产品简要
公司名称 :
武汉博士德生物工程有限公司
产品类型 :
抗体
产品名称 :
Anti-GCLC Antibody Picoband™
目录 :
PB9201
规格 :
100μg/vial
价格 :
315美元
克隆性 :
多克隆
宿主 :
domestic rabbit
共轭标签 :
未共轭
反应物种 :
人类, 大鼠
应用 :
免疫印迹, 免疫组化
图像
图像 1 :
武汉博士德生物工程有限公司 PB9201 图像 1
Anti- GCLC antibody, PB9201, Western blotting. All lanes: Anti GCLC (PB9201) at 0.5ug/ml. WB: Recombinant Human GCLC Protein 0.5ng. Predicted bind size: 40KD. Observed bind size: 40KD
图像 2 :
武汉博士德生物工程有限公司 PB9201 图像 2
Anti- GCLC antibody, PB9201, Western blotting. All lanes: Anti GCLC (PB9201) at 0.5ug/ml. Lane 1: Rat Brain Tissue Lysate at 50ug. Lane 2: Rat Cardiac Muscle Tissue Lysate at 50ug. Lane 3: HELA Cell Lysate at 40ug. Lane 4: PC-12 Whole Cell Lysate at 40ug. Lane 5: NRK Whole Cell Lysate at 40ug. Lane 6: HEPA Whole Cell Lysate at 40ug. Lane 7: A549 Whole Cell Lysate at 40ug. Predicted bind size: 72KD. Observed bind size: 72KD
图像 3 :
武汉博士德生物工程有限公司 PB9201 图像 3
Anti- GCLC antibody, PB9201, IHC(P). IHC(P): Human Lung Cancer Tissue
产品信息
SKU号 :
PB9201
产品名称 :
Anti-GCLC Antibody Picoband™
价格 :
315美元
规格 :
100μg/vial
克隆性 :
多克隆
宿主 :
反应物种 :
人类, 大鼠
应用(s) :
免疫组化, 免疫印迹
应用细节 :
Immunohistochemistry (Paraffin-embedded Section), 0.5-1µg/ml, Human, By Heat. Western blot, 0.1-0.5µg/ml, Human, Rat.
应用注释 :
WB: The detection limit for GCLC is approximately 0.2ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.
描述 :
Boster Bio Anti-GCLC Antibody Picoband™ catalog # PB9201. Tested in IHC, WB applications. This antibody reacts with Human, Rat.
浓度 :
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
基因名称 :
GCLC
UniProt数据库编号 :
P48506
免疫原 :
E.coli-derived human GCLC recombinant protein (Position: E437-N637). Human GCLC shares 94% amino acid (aa) sequence identity with both mouse and rat GCLC.
形式 :
冻干
Contents :
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
纯化 :
免疫原亲和纯化
交叉活性 :
No cross reactivity with other proteins
储存 :
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
重组 :
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
基因全称 :
Glutamate--cysteine ligase catalytic subunit
同义词 :
Glutamate--cysteine ligase catalytic subunit; 6.3.2.2; GCS heavy chain; Gamma-ECS; Gamma-glutamylcysteine synthetase; GCLC; GLCL, GLCLC;
蛋白名 :
Glutamate--cysteine ligase catalytic subunit
分子量 :
72766分子量
Recommended Detection Systems :
Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
Sequence Similarities :
Belongs to the glutamate--cysteine ligase type 3 family.
背景 :
GCLC, also named Glutamate--cysteine ligase catalytic subunit, is an enzyme that in humans is encoded by the GCLC gene. Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. The gene encoding the catalytic subunit encodes a protein of 367 amino acids with a calculated molecular weight of 72.773 kDa and maps to chromosome 6p12.1. Deficiency of gamma-glutamylcysteine synthetase in human is associated with enzymopathic hemolytic anemia.
研究种类 :
Cancer, Cell Biology, Metabolism, Mitochondrial, Mitochondrial Markers, Mitochondrial Metabolism, Oxidative Stress, Pathways And Processes, Redox Metabolism, Signal Transduction
公司信息
武汉博士德生物工程有限公司
3942 B Valley Ave
Pleasanton, CA 94566
boster@bosterbio.com
http://www.boster.com.cn
925.485.4527
公司总部: 美国
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