产品简要
公司名称 :
武汉博士德生物工程有限公司
产品类型 :
抗体
产品名称 :
Anti-Smad Interacting Protein 1/ZEB2 Antibody Picoband™
目录 :
PB9236
规格 :
100μg/vial
价格 :
315美元
克隆性 :
多克隆
宿主 :
domestic rabbit
共轭标签 :
未共轭
反应物种 :
人类
应用 :
免疫印迹, 免疫细胞化学, 流式细胞仪, 免疫组化-石蜡切片, 免疫组化-冰冻切片
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图像
图像 1 :
Anti- ZEB2antibody, PB9236, Western blotting. All lanes: Anti ZEB2 (PB9236) at 0.5ug/ml. WB: Recombinant Human ZEB2 Protein 0.5ng. Predicted bind size: 45KD. Observed bind size: 45KD
图像 2 :
Anti- ZEB2antibody, PB9236, Western blotting. All lanes: Anti ZEB2 (PB9236) at 0.5ug/ml. WB: SMMC Whole Cell Lysate at 40ug. Predicted bind size: 136KD. Observed bind size: 136KD
图像 3 :
Figure 3. Flow Cytometry analysis of U937 cells using anti-ZEB2 antibody (PB9236). Overlay histogram showing U937 cells stained with PB9236 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ZEB2 Antibody (PB9236,1ug/1x10 6 cells) for 30 min at 20°C. DyLight 488 conjugated goat anti-rabbit IgG (BA1127, 5-10ug/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x10 6 ) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
产品信息
SKU号 :
PB9236
产品名称 :
Anti-Smad Interacting Protein 1/ZEB2 Antibody Picoband™
价格 :
315美元
规格 :
100μg/vial
克隆性 :
多克隆
宿主 :
兔
反应物种 :
人类
应用(s) :
流式细胞仪, 免疫组化-P, 免疫组化-F, 免疫细胞化学, 免疫印迹
应用细节 :
Western blot, 0.1-0.5µg/ml, Human.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1µg/ml, Human, Mouse, Rat, By Heat.
Immunohistochemistry (Frozen Section), 0.5-1µg/ml, Human.
Immunocytochemistry, 0.5-1µg/ml, Human.
Flow Cytometry, 1-3µg/1x10^6 cells, Human
应用注释 :
WB: The detection limit for ZEB2 is approximately 0.2ng/lane under reducing conditions.
Tested Species: In-house tested species with positive results.
By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections.
Other applications have not been tested.
Optimal dilutions should be determined by end users.
描述 :
Boster Bio Anti-Smad Interacting Protein 1/ZEB2 Antibody Picoband™ catalog # PB9236. Tested in Flow Cytometry, IHC-P, IHC-F, ICC, WB applications. This antibody reacts with Human.
浓度 :
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
基因名称 :
ZEB2
UniProt数据库编号 :
O60315
免疫原 :
E.coli-derived human ZEB2 recombinant protein (Position: M1-P200). Human ZEB2 shares 92% amino acid (aa) sequence identity with mouse ZEB2.
形式 :
冻干
Contents :
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
纯化 :
免疫原亲和纯化
交叉活性 :
No cross reactivity with other proteins
储存 :
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
重组 :
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
基因全称 :
锌指E拳击同源异形盒2
同义词 :
Zinc finger E-box-binding homeobox 2; Smad-interacting protein 1; SMADIP1; Zinc finger homeobox protein 1b; ZEB2; KIAA0569, SIP1, ZFHX1B, ZFX1B; HRIHFB2411;
蛋白名 :
锌指E拳击同源异形盒2
分子量 :
136447分子量
Protein Function :
Transcriptional inhibitor that binds to DNA sequence 5'- CACCT-3' in different promoters. Represses transcription of E- cadherin.
Subcellular Localization :
Nucleus.
Recommended Detection Systems :
Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, supported by SA1022 in IHC(P) and IHC(F).
Sequence Similarities :
Belongs to the delta-EF1/ZFH-1 C2H2-type zinc-finger family.
背景 :
ZEB2 (Zinc finger E-box-binding homeobox2), also known as SIP1 or ZINC FINGER HOMEOBOX 1B (ZFHX1B), is a protein that in humans is encoded by the ZEB2 gene. The ZEB2 gene is a member of the ZEB1/Drosophila Zfh1 family of 2-handed zinc finger/homeodomain proteins and functions as a DNA-binding transcriptional repressor that interacts with activated SMADs, the transducers of TGF-beta signaling, and interacts with the nucleosome remodeling and histone deacetylation (NURD) complex. By radiation hybrid analysis, this gene is mapped to 2q22. It has been found that synthesis of ZEB2 was upregulated following SNAI1 expression in human cell lines, and the expression of SNAI1 in epithelial cells can trigger an epithelial-mesenchyme transition.
研究种类 :
Cancer, Developmental Biology, Developmental Families, Domain Families, Epigenetics and Nuclear Signaling, Lineage Markers, Lineage Specification, Neurogenesis, Neurology Process, Neuroscience, Oncoproteins, Oncoproteins/Suppressors, Stem Cells, Transcription, Transcription Factors
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公司信息
武汉博士德生物工程有限公司
3942 B Valley Ave
Pleasanton, CA 94566
Pleasanton, CA 94566
boster@bosterbio.com
http://www.boster.com.cn925.485.4527
公司总部: 美国
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