产品简要
公司名称 :
武汉博士德生物工程有限公司
产品类型 :
抗体
产品名称 :
Anti-MyD88 PTGS2 Antibody Picoband™
目录 :
PB9148
规格 :
100μg/vial
价格 :
315美元
克隆性 :
多克隆
宿主 :
domestic rabbit
共轭标签 :
未共轭
反应物种 :
人类, 小鼠, 大鼠
应用 :
免疫印迹, 酶联免疫吸附测定, 免疫细胞化学, 流式细胞仪, 免疫组化-石蜡切片, 免疫组化-冰冻切片
更多信息或购买 :
武汉博士德生物工程有限公司 产品网页
图像
图像 1 :
武汉博士德生物工程有限公司 PB9148 图像 1
Figure 1. Western blot analysis of MYD88 using anti-MYD88 antibody (PB9148). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Recombinant Human MYD88 Protein 0.5ng. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MYD88 antigen affinity purified polyclonal antibody (Catalog # PB9148) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MYD88 at approximately 49KD. The expected band size for MYD88 is at 49KD.
图像 2 :
武汉博士德生物工程有限公司 PB9148 图像 2
Figure 2. Western blot analysis of MYD88 using anti-MYD88 antibody (PB9148). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: Rat Cardiac Muscle Tissue Lysate. Lane 2: HELA Whole Cell Lysate. Lane 3: MCF Whole Cell Lysate. Lane 4: HEPG2 Whole Cell Lysate. Lane 5: JURKAT Whole Cell Lysate. Lane 6: RAJI Whole Cell Lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MYD88 antigen affinity purified polyclonal antibody (Catalog # PB9148) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MYD88 at approximately 33KD. The expected band size for MYD88 is at 33KD.
图像 3 :
武汉博士德生物工程有限公司 PB9148 图像 3
Figure 3. IHC analysis of MYD88 using anti-MYD88 antibody (PB9148). MYD88 was detected in paraffin-embedded section of rat lung tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-MYD88 Antibody (PB9148) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
产品信息
SKU号 :
PB9148
产品名称 :
Anti-MyD88 PTGS2 Antibody Picoband™
价格 :
315美元
规格 :
100μg/vial
克隆性 :
多克隆
宿主 :
兔
反应物种 :
人类, 小鼠, 大鼠
应用(s) :
酶联免疫吸附测定, 流式细胞仪, IF, 免疫组化-P, 免疫组化-F, 免疫细胞化学, 免疫印迹
应用细节 :
Western blot, 0.1-0.5µg/ml. Immunohistochemistry (Paraffin-embedded Section), 0.5-1µg/ml. Immunohistochemistry (Frozen Section), 0.5-1µg/ml. Immunocytochemistry, 0.5-1µg/ml. Immunofluorescence, 2µg/ml. Flow Cytometry, 1-3μg/1x10^6 cells. ELISA (Cap), 1-5µg/ml.
应用注释 :
WB: The detection limit for MyD88 is approximately 0.25ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.
描述 :
Boster Bio Anti-MyD88 PTGS2 Antibody Picoband™ catalog # PB9148. Tested in ELISA, Flow Cytometry, IF, IHC-P, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.
浓度 :
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
基因名称 :
MYD88
UniProt数据库编号 :
P35354
免疫原 :
E.coli-derived human MyD88 recombinant protein (Position: A44-F264). Human MyD88 shares 84% and 83% amino acid (aa) sequences identity with mouse and rat MyD88, respectively.
形式 :
冻干
Contents :
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
纯化 :
免疫原亲和纯化
交叉活性 :
No cross reactivity with other proteins
储存 :
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
重组 :
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
基因全称 :
Prostaglandin G/H synthase 2
同义词 :
Prostaglandin G/H synthase 2; 1.14.99.1; Cyclooxygenase-2; COX-2; PHS II; Prostaglandin H2 synthase 2; PGH synthase 2; PGHS-2; Prostaglandin-endoperoxide synthase 2; PTGS2; COX2;
蛋白名 :
Myeloid differentiation primary response protein MyD88
分子量 :
68996分子量
Protein Function :
Converts arachidonate to prostaglandin H2 (PGH2), a committed step in prostanoid synthesis. Constitutively expressed in some tissues in physiological conditions, such as the endothelium, kidney and brain, and in pathological conditions, such as in cancer. PTGS2 is responsible for production of inflammatory prostaglandins. Up-regulation of PTGS2 is also associated with increased cell adhesion, phenotypic changes, resistance to apoptosis and tumor angiogenesis. In cancer cells, PTGS2 is a key step in the production of prostaglandin E2 (PGE2), which plays important roles in modulating motility, proliferation and resistance to apoptosis.
Subcellular Localization :
Microsome membrane; Peripheral membrane protein. Endoplasmic reticulum membrane; Peripheral membrane protein.
组织特异性 :
Ubiquitous.
Recommended Detection Systems :
Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P), IHC(F) and ICC.
Sequence Similarities :
含1死亡区域
背景 :
MYD88 (MYELOID DIFFERENTIATION PRIMARY RESPONSE GENE 88), is a protein that, in humans, is encoded by the MYD88 gene. MyD88 is a key downstream adapter for most Toll-like receptors (TLRs) and interleukin-1 receptors (IL1Rs). And it is mapped on 3p22.2. MYD88 encodes a cytosolic adapter protein that plays a central role in the innate and adaptive immune response. This protein functions as an essential signal transducer in the interleukin-1 and Toll-like receptor signaling pathways. Overexpression of MYD88 caused an increase in the level of transcription from the interleukin-8 promoter. The C-terminal domain of MYD88 has significant sequence similarity to the cytoplasmic domain of IL1RAP. Inhibiting the IL1R-MYD88 pathway in vivo could block the damage from acute inflammation that occurs in response to sterile cell death, and do so in a way that might not compromise tissue repair or host defense against pathogens.
研究种类 :
Atherosclerosis, Cancer, Cancer Metabolism, Cardiovascular, Cholesterol Metabolism, Endocrine Metabolism, Heart Disease, Hormone Biosynthesis, Lipid And Lipoprotein Metabolism, Lipid Metabolism, Lipid Signaling, Lipids / Lipoproteins, Metabolic Signaling Pathway, Metabolic Signaling Pathways, Metabolism, Pathways And Processes, Platelets, Signal Transduction, Signaling Pathway, Tumor Biomarkers
更多信息或购买 :
武汉博士德生物工程有限公司 产品网页
公司信息
武汉博士德生物工程有限公司
3942 B Valley Ave
Pleasanton, CA 94566
boster@bosterbio.com
http://www.boster.com.cn
925.485.4527
公司总部: 美国
Premium Provider of Antibodies and ELISA Kits