产品简要
公司名称 :
武汉博士德生物工程有限公司
产品类型 :
抗体
产品名称 :
Anti-Smad Interacting Protein 1/ZEB2 Picoband Antibody
目录 :
PB9236
规格 :
100 ug/vial
价格 :
280美元
克隆性 :
多克隆
宿主 :
共轭标签 :
nonconjugated
反应物种 :
人类
应用 :
免疫印迹, 免疫细胞化学, 流式细胞仪, 免疫组化-石蜡切片, 免疫组化-冰冻切片
图像
图像 1 :
武汉博士德生物工程有限公司 PB9236 图像 1
Anti- ZEB2antibody, PB9236, Western blotting. All lanes: Anti ZEB2 (PB9236) at 0.5ug/ml. WB: Recombinant Human ZEB2 Protein 0.5ng. Predicted bind size: 45KD. Observed bind size: 45KD
图像 2 :
武汉博士德生物工程有限公司 PB9236 图像 2
Anti- ZEB2antibody, PB9236, Western blotting. All lanes: Anti ZEB2 (PB9236) at 0.5ug/ml. WB: SMMC Whole Cell Lysate at 40ug. Predicted bind size: 136KD. Observed bind size: 136KD
图像 3 :
武汉博士德生物工程有限公司 PB9236 图像 3
Figure 3. Flow Cytometry analysis of U937 cells using anti-ZEB2 antibody (PB9236). Overlay histogram showing U937 cells stained with PB9236 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ZEB2 Antibody (PB9236,1ug/1x10 6 cells) for 30 min at 20°C. DyLight 488 conjugated goat anti-rabbit IgG (BA1127, 5-10ug/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x10 6 ) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
产品信息
SKU号 :
PB9236
状态 :
Enabled
名称 :
Anti-Smad Interacting Protein 1/ZEB2 Picoband Antibody
目录名称 :
一抗, 多克隆抗体, 免疫组化免疫细胞化学IF抗体
基因名称 :
ZEB2
价格 :
280美元
price various sizes :
30ug sample size / $99 100ug / $280 100ug+Free HRP Secondary BA1054 / $280 100ug+Free Biotin Secondary BA1003 / $280
克隆性 :
多克隆
浓度 :
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
共轭标签 :
No
contents :
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
描述 :
Polyclonal antibody for SIP1/ZEB2 detection. Host: Rabbit.Size: 100ug/vial. Tested applications: WB, IHC-P, IHC-F, ICC, FCM. Reactive species: Human, Mouse, Rat. SIP1/ZEB2 information: Molecular Weight: 136447 MW; Subcellular Localization: Nucleus .
short description :
Rabbit IgG polyclonal antibody for Zinc finger E-box-binding homeobox 2(ZEB2) detection. Tested with WB, IHC-P, IHC-F, ICC, FCM in Human; Mouse; Rat.
规格 :
100 ug/vial
sample size available :
30ug for $99, contact us for details
UniProt数据库编号 :
O60315
宿主 :
免疫原 :
E.coli-derived human ZEB2 recombinant protein (Position: M1-P200). Human ZEB2 shares 92% amino acid (aa) sequence identity with mouse ZEB2.
形式 :
冻干
纯化 :
免疫原亲和纯化
储存 :
At -20 C for one year. After reconstitution, at 4 C for one month. It can also be aliquotted and stored frozen at -20 C for a longer time.Avoid repeated freezing and thawing.
交叉活性 :
No cross reactivity with other proteins
重组 :
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
应用细节 :
Western blot, 0.1-0.5ug/ml, Human. Immunohistochemistry(Paraffin-embedded Section), 0.5-1ug/ml, Human, Mouse, Rat, By Heat. Immunohistochemistry(Frozen Section), 0.5-1ug/ml, Human. Immunocytochemistry, 0.5-1ug/ml, Human. Flow Cytometry, 1-3ug/1x10 6 cells, Human
应用 :
流式细胞仪, 免疫组化-P, 免疫组化-F, 免疫细胞化学, 免疫印迹
反应物种 :
人类
图像标记 :
Anti- ZEB2antibody, PB9236, Western blotting. All lanes: Anti ZEB2 (PB9236) at 0.5ug/ml. WB: Recombinant Human ZEB2 Protein 0.5ng. Predicted bind size: 45KD. Observed bind size: 45KD Anti- ZEB2antibody, PB9236, Western blotting. All lanes: Anti ZEB2 (PB9236) at 0.5ug/ml. WB: SMMC Whole Cell Lysate at 40ug. Predicted bind size: 136KD. Observed bind size: 136KD Figure 3. Flow Cytometry analysis of U937 cells using anti-ZEB2 antibody (PB9236). Overlay histogram showing U937 cells stained with PB9236 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ZEB2 Antibody (PB9236,1ug/1x10 6 cells) for 30 min at 20°C. DyLight 488 conjugated goat anti-rabbit IgG (BA1127, 5-10ug/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x10 6 ) used under the same conditions. Unlabelled sample (Red line) was also used as a control. Figure 4. Flow Cytometry analysis of THP-1 cells using anti-ZEB2 antibody (PB9236). Overlay histogram showing THP-1 cells stained with PB9236 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ZEB2 Antibody (PB9236,1ug/1x10 6 cells) for 30 min at 20°C. DyLight 488 conjugated goat anti-rabbit IgG (BA1127, 5-10ug/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x10 6 ) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
背景 :
ZEB2(Zinc finger E-box-binding homeobox2), also known as SIP1 or ZINC FINGER HOMEOBOX 1B(ZFHX1B), is a protein that in humans is encoded by the ZEB2 gene. The ZEB2 gene is a member of the ZEB1/Drosophila Zfh1 family of 2-handed zinc finger/homeodomain proteins and functions as a DNA-binding transcriptional repressor that interacts with activated SMADs, the transducers of TGF-beta signaling, and interacts with the nucleosome remodeling and histone deacetylation(NURD) complex. By radiation hybrid analysis, this gene is mapped to 2q22. It has been found that synthesis of ZEB2 was upregulated following SNAI1 expression in human cell lines, and the expression of SNAI1 in epithelial cells can trigger an epithelial-mesenchyme transition.
competitor equivalent skus :
sc 48789 sc 18392 sc 271984
研究种类 :
Cancer, Developmental Biology, Developmental Families, Domain Families, Epigenetics And Nuclear Signaling, Lineage Markers, Lineage Specification, Neurogenesis, Neurology Process, Neuroscience, Oncoproteins, Oncoproteins/Suppressors, Stem Cells, Transcription, Transcription Factors
同义词 :
Zinc finger E-box-binding homeobox 2;Smad-interacting protein 1;SMADIP1;Zinc finger homeobox protein 1b;ZEB2;KIAA0569, SIP1, ZFHX1B, ZFX1B;HRIHFB2411;
基因全称 :
锌指E拳击同源异形盒2
分子量 :
136447分子量
protein function :
Transcriptional inhibitor that binds to DNA sequence 5'- CACCT-3' in different promoters. Represses transcription of E- cadherin.
subcellular localization :
Nucleus .
蛋白名 :
锌指E拳击同源异形盒2
recommended detection systems :
Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, supported by SA1022 in IHC(P) and IHC(F).
sequence similarities :
Belongs to the delta-EF1/ZFH-1 C2H2-type zinc-finger family.
更新 :
6/5/19 9:02
公司信息
武汉博士德生物工程有限公司
3942 B Valley Ave
Pleasanton, CA 94566
boster@bosterbio.com
http://www.boster.com.cn
925.485.4527
公司总部: 美国
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