产品简要
公司名称 :
武汉博士德生物工程有限公司
产品类型 :
抗体
产品名称 :
Anti-Transferrin Receptor/TFRC Picoband Antibody
目录 :
PB9233
规格 :
100 ug/vial
价格 :
280美元
克隆性 :
多克隆
宿主 :
共轭标签 :
nonconjugated
反应物种 :
人类
应用 :
免疫印迹, 免疫细胞化学, 流式细胞仪, 免疫组化-石蜡切片, 免疫组化-冰冻切片
图像
图像 1 :
武汉博士德生物工程有限公司 PB9233 图像 1
Anti- TFRC antibody, PB9233, Western blotting. All lanes: Anti TFRC (PB9233) at 0.5ug/ml. WB: Recombinant Human TRFC Protein 0.5ng. Predicted bind size: 45KD. Observed bind size: 45KD
图像 2 :
武汉博士德生物工程有限公司 PB9233 图像 2
Anti- TFRC antibody, PB9233, Western blotting. All lanes: Anti TRFC (PB9233) at 0.5ug/ml. Lane 1: HELA Whole Cell Lysate at 40ug. Lane 2: JURAKT Whole Cell Lysate at 40ug. Lane 3: RAJI Whole Cell Lysate at 40ug. Lane 4: HL-60 Whole Cell Lysate at 40ug. Lane 5: K562 Whole Cell Lysate at 40ug. Lane 6: HEPG2 Whole Cell Lysate at 40ug. Lane 7: Human Placenta Tissue Lysate at 50ug. Lane 8: CEM Whole Cell Lysate at 40ug. Predicted bind size: 86KD. Observed bind size: 98KD
图像 3 :
武汉博士德生物工程有限公司 PB9233 图像 3
Anti- TFRC antibody, PB9233,IHC(P). IHC(P): Human Placenta Tissue
产品信息
SKU号 :
PB9233
状态 :
Enabled
名称 :
Anti-Transferrin Receptor/TFRC Picoband Antibody
目录名称 :
一抗, 多克隆抗体, 免疫组化免疫细胞化学IF抗体
基因名称 :
转铁蛋白受体
价格 :
280美元
price various sizes :
Unconjugated / $280 APC / $330 APC-Cy7 / $330 FITC / $330 PE / $330 PE-Cy5 / $330 PE-Cy7 / $330 30ug sample size / $99 100ug / $280 100ug+Free HRP Secondary BA1054 / $280 100ug+Free Biotin Secondary BA1003 / $280
克隆性 :
多克隆
浓度 :
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
共轭标签 :
No
contents :
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
描述 :
Polyclonal antibody for TRANSFERRIN RECEPTOR/TFRC detection. Host: Rabbit.Size: 100ug/vial. Tested applications: WB, IHC-P, IHC-F, ICC/IF, FCM. Reactive species: Human. TRANSFERRIN RECEPTOR/TFRC information: Molecular Weight: 84871 MW; Subcellular Localization: Cell membrane ; Single-pass type II membrane protein . Melanosome . Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
short description :
Rabbit IgG polyclonal antibody for Transferrin receptor protein 1(TFRC) detection. Tested with WB, IHC-P, IHC-F, ICC/IF, FCM in Human.
规格 :
100 ug/vial
sample size available :
30ug for $99, contact us for details
UniProt数据库编号 :
P02786
宿主 :
免疫原 :
E.coli-derived human TFRC recombinant protein (Position: M1-N198). Human TFRC shares 72% and 70% amino acid (aa) sequences identity with mouse and rat TFRC, respectively.
形式 :
冻干
纯化 :
免疫原亲和纯化
储存 :
At -20 C for one year. After reconstitution, at 4 C for one month. It can also be aliquotted and stored frozen at -20 C for a longer time. Avoid repeated freezing and thawing.
交叉活性 :
No cross reactivity with other proteins
重组 :
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
应用细节 :
Western blot, 0.1-0.5ug/ml, Human. Immunohistochemistry(Paraffin-embedded Section), 0.5-1ug/ml, Human, By Heat. Immunohistochemistry(Frozen Section), 0.5-1ug/ml, Human. Immunocytochemistry/Immunofluorescence, 2ug/ml, Human. Flow Cytometry, 1-3ug/1x10 6 cells, Human.
