产品简要
公司名称 :
武汉博士德生物工程有限公司
产品类型 :
抗体
产品名称 :
Anti-CD8 alpha/CD8A Antibody Picoband™
目录 :
PB9249
规格 :
100μg/vial
价格 :
315美元
克隆性 :
多克隆
宿主 :
domestic rabbit
共轭标签 :
未共轭
反应物种 :
人类
应用 :
免疫印迹, 免疫组化
图像
图像 1 :
武汉博士德生物工程有限公司 PB9249 图像 1
Anti- CD 8 alpha Picoband antibody, PB9249, Western blotting. All lanes: Anti CD 8 alpha (PB9249) at 0.5ug/ml. WB: Recombinant Human CD 8 alpha Protein 0.5ng. Predicted bind size: 29KD. Observed bind size: 29KD
图像 2 :
武汉博士德生物工程有限公司 PB9249 图像 2
Anti- CD 8 alpha Picoband antibody, PB9249, Western blotting. All lanes: Anti CD 8 alpha (PB9249) at 0.5ug/ml. Lane 1: JURKAT Whole Cell Lysate at 40ug. Lane 2: RAJI Whole Cell Lysate at 40ug. Lane 3: HL-60 Whole Cell Lysate at 40ug. Lane 4: A549 Whole Cell Lysate at 40ug. Lane 5: COLO320 Whole Cell Lysate at 40ug. Lane 6: HELA Whole Cell Lysate at 40ug. Predicted bind size: 26KD. Observed bind size: 55KD
图像 3 :
武汉博士德生物工程有限公司 PB9249 图像 3
Anti- CD 8 alpha Picoband antibody, PB9249, IHC(P). IHC(P): Human Tonsil Tissue
产品信息
SKU号 :
PB9249
产品名称 :
Anti-CD8 alpha/CD8A Antibody Picoband™
价格 :
315美元
规格 :
100μg/vial
克隆性 :
多克隆
宿主 :
反应物种 :
人类
应用(s) :
免疫组化, 免疫印迹
应用细节 :
Immunohistochemistry (Paraffin-embedded Section), 0.5-1µg/ml, Human, By Heat. Western blot, 0.1-0.5µg/ml, Human.
应用注释 :
WB: The detection limit for CD8 alpha is approximately 0.2ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.
描述 :
Boster Bio Anti-CD8 alpha/CD8A Antibody Picoband™ catalog # PB9249. Tested in IHC, WB applications. This antibody reacts with Human.
浓度 :
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
基因名称 :
CD8A
UniProt数据库编号 :
P01732
免疫原 :
E.coli-derived human CD8 alpha recombinant protein (Position: S22-A187). Human CD8 alpha shares 49% and 47% amino acid (aa) sequence identity with mouse and rat CD8 alpha, respectively.
形式 :
冻干
Contents :
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
纯化 :
免疫原亲和纯化
交叉活性 :
No cross reactivity with other proteins
储存 :
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
重组 :
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
基因全称 :
T细胞表面的糖蛋白CD8甲链
同义词 :
T-cell surface glycoprotein CD8 alpha chain; T-lymphocyte differentiation antigen T8/Leu-2; CD8a; CD8A; MAL;
蛋白名 :
T细胞表面的糖蛋白CD8甲链
分子量 :
25729分子量
Protein Function :
Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains.
Subcellular Localization :
Isoform 1: Cell membrane; Single-pass type I membrane protein.
Recommended Detection Systems :
Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
Sequence Similarities :
Contains 1 Ig-like V-type (immunoglobulin-like) domain.
背景 :
The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. It is mapped to 2p11.2. The CD8 antigen, acting as a coreceptor, and the T-cell receptor on the T lymphocyte recognize antigen displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The functional coreceptor is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. This gene also encodes the CD8 alpha chain isoforms.
研究种类 :
Adaptive Immunity, Immunology, T Cells
公司信息
武汉博士德生物工程有限公司
3942 B Valley Ave
Pleasanton, CA 94566
boster@bosterbio.com
http://www.boster.com.cn
925.485.4527
公司总部: 美国
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