产品简要
公司名称 :
武汉博士德生物工程有限公司
产品类型 :
抗体
产品名称 :
Anti-Serum Amyloid P/APCS Picoband Antibody
目录 :
PB9021
规格 :
100 ug/vial
价格 :
280美元
克隆性 :
多克隆
宿主 :
共轭标签 :
nonconjugated
反应物种 :
小鼠, 大鼠
应用 :
免疫印迹, 免疫组化-石蜡切片
图像
图像 1 :
武汉博士德生物工程有限公司 PB9021 图像 1
Figure 1. IHC analysis of Serum Amyloid P using anti-Serum Amyloid P antibody (PB9021). Serum Amyloid P was detected in paraffin-embedded section of rat liver tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-Serum Amyloid P Antibody (PB9021) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
图像 2 :
武汉博士德生物工程有限公司 PB9021 图像 2
Figure 2. Western blot analysis of Serum Amyloid P using anti-Serum Amyloid P antibody (PB9021). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, . Lane 2: mouse liver tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Serum Amyloid P antigen affinity purified polyclonal antibody (Catalog # PB9021) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Serum Amyloid P at approximately 29KD. The expected band size for Serum Amyloid P is at 25KD.
产品信息
SKU号 :
PB9021
状态 :
Enabled
名称 :
Anti-Serum Amyloid P/APCS Picoband Antibody
目录名称 :
一抗, 多克隆抗体, 免疫组化免疫细胞化学IF抗体
基因名称 :
APCS
价格 :
280美元
price various sizes :
Unconjugated / $280 APC / $330 APC-Cy7 / $330 FITC / $330 PE / $330 PE-Cy5 / $330 PE-Cy7 / $330 30ug sample size / $99 100ug / $280 100ug+Free HRP Secondary BA1054 / $280 100ug+Free Biotin Secondary BA1003 / $280
克隆性 :
多克隆
浓度 :
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
共轭标签 :
No
contents :
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
描述 :
Polyclonal antibody for SAP/APCS detection. Host: Rabbit.Size: 100ug/vial. Tested applications: IHC-P. Reactive species: Mouse, Rat. SAP/APCS information: Molecular Weight: 26176 MW; Subcellular Localization: Secreted.
short description :
Rabbit IgG polyclonal antibody for Serum amyloid P-component(APCS) detection. Tested with WB, IHC-P in Mouse;Rat.
规格 :
100 ug/vial
sample size available :
30ug for $99, contact us for details
UniProt数据库编号 :
P23680
宿主 :
免疫原 :
E.coli-derived rat Serum Amyloid P recombinant protein (Position: Q21-S228). Rat Serum Amyloid P shares 72% and 80% amino acid (aa) sequences identity with human and mouse Serum Amyloid P, respectively.
形式 :
冻干
纯化 :
免疫原亲和纯化
储存 :
At -20 C for one year. After reconstitution, at 4 C for one month. It can also be aliquotted and stored frozen at -20 C for a longer time.Avoid repeated freezing and thawing.
交叉活性 :
No cross reactivity with other proteins
重组 :
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
应用细节 :
Western blot, 0.1-0.5ug/ml, Mouse, Rat. Immunohistochemistry(Paraffin-embedded Section), 0.5-1ug/ml, Rat, By Heat.
应用 :
免疫组化-P, 免疫印迹
反应物种 :
小鼠, 大鼠
图像标记 :
Figure 1. IHC analysis of Serum Amyloid P using anti-Serum Amyloid P antibody (PB9021). Serum Amyloid P was detected in paraffin-embedded section of rat liver tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-Serum Amyloid P Antibody (PB9021) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. Figure 2. Western blot analysis of Serum Amyloid P using anti-Serum Amyloid P antibody (PB9021). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, . Lane 2: mouse liver tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Serum Amyloid P antigen affinity purified polyclonal antibody (Catalog # PB9021) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Serum Amyloid P at approximately 29KD. The expected band size for Serum Amyloid P is at 25KD.
背景 :
Amyloid P component, serum(SAP), also known as PTX2 or APCS, is the identical serum form of amyloid P component(AP), a 25kDa pentameric protein first identified as the pentagonal constituent of in vivo pathological deposits called "amyloid". It belongs to the pentraxins family, characterised by calcium dependent ligand binding and distinctive flattened beta-jellyroll structure similar to that of the legume lectins. This gene is mapped to 1q23.2. The binding of the encoded protein to proteins in the pathological amyloid cross-beta fold suggests its possible role as a chaperone. This protein is also thought to control the degradation of chromatin. It has been demonstrated that this protein binds to apoptotic cells at an early stage, which raises the possibility that it is involved in dealing with apoptotic cells in vivo.
competitor equivalent skus :
sc 100528 sc 135069 sc 138078 sc 138079 sc 15803 sc 15804 sc 163950 sc 18309 sc 18310 sc 245912 sc 245913 sc 246555 sc 246556 sc 246557 sc 292686 sc 292687 sc 292972 sc 368736 sc 374654 sc 377175 sc 393704 sc 393948 sc 52718 sc 52719 sc 53165 sc 53166 sc 53647 sc 54538 sc 54539 sc 59652 sc 65398 sc 65958 sc 65959 sc 69662 sc 69796 sc 71094 sc 7930 sc 80504 sc 81682 sc 81683 sc 82714 sc 84112 sc 84113 sc 895 sc 896 sc 9998
同义词 :
Serum amyloid P-component;SAP;Apcs;Ptx2, Sap;
基因全称 :
血清淀粉样蛋白P成分
分子量 :
26176分子量
subcellular localization :
Secreted.
蛋白名 :
血清淀粉样蛋白P成分
recommended detection systems :
Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
sequence similarities :
Belongs to the pentaxin family.
更新 :
4/18/19 2:40
公司信息
武汉博士德生物工程有限公司
3942 B Valley Ave
Pleasanton, CA 94566
boster@bosterbio.com
http://www.boster.com.cn
925.485.4527
公司总部: 美国
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