产品简要
公司名称 :
武汉博士德生物工程有限公司
产品类型 :
抗体
产品名称 :
Anti-Ki67/MKI67 Antibody Picoband™
目录 :
PB9026
规格 :
100μg/vial
价格 :
315美元
克隆性 :
多克隆
宿主 :
domestic rabbit
共轭标签 :
未共轭
反应物种 :
人类
应用 :
免疫印迹, 免疫细胞化学, 免疫组化-石蜡切片
图像
图像 1 :
武汉博士德生物工程有限公司 PB9026 图像 1
Anti-Ki67 Picoband antibody, PB9026-1.JPG. IHC(P): Human Tonsil Tissue
图像 2 :
武汉博士德生物工程有限公司 PB9026 图像 2
Anti-Ki67 Picoband antibody, PB9026-2.jpg. All lanes: Anti-Ki67(PB9026) at 0.5ug/ml. Lane 1: HELA Whole Cell Lysate at 40ug. Lane 2: MCF-7 Whole Cell Lysate at 40ug . Lane 3: COLO320 Whole Cell Lysate at 40ug. Lane 4: HEPG2 Whole Cell Lysate at 40ug. Lane 5: SKOV Whole Cell Lysate at 40ug. Predicted bind size: 358KD. Observed bind size: 358KD
产品信息
SKU号 :
PB9026
产品名称 :
Anti-Ki67/MKI67 Antibody Picoband™
价格 :
315美元
规格 :
100μg/vial
克隆性 :
多克隆
宿主 :
反应物种 :
人类
应用(s) :
IF, 免疫组化-P, 免疫细胞化学, 免疫印迹
应用细节 :
Western blot, 0.1-0.5µg/ml, Human. Immunohistochemistry (Paraffin-embedded Section), 0.5-1µg/ml, Human, By Heat. Immunocytochemistry , 0.5-1µg/ml, Human. Immunofluorescence, 5µg/ml, Human.
应用注释 :
WB: The detection limit for Ki67 is approximately 0.25ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.
描述 :
Boster Bio Anti-Ki67/MKI67 Antibody Picoband™ catalog # PB9026. Tested in IF, IHC-P, ICC, WB applications. This antibody reacts with Human.
浓度 :
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
基因名称 :
MKI67
UniProt数据库编号 :
P46013
免疫原 :
E.coli-derived human Ki67 recombinant protein (Position: K2860-I3256).
形式 :
冻干
Contents :
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
纯化 :
免疫原亲和纯化
交叉活性 :
No cross reactivity with other proteins
储存 :
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
重组 :
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
基因全称 :
抗原KI-67
同义词 :
抗原KI-67;MKI67;
蛋白名 :
抗原KI-67
分子量 :
358694分子量
Protein Function :
Thought to be required for maintaining cell proliferation.
Subcellular Localization :
Nucleus. Nucleus, nucleolus. Chromosome. Predominantly localized in the G1 phase in the perinucleolar region, in the later phases it is also detected throughout the nuclear interior, being predominantly localized in the nuclear matrix. In mitosis, it is present on all chromosomes.
Recommended Detection Systems :
Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P) and ICC.
Sequence Similarities :
含1 FHA区域
背景 :
Ki-67 (Proliferation-related Ki-67 antigen), also known as MKI67 or KIA, is a protein that in humans is encoded by the MKI67 gene. From study of a panel of human-rodent somatic cell hybrids, it has been demonstrated that a gene involved in the expression of the MKI67 antigen is located on chromosome 10. By in situ hybridization, Fonatsch et al. (1991) regionalized the MKI67 gene to chromosome 10q25-qter. By FISH, Traut et al. (1998) mapped the mouse Mki67 gene to chromosome 7F3-F5. Antigen KI-67 is a nuclear protein that is associated with and may be necessary for cellular proliferation. Furthermore it is associated with ribosomal RNA transcription. Inactivation of antigen KI-67 leads to inhibition of ribosomal RNA synthesis.
研究种类 :
Cancer, Cell Biology, Cell Cycle, Cell Division, Cell Type Marker, Cell Type Markers, Neurogenesis, Neurology Process, Neuron Marker, Neuroscience, Tags & Cell Markers, Tumor Biomarkers
公司信息
武汉博士德生物工程有限公司
3942 B Valley Ave
Pleasanton, CA 94566
boster@bosterbio.com
http://www.boster.com.cn
925.485.4527
公司总部: 美国
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