抗体

Anti-B Raf/BRAF Antibody Picoband™

PB9164

100μg/vial

315美元

多克隆

domestic rabbit

未共轭

人类, 小鼠, 大鼠

免疫印迹, 流式细胞仪, 免疫组化-石蜡切片

Anti-B Raf/BRAF Antibody Picoband™

315美元

100μg/vial

多克隆

兔

人类, 小鼠, 大鼠

流式细胞仪, 免疫组化-P, 免疫印迹

免疫印迹, 0.1 0.5µg/毫升, 人类, 小鼠, 大鼠。 免疫组化(石蜡包埋切片), 2-5微克/毫升, 人类, 小鼠, 大鼠。 流式细胞仪, 1-3微克/1x10^6细胞, 人类

WB: The detection limit for B Raf is approximately 0.25ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Boster Bio Anti-B Raf/BRAF Antibody Picoband™ catalog # PB9164. Tested in Flow Cytometry, IHC-P, WB applications. This antibody reacts with Human, Mouse, Rat.

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

BRAF

P15056

E.coli-derived human B Raf recombinant protein (Position: A38-V230). Human B Raf shares 81% amino acid (aa) sequence identity with mouse B Raf.

冻干

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

免疫原亲和纯化

No cross reactivity with other proteins

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Serine/threonine-protein kinase B-raf

Serine/threonine-protein kinase B-raf; 2.7.11.1; Proto-oncogene B-Raf; p94; v-Raf murine sarcoma viral oncogene homolog B1; BRAF; BRAF1, RAFB1;

Serine/threonine-protein kinase B-raf

84437分子量

Protein kinase involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May play a role in the postsynaptic responses of hippocampal neuron. Phosphorylates MAP2K1, and thereby contributes to the MAP kinase signal transduction pathway.

Nucleus. Cytoplasm. Cell membrane. Colocalizes with RGS14 and RAF1 in both the cytoplasm and membranes.

大脑和睾丸

Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).

Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. RAF subfamily.

BRAF (v-raf murine sarcoma viral oncogene homolog B1) is a human gene that makes a protein called B-Raf. It is a member of the Raf kinase family of growth signal transduction protein kinases. This protein plays a role in regulating the MAP kinase/ERKs signaling pathway, which affects cell division, differentiation, and secretion. It is mapped to 7q34. Mutations in this gene are associated with cardiofaciocutaneous syndrome, a disease characterized by heart defects, mental retardation and a distinctive facial appearance. The BRAF protein is also involved in sending signals inside cells, which are involved in directing cell growth.