产品简要
公司名称 :
武汉博士德生物工程有限公司
产品类型 :
抗体
产品名称 :
Anti-Hsp27/HSPB1 Picoband Antibody
目录 :
PB9237
规格 :
100 ug/vial
价格 :
280美元
克隆性 :
多克隆
宿主 :
共轭标签 :
nonconjugated
反应物种 :
人类
应用 :
免疫印迹, 免疫细胞化学, 流式细胞仪, 免疫组化-石蜡切片, 免疫组化-冰冻切片
图像
图像 1 :
武汉博士德生物工程有限公司 PB9237 图像 1
Anti- HSP27 antibody, PB9237, Western blotting. All lanes: Anti HSP27 (PB9237) at 0.5ug/ml. WB: Recombinant Human HSP27 Protein 0.5ng. Predicted bind size: 19KD. Observed bind size: 19KD
图像 2 :
武汉博士德生物工程有限公司 PB9237 图像 2
Anti- HSP27 antibody, PB9237, Western blotting. All lanes: Anti HSP27 (PB9237) at 0.5ug/ml. Lane 1: NRK Whole Cell Lysate at 40ug. Lane 2: HELA Whole Cell Lysate at 40ug. Lane 3: A549 Whole Cell Lysate at 40ug. Lane 4: COLO320 Whole Cell Lysate at 40ug. Lane 5: HEPG2 Whole Cell Lysate at 40ug. Predicted bind size: 23KD. Observed bind size: 23KD
图像 3 :
武汉博士德生物工程有限公司 PB9237 图像 3
Anti- HSP27 antibody, PB9237, IHC(P). IHC(P): Human Intestinal Cancer Tissue
产品信息
SKU号 :
PB9237
状态 :
Enabled
名称 :
Anti-Hsp27/HSPB1 Picoband Antibody
目录名称 :
一抗, 多克隆抗体, 免疫组化免疫细胞化学IF抗体
基因名称 :
HSPB1
价格 :
280美元
price various sizes :
Unconjugated / $280 APC / $330 APC-Cy7 / $330 FITC / $330 PE / $330 PE-Cy5 / $330 PE-Cy7 / $330 30ug sample size / $99 100ug / $280 100ug+Free HRP Secondary BA1054 / $280 100ug+Free Biotin Secondary BA1003 / $280
克隆性 :
多克隆
浓度 :
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
共轭标签 :
No
contents :
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
描述 :
Polyclonal antibody for HSP27/HSPB1 detection. Host: Rabbit.Size: 100ug/vial. Tested applications: WB, IHC-P, IHC-F, ICC/IF, FCM. Reactive species: Human. HSP27/HSPB1 information: Molecular Weight: 22783 MW; Subcellular Localization: Cytoplasm. Nucleus. Cytoplasm, cytoskeleton, spindle. Cytoplasmic in interphase cells. Colocalizes with mitotic spindles in mitotic cells. Translocates to the nucleus during heat shock and resides in sub-nuclear structures known as SC35 speckles or nuclear splicing speckles; Tissue Specificity: Detected in all tissues tested: skeletal muscle, heart, aorta, large intestine, small intestine, stomach, esophagus, bladder, adrenal gland, thyroid, pancreas, testis, adipose tissue, kidney, liver, spleen, cerebral cortex, blood serum and cerebrospinal fluid. Highest levels are found in the heart and in tissues composed of striated and smooth muscle.
short description :
Rabbit IgG polyclonal antibody for Heat shock protein beta-1(HSPB1) detection. Tested with WB, IHC-P, IHC-F, ICC/IF, FCM in Human.
规格 :
100 ug/vial
sample size available :
30ug for $99, contact us for details
UniProt数据库编号 :
P04792
宿主 :
免疫原 :
E.coli-derived human Hsp27 recombinant protein (Position: M1-K205). Human Hsp27 shares 83% amino acid (aa) sequence identity with mouse Hsp27.
形式 :
冻干
纯化 :
免疫原亲和纯化
储存 :
At -20 C for one year. After reconstitution, at 4 C for one month. It can also be aliquotted and stored frozen at -20 C for a longer time. Avoid repeated freezing and thawing.
