产品简要
公司名称 :
武汉博士德生物工程有限公司
产品类型 :
抗体
产品名称 :
Anti-ICAM1 Picoband Antibody
目录 :
PB9017
规格 :
100 ug/vial
价格 :
280美元
克隆性 :
多克隆
宿主 :
共轭标签 :
nonconjugated
反应物种 :
人类
应用 :
免疫印迹, 免疫细胞化学, 流式细胞仪, 免疫组化-石蜡切片, 免疫组化-冰冻切片
图像
图像 1 :
武汉博士德生物工程有限公司 PB9017 图像 1
Anti-ICAM1 Picoband antibody, PB9017-1.JPG. IHC(P): Human Intestinal Cancer Tissue
图像 2 :
武汉博士德生物工程有限公司 PB9017 图像 2
Anti-ICAM1 Picoband antibody, PB9017-2.jpg. All lanes: Anti-ICAM1(PB9017) at 0.5ug/ml. Lane 1: HEPG2 Whole Cell Lysate at 40ug. Lane 2: K562 Whole Cell Lysate at 40ug. Lane 3: A549 Whole Cell Lysate at 40ug. Lane 4: HT1080 Whole Cell Lysate at 40ug. Predicted bind size: 58KD. Observed bind size: 100KD
图像 3 :
武汉博士德生物工程有限公司 PB9017 图像 3
Figure 3. IF analysis of ICAM1 using anti-ICAM1 antibody (PB9017) . ICAM1 was detected in paraffin-embedded section of human tonsil tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/mL rabbit anti-ICAM1 Antibody (PB9017) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
产品信息
SKU号 :
PB9017
状态 :
Enabled
名称 :
Anti-ICAM1 Picoband Antibody
目录名称 :
一抗, 多克隆抗体, 免疫组化免疫细胞化学IF抗体
基因名称 :
细胞间粘附分子 1
价格 :
280美元
price various sizes :
Unconjugated / $280 APC / $330 APC-Cy7 / $330 FITC / $330 PE / $330 PE-Cy5 / $330 PE-Cy7 / $330 30ug sample size / $99 100ug / $280 100ug+Free HRP Secondary BA1054 / $280 100ug+Free Biotin Secondary BA1003 / $280
克隆性 :
多克隆
浓度 :
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
共轭标签 :
No
contents :
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
描述 :
Polyclonal antibody for CD54/ICAM1 detection. Host: Rabbit.Size: 100ug/vial. Tested applications: WB, IHC-P, IF. Reactive species: Human. CD54/ICAM1 information: Molecular Weight: 57825 MW; Subcellular Localization: Membrane; Single-pass type I membrane protein.
short description :
Rabbit IgG polyclonal antibody for Intercellular adhesion molecule 1(ICAM1) detection. Tested with WB, IHC-P, IHC-F, ICC, IF, FCM in Human.
规格 :
100 ug/vial
sample size available :
30ug for $99, contact us for details
UniProt数据库编号 :
P05362
宿主 :
免疫原 :
E.coli-derived human ICAM1 recombinant protein (Position: L214-P532). Human ICAM1 shares 52% and 48% amino acid (aa) sequences identity with mouse and rat ICAM1, respectively.
形式 :
冻干
纯化 :
免疫原亲和纯化
储存 :
At -20 C for one year. After reconstitution, at 4 C for one month. It can also be aliquotted and stored frozen at -20 C for a longer time.Avoid repeated freezing and thawing.
交叉活性 :
No cross reactivity with other proteins
重组 :
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
应用细节 :
Western blot, 0.1-0.5ug/ml, Human. Immunohistochemistry(Paraffin-embedded Section), 0.5-1ug/ml, Human, By Heat. Immunohistochemistry(Frozen Section), 0.5-1ug/ml, Human. Immunocytochemistry, 0.5-1ug/ml, Human. Immunofluorescence, 2ug/ml, Human. Flow Cytometry, 1-3ug/1x10 6 cells, Human
应用 :
流式细胞仪, IF, 免疫组化-P, 免疫组化-F, 免疫细胞化学, 免疫印迹
反应物种 :
人类
图像标记 :
Anti-ICAM1 Picoband antibody, PB9017-1.JPG. IHC(P): Human Intestinal Cancer Tissue Anti-ICAM1 Picoband antibody, PB9017-2.jpg. All lanes: Anti-ICAM1(PB9017) at 0.5ug/ml. Lane 1: HEPG2 Whole Cell Lysate at 40ug. Lane 2: K562 Whole Cell Lysate at 40ug. Lane 3: A549 Whole Cell Lysate at 40ug. Lane 4: HT1080 Whole Cell Lysate at 40ug. Predicted bind size: 58KD. Observed bind size: 100KD Figure 3. IF analysis of ICAM1 using anti-ICAM1 antibody (PB9017) . ICAM1 was detected in paraffin-embedded section of human tonsil tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/mL rabbit anti-ICAM1 Antibody (PB9017) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. Figure 4. IF analysis of ICAM1 using anti-ICAM1 antibody (PB9017) . ICAM1 was detected in paraffin-embedded section of human Lung cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/mL rabbit anti-ICAM1 Antibody (PB9017) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. Figure 5. Flow Cytometry analysis of A431 cells using anti-ICAM1 antibody (PB9017). Overlay histogram showing A431 cells stained with PB9017 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti- ICAM1 Antibody (PB9017,1ug/1x10 6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10ug/1x10 6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x10 6 ) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
背景 :
Intercellular adhesion molecule-1 (ICAM-1) is an integral membrane protein, a member of the immunoglobulin superfamily, and a ligand for lymphocyte function-associated (LFA) antigens, a beta 2 leukocyte integrin. The normal function of human ICAM-1 is to provide adhesion between endothelial cells and leukocytes after injury or stress. ICAM-1 binds to leukocyte function-associated antigen (LFA-1) or macrophage-1 antigen (Mac-1). It is found on leukocytes, fibroblasts, epithelial cells and endothelial cells and its expression is regulated by inflammatory cytokines. ICAM-1 has a tissue distribution similar to that of the major histocompatibility complex class II antigens and is likely to play a role in inflammatory responses.
competitor equivalent skus :
sc 101702 sc 107 sc 135946 sc 1511 sc 1511 R sc 18839 sc 18853 sc 18908 sc 31724 sc 31725 sc 366317 sc 390483 sc 53336 sc 71292 sc 7891 sc 8439
研究种类 :
Adaptive Immunity, Atherosclerosis, Cardiovascular, Cell Adhesion Molecules, Cell Type Markers, Hematopoietic Progenitors, Immunology, Lymphoid, Stem Cells, T Lymphocytic Lineage, Vascular Inflammation
同义词 :
Intercellular adhesion molecule 1;ICAM-1;Major group rhinovirus receptor;CD54;ICAM1;
基因全称 :
细胞间粘附分子1
分子量 :
57825分子量
protein function :
ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans- endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation. In case of rhinovirus infection acts as a cellular receptor for the virus.
subcellular localization :
Membrane; Single-pass type I membrane protein.
蛋白名 :
细胞间粘附分子1
recommended detection systems :
Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P), IHC(F) and ICC.
sequence similarities :
Belongs to the immunoglobulin superfamily. ICAM family.
更新 :
3/29/19 8:40
公司信息
武汉博士德生物工程有限公司
3942 B Valley Ave
Pleasanton, CA 94566
boster@bosterbio.com
http://www.boster.com.cn
925.485.4527
公司总部: 美国
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