抗体

Anti-MUM1/IRF4 Antibody Picoband™

PB9222

100μg/vial

315美元

多克隆

domestic rabbit

未共轭

免疫印迹, 免疫组化

Anti-MUM1/IRF4 Antibody Picoband™

315美元

100μg/vial

多克隆

兔

人类

免疫组化, 免疫印迹

Immunohistochemistry (Paraffin-embedded Section), 0.5-1µg/ml, Human, By Heat. Western blot, 0.1-0.5µg/ml, Human.

WB: The detection limit for MUM1 is approximately 0.2ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Boster Bio Anti-MUM1/IRF4 Antibody Picoband™ catalog # PB9222. Tested in IHC, WB applications. This antibody reacts with Human.

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

IRF4

Q15306

E.coli-derived human MUM1 recombinant protein (Position: E272-E451). Human MUM1 shares 92% amino acid (aa) sequence identity with mouse MUM1.

冻干

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

免疫原亲和纯化

No cross reactivity with other proteins

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

干扰素调节因子4

Interferon regulatory factor 4; IRF-4; Lymphocyte-specific interferon regulatory factor; LSIRF; Multiple myeloma oncogene 1; NF-EM5; IRF4; MUM1;

干扰素调节因子4

51772分子量

Transcriptional activator. Binds to the interferon- stimulated response element (ISRE) of the MHC class I promoter. Binds the immunoglobulin lambda light chain enhancer, together with PU.1. Probably plays a role in ISRE-targeted signal transduction mechanisms specific to lymphoid cells. Involved in CD8 (+) dendritic cell differentiation by forming a complex with the BATF-JUNB heterodimer in immune cells, leading to recognition of AICE sequence (5'-TGAnTCA/GAAA-3'), an immune-specific regulatory element, followed by cooperative binding of BATF and IRF4 and activation of genes (By similarity).

Nucleus.

Lymphoid cells.

Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).

Belongs to the IRF family.

Interferon regulatory factor 4 (IRF4), also known as MUM1, is a protein that in humans is encoded by the IRF4 gene. It is located on 6p25.3. IRF4 is a transcription factor, and it is essential for the development of T helper-2 (Th2) cells, IL17 -producing Th17 cells, and IL9 -producing Th9 cells. In melanocytic cells, the IRF4 gene may be regulated by MITF. IRF4 is a transcription factor that has been implicated in acute leukemia. This gene is strongly associated with pigmentation, sensitivity of skin to sun exposure, freckles, blue eyes, and brown hair color. What's more, IRF4 inhibition is toxic to myeloma cell lines, regardless of transforming oncogenic mechanism.

Cancer, Cancer Susceptibility, Cell Type Markers, Epigenetics and Nuclear Signaling, Oncoproteins, Oncoproteins/Suppressors, Proto-Oncogenes, Tags & Cell Markers, Transcription, Transcription Factors, Tumor Associated