产品简要
公司名称 :
武汉博士德生物工程有限公司
产品类型 :
抗体
产品名称 :
Anti-MCL1 Antibody Picoband™
目录 :
PB9132
规格 :
100μg/vial
价格 :
315美元
克隆性 :
多克隆
宿主 :
domestic rabbit
共轭标签 :
未共轭
反应物种 :
人类, 小鼠, 大鼠
应用 :
免疫印迹, 免疫组化, 免疫细胞化学, 免疫沉淀
文章摘录数: 1
出版应用/物种/样本/稀释参考文献
  • 免疫沉淀; 人类; 图 1c
  • 免疫细胞化学; 人类; 图 2a
  • 免疫印迹; 人类; 图 s1a
  • 免疫细胞化学; 小鼠; 图 7a
  • 免疫印迹; 小鼠; 图 7g
Nikhil K, Shah K. The Cdk5-Mcl-1 axis promotes mitochondrial dysfunction and neurodegeneration in a model of Alzheimer's disease. J Cell Sci. 2017;130:3023-3039 pubmed 出版商
图像
图像 1 :
武汉博士德生物工程有限公司 PB9132 图像 1
Anti-MCL1 Picoband antibody, PB9132-1.jpg. All lanes: Anti MCL1 (PB9132) at 0.5ug/ml. WB: Recombinant Human MCL1 Protein 0.5ng. Predicted bind size: 40KD. Observed bind size: 40KD
图像 2 :
武汉博士德生物工程有限公司 PB9132 图像 2
Anti-MCL1 Picoband antibody, PB9132-2.jpg. All lanes: Anti MCL1 (PB9132) at 0.5ug/ml. Lane 1: Rat Spleen Tissue Lysate at 50ug. Lane 2: HEPG2 Whole Cell Lysate at 40ug. Lane 3: MCF-7 Whole Cell Lysate at 40ug. Lane 4: COLO320 Whole Cell Lysate at 40ug. Predicted bind size: 37KD . Observed bind size: 37KD
图像 3 :
武汉博士德生物工程有限公司 PB9132 图像 3
Anti-MCL1 Picoband antibody, PB9132-3.JPG. IHC(P): Rat Intestine Tissue
产品信息
SKU号 :
PB9132
产品名称 :
Anti-MCL1 Antibody Picoband™
价格 :
315美元
规格 :
100μg/vial
克隆性 :
多克隆
宿主 :
反应物种 :
人类, 小鼠, 大鼠
应用(s) :
免疫组化, 免疫印迹
应用细节 :
Immunohistochemistry (Paraffin-embedded Section), 0.5-1µg/ml, Human, Mouse, Rat, By Heat. Western blot, 0.1-0.5µg/ml, Human, Rat.
应用注释 :
WB: The detection limit for MCL1 is approximately 0.25ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.
描述 :
Boster Bio Anti-MCL1 Antibody Picoband™ catalog # PB9132. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.
浓度 :
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
基因名称 :
髓细胞白血病-1基因
UniProt数据库编号 :
Q07820
免疫原 :
E.coli-derived human MCL1 recombinant protein (Position: M1-R350). Human MCL1 shares 82% and 78% amino acid (aa) sequences identity with mouse and rat MCL1, respectively.
形式 :
冻干
Contents :
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
纯化 :
免疫原亲和纯化
交叉活性 :
No cross reactivity with other proteins
储存 :
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
重组 :
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
基因全称 :
Induced myeloid leukemia cell differentiation protein Mcl-1
同义词 :
Induced myeloid leukemia cell differentiation protein Mcl-1; Bcl-2-like protein 3; Bcl2-L-3; Bcl-2-related protein EAT/mcl1; mcl1/EAT; MCL1; BCL2L3;
蛋白名 :
Induced myeloid leukemia cell differentiation protein Mcl-1
分子量 :
37337分子量
Protein Function :
Involved in the regulation of apoptosis versus cell survival, and in the maintenance of viability but not of proliferation. Mediates its effects by interactions with a number of other regulators of apoptosis. Isoform 1 inhibits apoptosis. Isoform 2 promotes apoptosis.
Subcellular Localization :
Membrane ; Single-pass membrane protein. Cytoplasm. Mitochondrion. Nucleus, nucleoplasm. Cytoplasmic, associated with mitochondria.
Recommended Detection Systems :
Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
Sequence Similarities :
Belongs to the Bcl-2 family.
背景 :
MCL1, also known as myeloid cell leukemia 1, is a protein that in humans is encoded the MCL1 gene. It is mapped to 1q21.3. MCL1 is a potent multidomain antiapoptotic protein of the BCL2 family that heterodimerizes with other BCL2 family members to protect against apoptotic cell death. MCL1 as an attractive candidate for regulation of hematopoietic stem cell homeostasis that is highly expressed in hematopoietic stem cells and regulated by growth factor signals. MCL1 is a critical and specific regulator essential for ensuring the homeostasis of early hematopoietic progenitors. During mitotic arrest, MCL1 protein levels decline markedly, through a posttranslational mechanism, potentiating cell death. Phosphorylation of MCL1 directs its interaction with the tumor suppressor protein FBW7, which is the substrate-binding component of a ubiquitin ligase complex.
研究种类 :
Apoptosis, Apoptotic Markers, Cancer, Cell Biology, Cell Death, Intracellular, Invasion/Microenvironment, Metabolism, Metabolism Processes, Pathways And Processes
公司信息
武汉博士德生物工程有限公司
3942 B Valley Ave
Pleasanton, CA 94566
boster@bosterbio.com
http://www.boster.com.cn
925.485.4527
公司总部: 美国
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