应用 :
流式细胞仪, IF, 免疫组化-P, 免疫组化-F, 免疫细胞化学, 免疫印迹
反应物种 :
人类
图像标记 :
Anti- TFRC antibody, PB9233, Western blotting. All lanes: Anti TFRC (PB9233) at 0.5ug/ml. WB: Recombinant Human TRFC Protein 0.5ng. Predicted bind size: 45KD. Observed bind size: 45KD Anti- TFRC antibody, PB9233, Western blotting. All lanes: Anti TRFC (PB9233) at 0.5ug/ml. Lane 1: HELA Whole Cell Lysate at 40ug. Lane 2: JURAKT Whole Cell Lysate at 40ug. Lane 3: RAJI Whole Cell Lysate at 40ug. Lane 4: HL-60 Whole Cell Lysate at 40ug. Lane 5: K562 Whole Cell Lysate at 40ug. Lane 6: HEPG2 Whole Cell Lysate at 40ug. Lane 7: Human Placenta Tissue Lysate at 50ug. Lane 8: CEM Whole Cell Lysate at 40ug. Predicted bind size: 86KD. Observed bind size: 98KD Anti- TFRC antibody, PB9233,IHC(P). IHC(P): Human Placenta Tissue Figure 4. IHC analysis of TFRC using anti-TFRC antibody (PB9233). TFRC was detected in frozen section of human placenta tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-TFRC Antibody (PB9233) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. Figure 5. IF analysis of TFRC using anti-TFRC antibody (PB9233) . TFRC was detected in immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/mL rabbit anti-TFRC Antibody (PB9233) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. Figure 6. Flow Cytometry analysis of SiHa cells using anti-TFRC antibody (PB9233). Overlay histogram showing SiHa cells stained with PB9233 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TFRC Antibody (PB9233, 1ug/1x10 6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10ug/1x10 6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x10 6 ) used under the same conditions. Unlabelled sample (Red line) was also used as a control. Figure 7. Flow Cytometry analysis of U87 cells using anti-TFRC antibody (PB9233). Overlay histogram showing U87 cells stained with PB9233 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TFRC Antibody (PB9233, 1ug/1x10 6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10ug/1x10 6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x10 6 ) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
背景 :
Transferrin receptor protein 1 (TfR1), also known as Cluster of Differentiation 71 (CD71), is a protein that in humans is encoded by the TFRC gene. It is mapped to 3q29. TFRC is a transmembrane glycoprotein composed of two disulfide-linked monomers joined by two disulfide bonds. Expression of human TFR1 in hamster cell lines markedly enhanced the infection of viruses pseudotyped with the glycoprotein of Machupo, Guanarito, and Junin viruses. TFR1 is a cellular receptor for New World hemorrhagic fever arenaviruses. It is required for iron delivery from transferrin to cells.
competitor equivalent skus :
sc 19675 sc 32272 sc 393719 sc 51829 sc 59111 sc 65877 sc 65882 sc 7087 sc 7088 sc 7327 sc 80659 sc 80660 sc 80661 sc 80662 sc 9099
研究种类 :
Serum Proteins, Stem Cells, Surface Molecules, T Lymphocytic Lineage
同义词 :
Transferrin receptor protein 1;TR;TfR;TfR1;Trfr;T9;p90;CD71;Transferrin receptor protein 1, serum form;sTfR;TFRC;
基因全称 :
转铁蛋白受体蛋白1
分子量 :
84871分子量
protein function :
Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system (By similarity). A second ligand, the heditary hemochromatosis protein HFE, competes for binding with transferrin for an overlapping C-terminal binding site.
subcellular localization :
Cell membrane ; Single-pass type II membrane protein . Melanosome . Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
蛋白名 :
转铁蛋白受体蛋白1
recommended detection systems :
Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P), IHC(F) and ICC.
sequence similarities :
Belongs to the peptidase M28 family. M28B subfamily.
更新 :
5/5/19 7:51
公司信息
武汉博士德生物工程有限公司
3942 B Valley Ave
Pleasanton, CA 94566
boster@bosterbio.com
http://www.boster.com.cn
925.485.4527
公司总部: 美国
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