产品简要
公司名称 :
武汉博士德生物工程有限公司
产品类型 :
抗体
产品名称 :
Anti-NFkB p105/p50/NFKB1 Antibody Picoband™
目录 :
PB9149
规格 :
100μg/vial
价格 :
315美元
克隆性 :
多克隆
宿主 :
domestic rabbit
共轭标签 :
未共轭
反应物种 :
人类, 大鼠
应用 :
免疫印迹, 免疫组化, 免疫组化-石蜡切片
文章摘录数: 2
出版应用/物种/样本/稀释参考文献
  • 免疫组化-石蜡切片; 人类; 图 8
Nazmeen A, Chen G, Ghosh T, Maiti S. Breast cancer pathogenesis is linked to the intra-tumoral estrogen sulfotransferase (hSULT1E1) expressions regulated by cellular redox dependent Nrf-2/NFκβ interplay. Cancer Cell Int. 2020;20:70 pubmed 出版商
Fiore A, Ugel S, De Sanctis F, Sandri S, Fracasso G, Trovato R, et al. Induction of immunosuppressive functions and NF-κB by FLIP in monocytes. Nat Commun. 2018;9:5193 pubmed 出版商
图像
图像 1 :
武汉博士德生物工程有限公司 PB9149 图像 1
Anti-NFkB p105/P50 Picoband antibody, PB9149-1.jpg. All lanes: Anti NFKBP105 (PB9149) at 0.5ug/ml. WB: Recombinant Human NFKBP105 Protein 0.5ng. Predicted bind size: 40KD. Observed bind size: 40KD
图像 2 :
武汉博士德生物工程有限公司 PB9149 图像 2
Anti-NFkB p105/P50 Picoband antibody, PB9149-2.jpg. All lanes: Anti NFKBP105 (PB9149) at 0.5ug/ml. Lane 1: Rat Spleen Tissue Lysate at 50ug. Lane 2: PC-12 Whole Cell Lysate at 40ug. Lane 3: HELA Whole Cell Lysate at 40ug. Lane 4: A431 Whole Cell Lysate at 40ug. Lane 5: JURKAT Whole Cell Lysate at 40ug. Lane 6: MCF-7 Whole Cell Lysate at 40ug. Predicted bind size: 50KD . Observed bind size: 50KD
图像 3 :
武汉博士德生物工程有限公司 PB9149 图像 3
Anti-NFkB p105/P50 Picoband antibody, PB9149-3.JPG. IHC(P): Human Lung Cancer Tissue
产品信息
SKU号 :
PB9149
产品名称 :
Anti-NFkB p105/p50/NFKB1 Antibody Picoband™
价格 :
315美元
规格 :
100μg/vial
克隆性 :
多克隆
宿主 :
反应物种 :
人类, 大鼠
应用(s) :
免疫组化, 免疫印迹
应用细节 :
Immunohistochemistry (Paraffin-embedded Section), 0.5-1µg/ml, Human, By Heat. Western blot, 0.1-0.5µg/ml, Human, Rat.
应用注释 :
WB: The detection limit for NFkB p105/P50 is approximately 0.25ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.
描述 :
Boster Bio Anti-NFkB p105/p50/NFKB1 Antibody Picoband™ catalog # PB9149. Tested in IHC, WB applications. This antibody reacts with Human, Rat.
浓度 :
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
基因名称 :
NFKB1
UniProt数据库编号 :
P19838
免疫原 :
E.coli-derived human NFkB p105/P50 recombinant protein (Position: M1-Q360). Human NFkB p105/P50 shares 93% amino acid (aa) sequence identity with mouse NFkB p105/P50.
形式 :
冻干
Contents :
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
纯化 :
免疫原亲和纯化
交叉活性 :
No cross reactivity with other proteins
储存 :
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
重组 :
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
基因全称 :
Nuclear factor NF-kappa-B p105 subunit
同义词 :
Nuclear factor NF-kappa-B p105 subunit; DNA-binding factor KBF1; EBP-1; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 1; Nuclear factor NF-kappa-B p50 subunit; NFKB1;
蛋白名 :
Nuclear factor NF-kappa-B p105 subunit
分子量 :
105356分子量
Protein Function :
NF-kappa-B is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52 and the heterodimeric p65-p50 complex appears to be most abundant one. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. NF-kappa-B heterodimeric p65-p50 and RelB-p50 complexes are transcriptional activators. The NF-kappa-B p50-p50 homodimer is a transcriptional repressor, but can act as a transcriptional activator when associated with BCL3. NFKB1 appears to have dual functions such as cytoplasmic retention of attached NF-kappa-B proteins by p105 and generation of p50 by a cotranslational processing. The proteasome-mediated process ensures the production of both p50 and p105 and preserves their independent function, although processing of NFKB1/p105 also appears to occur post-translationally. p50 binds to the kappa-B consensus sequence 5'-GGRNNYYCC-3', located in the enhancer region of genes involved in immune response and acute phase reactions. In a complex with MAP3K8, NFKB1/p105 represses MAP3K8-induced MAPK signaling; active MAP3K8 is released by proteasome-dependent degradation of NFKB1/p105.
Subcellular Localization :
Nucleus. Cytoplasm. Nuclear, but also found in the cytoplasm in an inactive form complexed to an inhibitor (I-kappa-B).
Recommended Detection Systems :
Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
Sequence Similarities :
Contains 7 ANK repeats.
背景 :
Nuclear factor NF-kappa-B p105 subunit, also called EBP-1 is a protein that in humans is encoded by the NFKB1 gene. By fluorescence in situ hybridization, the gene was assigned to human chromosome 4q24. NFKB1 is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NFKB1 appears to have dual functions such as cytoplasmic retention of attached NFKB1 proteins by p105 and generation of p50 by a cotranslational processing.
研究种类 :
Apoptosis, Apoptotic Markers, Cancer, Cell Biology, Cell Death, Epigenetics and Nuclear Signaling, Immunology, Innate Immunity, Intracellular, Macrophage / Inflamm., Nfkb Pathway, Nuclear Signaling, Nuclear Signaling Pathways, Signal Transduction, Signaling Pathway, Transcription, Transcription Factors
公司信息
武汉博士德生物工程有限公司
3942 B Valley Ave
Pleasanton, CA 94566
boster@bosterbio.com
http://www.boster.com.cn
925.485.4527
公司总部: 美国
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