产品简要
公司名称 :
武汉博士德生物工程有限公司
产品类型 :
抗体
产品名称 :
Anti-Profilin 1/PFN1 Antibody Picoband™
目录 :
PB9313
规格 :
100μg/vial
价格 :
315美元
克隆性 :
多克隆
宿主 :
domestic rabbit
共轭标签 :
未共轭
反应物种 :
人类, 小鼠, 大鼠
应用 :
免疫印迹, 免疫细胞化学, 流式细胞仪, 免疫组化-石蜡切片
更多信息或购买 :
图像
图像 1 :
Anti- Profilin1 Picoband antibody, PB9313, Western blotting. All lanes: Anti Profilin1 (PB9313) at 0.5ug/ml. Lane 1: Rat Testis Tissue Lysate at 50ug. Lane 2: Mouse Testis Tissue Lysate at 50ug. Lane 3: Human Placenta Tissue Lysate at 50ug. Lane 4: PANC Whole Cell Lysate at 40ug. Lane 5: SW620 Whole Cell Lysate at 40ug. Predicted bind size: 15KD. Observed bind size: 15KD
图像 2 :
Figure 2. Flow Cytometry analysis of PC-3 cells using anti-Profilin1 antibody (PB9313). Overlay histogram showing PC-3 cells stained with PB9313 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti- Profilin1 Antibody (PB9313, 1ug/1x10 6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10ug/1x10 6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x10 6 ) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
产品信息
SKU号 :
PB9313
产品名称 :
Anti-Profilin 1/PFN1 Antibody Picoband™
价格 :
315美元
规格 :
100μg/vial
克隆性 :
多克隆
宿主 :
兔
反应物种 :
人类, 小鼠, 大鼠
前体活性 :
仓鼠
应用(s) :
流式细胞仪, IF, 免疫组化-P, 免疫细胞化学, 免疫印迹
应用细节 :
Western blot, 0.1-0.5µg/ml, Human, Mouse, Rat.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1µg/ml, Human, By Heat.
Immunocytochemistry/Immunofluorescence, 2µg/ml, Human.
Flow Cytometry, 1-3µg/1x10^6 cells, Human
应用注释 :
WB: The detection limit for Profilin 1 is approximately 0.1ng/lane under reducing conditions.
Tested Species: In-house tested species with positive results.
Other applications have not been tested.
Optimal dilutions should be determined by end users.
描述 :
Boster Bio Anti-Profilin 1/PFN1 Antibody Picoband™ catalog # PB9313. Tested in Flow Cytometry, IF, IHC-P, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.
浓度 :
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
基因名称 :
PFN1
UniProt数据库编号 :
P07737
免疫原 :
A synthetic peptide corresponding to a sequence at the C-terminus of human Profilin 1 (116-140aa KEGVHGGLINKKCYEMASHLRRSQY), identical to the related mouse and rat sequences.
形式 :
冻干
Contents :
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
纯化 :
免疫原亲和纯化
交叉活性 :
No cross reactivity with other proteins
储存 :
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
重组 :
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
基因全称 :
Profilin-1
同义词 :
Profilin-1; Epididymis tissue protein Li 184a; Profilin I; PFN1;
蛋白名 :
Profilin-1
分子量 :
15054分子量
Protein Function :
Binds to actin and affects the structure of the cytoskeleton. At high concentrations, profilin prevents the polymerization of actin, whereas it enhances it at low concentrations. By binding to PIP2, it inhibits the formation of IP3 and DG. Inhibits androgen receptor (AR) and HTT aggregation and binding of G-actin is essential for its inhibition of AR.
Subcellular Localization :
细胞质, 细胞骨架
组织特异性 :
Expressed in epididymis (at protein level).
Recommended Detection Systems :
Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, supported by SA1022 in IHC(P) and ICC.
背景 :
Profilin-1 is a protein that in humans is encoded by the PFN1 gene. The protein encoded by this gene is a ubiquitous actin monomer-binding protein belonging to the profilin family. PFN1 is mapped to 17p13.2. At high concentrations, profilin prevents the polymerization of actin, whereas it enhances it at low concentrations. This gene is a 140-amino acid protein and major growth regulator of filamentous (F)-actin through its binding of monomeric (G)-actin. It is thought to regulate actin polymerization in response to extracellular signals. Deletion of this gene is associated with Miller-Dieker syndrome.
研究种类 :
Actin, Actin Assembly, Actin Etc, Cancer, Cell Biology, Cell Cycle, Cell Differentiation, Cell Type Marker, Cytoskeleton, Cytoskeleton / Ecm, Microfilaments, Neuron Marker, Neuroscience, Oncoproteins/Suppressors, Signal Transduction, Subcellular Markers, Synapse Marker, Tags & Cell Markers, Tumor Suppressors
更多信息或购买 :
公司信息
武汉博士德生物工程有限公司
3942 B Valley Ave
Pleasanton, CA 94566
Pleasanton, CA 94566
boster@bosterbio.com
http://www.boster.com.cn925.485.4527
公司总部: 美国
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