抗体

Anti-Csk Antibody Picoband™

PB9102

100μg/vial

315美元

多克隆

domestic rabbit

未共轭

人类, 小鼠, 大鼠

免疫印迹, 免疫组化-石蜡切片

Anti-Csk Antibody Picoband™

315美元

100μg/vial

多克隆

兔

人类, 小鼠, 大鼠

免疫组化-P, 免疫印迹

Immunohistochemistry (Paraffin-embedded Section), 0.5-1µg/ml, Human, By Heat. Western blot, 0.1-0.5µg/ml, Human, Mouse, Rat.

WB: The detection limit for CSK is approximately 0.25ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Boster Bio Anti-Csk Antibody Picoband™ catalog # PB9102. Tested in IHC-P, WB applications. This antibody reacts with Human, Mouse, Rat.

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

CSK

P41240

E.coli-derived human CSK recombinant protein (Position: S2-G204). Human CSK shares 99% amino acid (aa) sequence identity with both mouse and rat CSK.

冻干

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

免疫原亲和纯化

No cross reactivity with other proteins

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

酪氨酸蛋白激酶CSK

Tyrosine-protein kinase CSK; 2.7.10.2; C-Src kinase; Protein-tyrosine kinase CYL; CSK;

酪氨酸蛋白激酶CSK

50704分子量

Non-receptor tyrosine-protein kinase that plays an important role in the regulation of cell growth, differentiation, migration and immune response. Phosphorylates tyrosine residues located in the C-terminal tails of Src-family kinases (SFKs) including LCK, SRC, HCK, FYN, LYN or YES1. Upon tail phosphorylation, Src-family members engage in intramolecular interactions between the phosphotyrosine tail and the SH2 domain that result in an inactive conformation. To inhibit SFKs, CSK is recruited to the plasma membrane via binding to transmembrane proteins or adapter proteins located near the plasma membrane. Suppresses signaling by various surface receptors, including T- cell receptor (TCR) and B-cell receptor (BCR) by phosphorylating and maintaining inactive several positive effectors such as FYN or LCK.

Cytoplasm. Cell membrane. Mainly cytoplasmic, also present in lipid rafts.

Expressed in lung and macrophages.

Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).

Belongs to the protein kinase superfamily. Tyr protein kinase family. CSK subfamily.

CSK also known as C-Src kinase, is a non-receptor protein tyrosine kinase protein that in humans is encoded by the CSK gene. It is mapped to 15q24.1. CSK suppresses activity of the Src family of protein kinases by phosphorylation of Src family members at a conserved C-terminal site in Src. Csk's control of the Src family activity is widely thought to be central to regulation of the immune response. CSK can downregulate tyrosine kinase activity of the SRC oncoprotein through tyrosine phosphorylation of the SRC carboxy terminus. Since cell transformation by SRC oncoproteins is caused by various mechanisms that interfere with this phosphorylation, the CSK gene might function as an antioncogene.

蛋白磷酸化, 信号转导, 酪氨酸激酶