产品简要
公司名称 :
武汉博士德生物工程有限公司
产品类型 :
抗体
产品名称 :
Anti-LRRK2 Antibody Picoband™
目录 :
PB9281
规格 :
100μg/vial
价格 :
315美元
克隆性 :
多克隆
宿主 :
domestic rabbit
共轭标签 :
未共轭
反应物种 :
人类, 小鼠, 大鼠
应用 :
免疫印迹
图像
图像 1 :
武汉博士德生物工程有限公司 PB9281 图像 1
Anti- LRRK2 Picoband antibody, PB9281, Western blotting. All lanes: Anti LRRK2 (PB9281) at 0.5ug/ml. Lane 1: Rat Brain Tissue Lysate at 50ug. Lane 2: Mouse Brain Tissue Lysate at 50ug. Lane 3: Rat Liver Tissue Lysate at 50ug. Lane 4: U87 Whole Cell Lysate at 40ug. Lane 5: NEURO Whole Cell Lysate at 40ug. Lane 6: A549 Whole Cell Lysate at 40ug. Lane 7: SMMC Whole Cell Lysate at 40ug. Predicted bind size: 286KD. Observed bind size: 286 KD
产品信息
SKU号 :
PB9281
产品名称 :
Anti-LRRK2 Antibody Picoband™
价格 :
315美元
规格 :
100μg/vial
克隆性 :
多克隆
宿主 :
反应物种 :
人类, 小鼠, 大鼠
应用(s) :
免疫印迹
应用细节 :
免疫印迹, 0.1 0.5µg/毫升, 人类, 小鼠, 大鼠
应用注释 :
WB: The detection limit for LRRK2 is approximately 0.25ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.
描述 :
Boster Bio Anti-LRRK2 Antibody Picoband™ catalog # PB9281. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.
浓度 :
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
基因名称 :
LRRK2
UniProt数据库编号 :
Q5S007
免疫原 :
E.coli-derived human LRRK2 recombinant protein (Position: E10-L350). Human LRRK2 shares 82% amino acid (aa) sequence identity with mouse LRRK2.
形式 :
冻干
Contents :
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
纯化 :
免疫原亲和纯化
交叉活性 :
No cross reactivity with other proteins
储存 :
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
重组 :
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
基因全称 :
Leucine-rich repeat serine/threonine-protein kinase 2
同义词 :
Leucine-rich repeat serine/threonine-protein kinase 2; 2.7.11.1; Dardarin; LRRK2; PARK8;
蛋白名 :
Leucine-rich repeat serine/threonine-protein kinase 2
分子量 :
286103分子量
Protein Function :
Positively regulates autophagy through a calcium- dependent activation of the CaMKK/AMPK signaling pathway. The process involves activation of nicotinic acid adenine dinucleotide phosphate (NAADP) receptors, increase in lysosomal pH, and calcium release from lysosomes. Together with RAB29, plays a role in the retrograde trafficking pathway for recycling proteins, such as mannose 6 phosphate receptor (M6PR), between lysosomes and the Golgi apparatus in a retromer-dependent manner. Regulates neuronal process morphology in the intact central nervous system (CNS). Plays a role in synaptic vesicle trafficking. Phosphorylates PRDX3. Has GTPase activity. May play a role in the phosphorylation of proteins central to Parkinson disease.
Subcellular Localization :
Membrane; Peripheral membrane protein. Cytoplasm. Perikaryon. Mitochondrion. Golgi apparatus. Cell projection, axon. Cell projection, dendrite. Endoplasmic reticulum. Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane ; Peripheral membrane protein ; Cytoplasmic side. Endosome. Lysosome. Mitochondrion outer membrane. Mitochondrion inner membrane. Mitochondrion matrix. Predominantly associated with intracytoplasmic vesicular and membranous structures (By similarity). Localized in the cytoplasm and associated with cellular membrane structures. Predominantly associated with the mitochondrial outer membrane of the mitochondria. Colocalized with RAB29 along tubular structures emerging from Golgi apparatus. Localizes in intracytoplasmic punctate structures of neuronal perikarya and dendritic and axonal processes.
组织特异性 :
Expressed in the brain. Expressed in pyramidal neurons in all cortical laminae of the visual cortex, in neurons of the substantia nigra pars compacta and caudate putamen (at protein level). Expressed throughout the adult brain, but at a lower level than in heart and liver. Also expressed in placenta, lung, skeletal muscle, kidney and pancreas. In the brain, expressed in the cerebellum, cerebral cortex, medulla, spinal cord occipital pole, frontal lobe, temporal lobe and putamen. Expression is particularly high in brain dopaminoceptive areas.
Recommended Detection Systems :
Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
Sequence Similarities :
Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.
背景 :
Leucine-rich repeat kinase 2 (LRRK2), also known as dardarin, is anenzyme that in humans is encoded by the PARK8 gene. It is a member of the leucine-rich repeat kinase family. This gene is mapped to 12q12. The protein is present largely in the cytoplasm but also associates with the mitochondrial outer membrane. Expression of mutant LRRK2 induced apoptotic cell death in neuroblastoma cells and in mouse cortical neurons. It has been determined that LRRK2 possesses mixed-lineage kinase activity, and LRRK2 also showed autophosphorylation activity. What’s more, LRRK2 has an affinity for lipids or lipid-associated proteins and may play a role in the biogenesis or regulation of membranous intracellular structures in the brain. It is also an IFNG target gene that may be involved in signaling pathways relevant to Crohn disease.
研究种类 :
Neurodegenerative Disease, Neurology Process, Neuroscience
公司信息
武汉博士德生物工程有限公司
3942 B Valley Ave
Pleasanton, CA 94566
boster@bosterbio.com
http://www.boster.com.cn
925.485.4527
公司总部: 美国
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