产品简要
公司名称 :
武汉博士德生物工程有限公司
产品类型 :
抗体
产品名称 :
Anti-Transferrin Receptor/TFRC Antibody Picoband™
目录 :
PB9233
规格 :
100μg/vial
价格 :
315美元
克隆性 :
多克隆
宿主 :
domestic rabbit
共轭标签 :
未共轭
反应物种 :
人类
应用 :
免疫印迹, 免疫细胞化学, 流式细胞仪, 免疫组化-石蜡切片, 免疫组化-冰冻切片
图像
图像 1 :
武汉博士德生物工程有限公司 PB9233 图像 1
Anti- TFRC antibody, PB9233, Western blotting. All lanes: Anti TFRC (PB9233) at 0.5ug/ml. WB: Recombinant Human TRFC Protein 0.5ng. Predicted bind size: 45KD. Observed bind size: 45KD
图像 2 :
武汉博士德生物工程有限公司 PB9233 图像 2
Anti- TFRC antibody, PB9233, Western blotting. All lanes: Anti TRFC (PB9233) at 0.5ug/ml. Lane 1: HELA Whole Cell Lysate at 40ug. Lane 2: JURAKT Whole Cell Lysate at 40ug. Lane 3: RAJI Whole Cell Lysate at 40ug. Lane 4: HL-60 Whole Cell Lysate at 40ug. Lane 5: K562 Whole Cell Lysate at 40ug. Lane 6: HEPG2 Whole Cell Lysate at 40ug. Lane 7: Human Placenta Tissue Lysate at 50ug. Lane 8: CEM Whole Cell Lysate at 40ug. Predicted bind size: 86KD. Observed bind size: 98KD
图像 3 :
武汉博士德生物工程有限公司 PB9233 图像 3
Anti- TFRC antibody, PB9233,IHC(P). IHC(P): Human Placenta Tissue
产品信息
SKU号 :
PB9233
产品名称 :
Anti-Transferrin Receptor/TFRC Antibody Picoband™
价格 :
315美元
规格 :
100μg/vial
克隆性 :
多克隆
宿主 :
反应物种 :
人类
应用(s) :
流式细胞仪, IF, 免疫组化-P, 免疫组化-F, 免疫细胞化学, 免疫印迹
应用细节 :
Western blot, 0.1-0.5µg/ml, Human. Immunohistochemistry (Paraffin-embedded Section), 0.5-1µg/ml, Human, By Heat. Immunohistochemistry (Frozen Section), 0.5-1µg/ml, Human. Immunocytochemistry/Immunofluorescence, 2µg/ml, Human. Flow Cytometry, 1-3µg/1x10^6 cells, Human.
应用注释 :
WB: The detection limit for TFRC is approximately 0.25ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.
描述 :
Boster Bio Anti-Transferrin Receptor/TFRC Antibody Picoband™ catalog # PB9233. Tested in Flow Cytometry, IF, IHC-P, IHC-F, ICC, WB applications. This antibody reacts with Human.
浓度 :
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
基因名称 :
转铁蛋白受体
UniProt数据库编号 :
P02786
免疫原 :
E.coli-derived human TFRC recombinant protein (Position: M1-N198). Human TFRC shares 72% and 70% amino acid (aa) sequences identity with mouse and rat TFRC, respectively.
形式 :
冻干
Contents :
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
纯化 :
免疫原亲和纯化
交叉活性 :
No cross reactivity with other proteins
储存 :
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
重组 :
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
基因全称 :
转铁蛋白受体蛋白1
同义词 :
Transferrin receptor protein 1; TR; TfR; TfR1; Trfr; T9; p90; CD71; Transferrin receptor protein 1, serum form; sTfR; TFRC;
蛋白名 :
转铁蛋白受体蛋白1
分子量 :
84871分子量
Protein Function :
Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system (By similarity). A second ligand, the heditary hemochromatosis protein HFE, competes for binding with transferrin for an overlapping C-terminal binding site.
Subcellular Localization :
Cell membrane ; Single-pass type II membrane protein. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
Recommended Detection Systems :
Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P), IHC(F) and ICC.
Sequence Similarities :
Belongs to the peptidase M28 family. M28B subfamily.
背景 :
Transferrin receptor protein 1 (TfR1), also known as Cluster of Differentiation 71 (CD71), is a protein that in humans is encoded by the TFRC gene. It is mapped to 3q29. TFRC is a transmembrane glycoprotein composed of two disulfide-linked monomers joined by two disulfide bonds. Expression of human TFR1 in hamster cell lines markedly enhanced the infection of viruses pseudotyped with the glycoprotein of Machupo, Guanarito, and Junin viruses. TFR1 is a cellular receptor for New World hemorrhagic fever arenaviruses. It is required for iron delivery from transferrin to cells.
研究种类 :
Serum Proteins, Stem Cells, Surface Molecules, T Lymphocytic Lineage
公司信息
武汉博士德生物工程有限公司
3942 B Valley Ave
Pleasanton, CA 94566
boster@bosterbio.com
http://www.boster.com.cn
925.485.4527
公司总部: 美国
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