抗体

Anti-PD-L1/CD274 Antibody Picoband™

PB9154

100μg/vial

315美元

多克隆

domestic rabbit

未共轭

免疫印迹, 流式细胞仪

Anti-PD-L1/CD274 Antibody Picoband™

315美元

100μg/vial

多克隆

兔

人类

流式细胞仪, 免疫印迹

免疫印迹, 0.25 0.5µg/毫升, 人类。 流式细胞仪, 1-3微克/1x10^6细胞, 人类

WB: The detection limit for PD-L1 is approximately 0.25ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Boster Bio Anti-PD-L1/CD274 Antibody Picoband™ catalog # PB9154. Tested in Flow Cytometry, WB applications. This antibody reacts with Human.

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

CD274

Q9NZQ7

E.coli-derived human PD-L1 recombinant protein (Position: E45-T290). Human PD-L1 shares 69% amino acid (aa) sequence identity with mouse PD-L1.

冻干

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

免疫原亲和纯化

No cross reactivity with other proteins

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Programmed cell death 1 ligand 1

Programmed cell death 1 ligand 1; PD-L1; PDCD1 ligand 1; Programmed death ligand 1; B7 homolog 1; B7-H1; CD274; CD274; B7H1, PDCD1L1, PDCD1LG1, PDL1;

Programmed cell death 1 ligand 1

33275分子量

Involved in the costimulatory signal, essential for T- cell proliferation and production of IL10 and IFNG, in an IL2- dependent and a PDCD1-independent manner. Interaction with PDCD1 inhibits T-cell proliferation and cytokine production.

Isoform 1: Cell membrane; Single-pass type I membrane protein.

Highly expressed in the heart, skeletal muscle, placenta and lung. Weakly expressed in the thymus, spleen, kidney and liver. Expressed on activated T- and B-cells, dendritic cells, keratinocytes and monocytes.

Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.

Belongs to the immunoglobulin superfamily. BTN/MOG family.

Programmed death-ligand 1 (PD-L1) also known as CD274 or B7-H1 is a protein that in humans is encoded by the CD274 gene. It is mapped to 9p24.1. PD-L1 is a 40kDa type 1 transmembrane protein that has been speculated to play a major role in suppressing the immune system during particular events such as pregnancy, tissue allografts, autoimmune disease and other disease states such as hepatitis. It has been concluded that upregulation of PD-L1 on tumor MDCs downregulates T-cell immunity and that PD-L1 blockade may represent an approach for cancer immunotherapy. PD-L1 can provide positive costimulatory signals for innate and adaptive immunity and for protection against intracellular bacterial infection. What’s more, It has been found that PD1/PDL1 pathway may be a good target for restoring antitumor immunity in ovarian cancer.

Autoimmune, Cancer, Cell Type Markers, Immune System Diseases, Immunology, Tumor Antigens, Tumor Biomarkers, Tumor Immunology, Tumor-Associated Antigens