交叉活性 :
No cross reactivity with other proteins
重组 :
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
应用细节 :
Western blot, 0.1-0.5ug/ml, Human. Immunohistochemistry(Paraffin-embedded Section), 0.5-1ug/ml, Human, By Heat. Immunohistochemistry(Frozen Section), 0.5-1ug/ml, Human, -. Immunocytochemistry/Immunofluorescence, 2ug/ml, Human, -. Flow Cytometry, 1-3ug/mlx10 6 cells, Human, -.
应用 :
流式细胞仪, IF, 免疫组化-P, 免疫组化-F, 免疫细胞化学, 免疫印迹
反应物种 :
人类
图像标记 :
Anti- HSP27 antibody, PB9237, Western blotting. All lanes: Anti HSP27 (PB9237) at 0.5ug/ml. WB: Recombinant Human HSP27 Protein 0.5ng. Predicted bind size: 19KD. Observed bind size: 19KD Anti- HSP27 antibody, PB9237, Western blotting. All lanes: Anti HSP27 (PB9237) at 0.5ug/ml. Lane 1: NRK Whole Cell Lysate at 40ug. Lane 2: HELA Whole Cell Lysate at 40ug. Lane 3: A549 Whole Cell Lysate at 40ug. Lane 4: COLO320 Whole Cell Lysate at 40ug. Lane 5: HEPG2 Whole Cell Lysate at 40ug. Predicted bind size: 23KD. Observed bind size: 23KD Anti- HSP27 antibody, PB9237, IHC(P). IHC(P): Human Intestinal Cancer Tissue Anti- HSP27 antibody, PB9237, IHC(P). IHC(P): Human Mammary Cancer Tissue Anti- HSP27 antibody, PB9237, IHC(F). IHC(F): Human Placenta Tissue Figure 6. IF analysis of HSP27 using anti-HSP27 antibody (PB9237) . HSP27 was detected in immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/mL rabbit anti-HSP27 Antibody (PB9237) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. Figure 7. Flow Cytometry analysis of A431 cells using anti-HSP27 antibody (PB9237). Overlay histogram showing A431 cells stained with PB9237 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti- HSP27 Antibody (PB9237,1ug/1x10 6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10ug/1x10 6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x10 6 ) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
背景 :
HSPB1(Heat shock 27kDa protein 1), also known as HSP27, is a protein that in humans is encoded by the HSPB1 gene. HSP27 gene is mapped to 7q11.23. The protein encoded by this gene is induced by environmental stress and developmental changes. The encoded protein is involved in stress resistance and actin organization and translocates from the cytoplasm to the nucleus upon stress induction. Defects in this gene are a cause of Charcot-Marie-Tooth disease type 2F (CMT2F) and distal hereditary motor neuropathy (dHMN).
competitor equivalent skus :
sc 22707 sc 7768 sc 34120 sc 28880 sc 34117 sc 7767 sc 34119
研究种类 :
Atherosclerosis, Cancer, Cardiovascular, Chaperones, Contractility, Heat Shock Proteins, Protein Trafficking, Signal Transduction, Tumor Biomarkers
同义词 :
Heat shock protein beta-1;HspB1;28 kDa heat shock protein;Estrogen-regulated 24 kDa protein;Heat shock 27 kDa protein;HSP 27;Stress-responsive protein 27;SRP27;HSPB1;HSP27, HSP28;
基因全称 :
热休克蛋白乙1
分子量 :
22783分子量
protein function :
Involved in stress resistance and actin organization.
subcellular localization :
Cytoplasm. Nucleus. Cytoplasm, cytoskeleton, spindle. Cytoplasmic in interphase cells. Colocalizes with mitotic spindles in mitotic cells. Translocates to the nucleus during heat shock and resides in sub-nuclear structures known as SC35 speckles or nuclear splicing speckles.
组织特异性 :
Detected in all tissues tested: skeletal muscle, heart, aorta, large intestine, small intestine, stomach, esophagus, bladder, adrenal gland, thyroid, pancreas, testis, adipose tissue, kidney, liver, spleen, cerebral cortex, blood serum and cerebrospinal fluid. Highest levels are found in the heart and in tissues composed of striated and smooth muscle.
蛋白名 :
热休克蛋白乙1
recommended detection systems :
Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P), IHC(F) and ICC.
sequence similarities :
Belongs to the small heat shock protein (HSP20) family.
更新 :
3/29/19 6:09
公司信息
武汉博士德生物工程有限公司
3942 B Valley Ave
Pleasanton, CA 94566
boster@bosterbio.com
http://www.boster.com.cn
925.485.4527
公司总部: 美国
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