产品简要
公司名称 :
武汉博士德生物工程有限公司
产品类型 :
抗体
产品名称 :
Anti-FGFR4 Antibody Picoband™
目录 :
PB9193
规格 :
100μg/vial
价格 :
315美元
克隆性 :
多克隆
宿主 :
domestic rabbit
共轭标签 :
未共轭
反应物种 :
人类
应用 :
免疫印迹, 免疫组化
更多信息或购买 :
图像
图像 1 :
Anti- FGFR4 antibody, PB9193, Western blotting. All lanes: Anti FGFR4 (PB9193) at 0.5ug/ml. WB: Recombinant Human FGFR4 Protein 0.5ng. Predicted bind size: 39KD. Observed bind size: 39KD
图像 2 :
Anti- FGFR4 antibody, PB9193, Western blotting. All lanes: Anti FGFR4 (PB9193) at 0.5ug/ml. Lane 1: HELA Whole Cell Lysate at 40ug. Lane 2: PANC Whole Cell Lysate at 40ug. Lane 3: SGC Whole Cell Lysate at 40ug. Lane 4: COLO320 Whole Cell Lysate at 40ug. Lane 5: SW620 Whole Cell Lysate at 40ug. Predicted bind size: 88KD. Observed bind size: 100KD
图像 3 :
Anti- FGFR4 antibody, PB9193, IHC(P). IHC(P): Human Lung Cancer Tissue
产品信息
SKU号 :
PB9193
产品名称 :
Anti-FGFR4 Antibody Picoband™
价格 :
315美元
规格 :
100μg/vial
克隆性 :
多克隆
宿主 :
兔
反应物种 :
人类
应用(s) :
免疫组化, 免疫印迹
应用细节 :
Immunohistochemistry (Paraffin-embedded Section), 0.5-1µg/ml, Human, By Heat. Western blot, 0.1-0.5µg/ml, Human.
应用注释 :
WB: The detection limit for FGFR4 is approximately 0.25ng/lane under reducing conditions.
Tested Species: In-house tested species with positive results.
By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections.
Other applications have not been tested.
Optimal dilutions should be determined by end users.
描述 :
Boster Bio Anti-FGFR4 Antibody Picoband™ catalog # PB9193. Tested in IHC, WB applications. This antibody reacts with Human.
浓度 :
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
基因名称 :
FGFR4
UniProt数据库编号 :
P22455
免疫原 :
E.coli-derived human FGFR4 recombinant protein (Position: L22-H206). Human FGFR4 shares 86% and 84% amino acid (aa) sequences identity with mouse and rat FGFR4, respectively.
形式 :
冻干
Contents :
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
纯化 :
免疫原亲和纯化
交叉活性 :
No cross reactivity with other proteins
储存 :
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
重组 :
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
基因全称 :
成纤维细胞生长因子受体4
同义词 :
成纤维细胞生长因子受体4;FGFR-4;2.7。 10.1;CD334;FGFR4;JTK2, TKF;
蛋白名 :
成纤维细胞生长因子受体4
分子量 :
87954分子量
Protein Function :
Tyrosine-protein kinase that acts as cell-surface receptor for fibroblast growth factors and plays a role in the regulation of cell proliferation, differentiation and migration, and in regulation of lipid metabolism, bile acid biosynthesis, glucose uptake, vitamin D metabolism and phosphate homeostasis. Required for normal down-regulation of the expression of CYP7A1, the rate-limiting enzyme in bile acid synthesis, in response to FGF19. Phosphorylates PLCG1 and FRS2. Ligand binding leads to the activation of several signaling cascades. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. Phosphorylation of FRS2 triggers recruitment of GRB2, GAB1, PIK3R1 and SOS1, and mediates activation of RAS, MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling pathway, as well as of the AKT1 signaling pathway. Promotes SRC-dependent phosphorylation of the matrix protease MMP14 and its lysosomal degradation. FGFR4 signaling is down-regulated by receptor internalization and degradation; MMP14 promotes internalization and degradation of FGFR4. Mutations that lead to constitutive kinase activation or impair normal FGFR4 inactivation lead to aberrant signaling.
Subcellular Localization :
Cell membrane; Single-pass type I membrane protein. Endosome. Endoplasmic reticulum. Internalized from the cell membrane to recycling endosomes, and from there back to the cell membrane.
组织特异性 :
Expressed in gastrointestinal epithelial cells, pancreas, and gastric and pancreatic cancer cell lines.
Recommended Detection Systems :
Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
Sequence Similarities :
Belongs to the protein kinase superfamily. Tyr protein kinase family. Fibroblast growth factor receptor subfamily.
背景 :
FGFR4 (Fibroblast growth factor receptor 4), also known as CD334, is a protein that in humans is encoded by the FGFR4 gene. It is mapped to 5q35.2. The protein encoded by this gene is a member of the fibroblast growth factor receptor family, where amino acid sequence is highly conserved between members and throughout evolution. The extracellular portion of the protein interacts with fibroblast growth factors, setting in motion a cascade of downstream signals, ultimately influencing mitogenesis and differentiation. It is overexpressed in gynecological tumor samples, suggesting a role in breast and ovarian tumorigenesis.
研究种类 :
Angiogenesis, Cancer, Cardiovascular, Cell Biology, Cell Type Marker, Cell Type Markers, Growth Factors, Growth Factors/Hormones, Neural Stem Cells, Neuroscience, Signal Transduction, Stem Cells, Surface Molecules, Tags & Cell Markers
更多信息或购买 :
公司信息
武汉博士德生物工程有限公司
3942 B Valley Ave
Pleasanton, CA 94566
Pleasanton, CA 94566
boster@bosterbio.com
http://www.boster.com.cn925.485.4527
公司总部: 美国
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