extension=,image_size_x=0,image_size_y=0,price_type=fixed,option_title=Full size carrier free name=Conjugation,required=0,price=0.0000,type=drop_down,sku=,max_characters=0,file_extension=,image_size_x=0,image_size_y=0,price_type=fixed,option_title=Unconjugated 100μg name=Conjugation,required=0,price=100.0000,type=drop_down,sku=,max_characters=0,file_extension=,image_size_x=0,image_size_y=0,price_type=fixed,option_title=Alexa Fluor 488 50μg name=Conjugation,required=0,price=50.0000,type=drop_down,sku=,max_characters=0,file_extension=,image_size_x=0,image_size_y=0,price_type=fixed,option_title=Biotin 50μg name=Conjugation,required=0,price=50.0000,type=drop_down,sku=,max_characters=0,file_extension=,image_size_x=0,image_size_y=0,price_type=fixed,option_title=HRP 50μg name=Conjugation,required=0,price=50.0000,type=drop_down,sku=,max_characters=0,file_extension=,image_size_x=0,image_size_y=0,price_type=fixed,option_title=Cy3 50μg name=Conjugation,required=0,price=50.0000,type=drop_down,sku=,max_characters=0,file_extension=,image_size_x=0,image_size_y=0,price_type=fixed,option_title=Dylight 350 50μg name=Conjugation,required=0,price=50.0000,type=drop_down,sku=,max_characters=0,file_extension=,image_size_x=0,image_size_y=0,price_type=fixed,option_title=Dylight 488 50μg name=Conjugation,required=0,price=50.0000,type=drop_down,sku=,max_characters=0,file_extension=,image_size_x=0,image_size_y=0,price_type=fixed,option_title=Dylight 550 50μg name=Conjugation,required=0,price=50.0000,type=drop_down,sku=,max_characters=0,file_extension=,image_size_x=0,image_size_y=0,price_type=fixed,option_title=Dylight 594 50μg name=Conjugation,required=0,price=50.0000,type=drop_down,sku=,max_characters=0,file_extension=,image_size_x=0,image_size_y=0,price_type=fixed,option_title=FITC 50μg name=Conjugation,required=0,price=50.0000,type=drop_down,sku=,max_characters=0,file_extension=,image_size_x=0,image_size_y=0,price_type=fixed,option_title=PE 50μg name=Conjugation,required=0,price=50.0000,type=drop_down,sku=,max_characters=0,file_extension=,image_size_x=0,image_size_y=0,price_type=fixed,option_title=PE-Allo 594 50μg name=Conjugation,required=0,price=50.0000,type=drop_down,sku=,max_characters=0,file_extension=,image_size_x=0,image_size_y=0,price_type=fixed,option_title=PE-Cy5 50μg
created at :
5/8/14 15:19
updated at :
3/2/22 2:55
meta title :
白细胞介素-18抗体PB9014
meta keyword :
Anti-IL18 Antibody Picoband
meta description :
Boster Bio Anti-IL18 Antibody Picoband catalog # PB9014. Tested in ELISA, Flow Cytometry, IHC-P, WB applications. This antibody reacts with Mouse, Rat. Supplied as 100ug/vial in Lyophilized form antibody.
应用注释 :
Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.
rating value :
100
背景 :
Interleukin (IL)-18, also called Interferon-gamma-inducing factor (IGIF), augments natural killer (NK) activity in spleen cells. The gene encodes a precursor protein of 192 amino acids and a mature protein of 157 amino acids. IL18 is a recently discovered cytokine that modulates both T helper type 1 (Th1) and Th2 responses. IL18 is a potent proinflammatory cytokine with potential atherogenic properties. It is highly expressed in the atherosclerotic plaques compared with control normal arteries and is localized mainly in plaque macrophages.
competitor equivalent skus :
sc 10159 sc 10164 sc 28718 sc 136153 sc 136401 sc 398032
研究种类 :
Angiogenesis,Cancer,Cancer Metabolism,Cytokines,Immunology,Innate Immunity,Interleukins,Invasion/Microenvironment,Metabolism,Metabolism Processes,Pathways And Processes,Response To Hypoxia,Tumor Immunology
同义词 :
Interleukin-18;IL-18;Interferon gamma-inducing factor;IFN-gamma-inducing factor;Interleukin-1 gamma;IL-1 gamma;Il18;Igif;
基因全称 :
白细胞介素18
分子量 :
22303分子量
protein function :
Augments natural killer cell activity in spleen cells and stimulates interferon gamma production in T-helper type I cells.
subcellular localization :
Secreted.
蛋白名 :
白细胞介素18
recommended detection systems :
Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
template :
抗体
公司信息
武汉博士德生物工程有限公司
3942 B Valley Ave
Pleasanton, CA 94566
boster@bosterbio.com
http://www.boster.com.cn
925.485.4527
公司总部: 美国
Premium Provider of Antibodies and ELISA Kits