产品简要
公司名称 :
武汉博士德生物工程有限公司
产品类型 :
抗体
产品名称 :
Anti-Apolipoprotein E/APOE Antibody Picoband™
目录 :
PB9327
规格 :
100μg/vial
价格 :
315美元
克隆性 :
多克隆
宿主 :
domestic rabbit
共轭标签 :
未共轭
反应物种 :
人类
应用 :
免疫印迹, 酶联免疫吸附测定, 流式细胞仪, 免疫组化-石蜡切片, 免疫组化-冰冻切片
图像
图像 1 :
武汉博士德生物工程有限公司 PB9327 图像 1
Figure 1. Western blot analysis of Apolipoprotein E using anti-Apolipoprotein E antibody (PB9327). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: Human Placenta Tissue Lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Apolipoprotein E antigen affinity purified polyclonal antibody (Catalog # PB9327) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Apolipoprotein E at approximately 36KD. The expected band size for Apolipoprotein E is at 36KD.
图像 2 :
武汉博士德生物工程有限公司 PB9327 图像 2
Figure 2. IHC analysis of Apolipoprotein E using anti-Apolipoprotein E antibody (PB9327). Apolipoprotein E was detected in paraffin-embedded section of Human Placenta Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-Apolipoprotein E Antibody (PB9327) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
图像 3 :
武汉博士德生物工程有限公司 PB9327 图像 3
Figure 3. Flow Cytometry analysis of HepG2 cells using anti- Apolipoprotein E antibody (PB9327). Overlay histogram showing HepG2 cells stained with PB9327 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Apolipoprotein E Antibody (PB9327,1ug/1x10 6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10ug/1x10 6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x10 6 ) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
产品信息
SKU号 :
PB9327
产品名称 :
Anti-Apolipoprotein E/APOE Antibody Picoband™
价格 :
315美元
规格 :
100μg/vial
克隆性 :
多克隆
宿主 :
反应物种 :
人类
应用(s) :
酶联免疫吸附测定, 流式细胞仪, 免疫组化-P, 免疫组化-F, 免疫印迹
应用细节 :
Western blot, 0.1-0.5µg/ml. Immunohistochemistry (Paraffin-embedded Section), 0.5-1µg/ml, By Heat. Immunohistochemistry (Frozen Section), 0.5-1µg/ml. Flow Cytometry, 1-3μg/1x10^6 cells. ELISA (Cap), 1-5µg/ml
应用注释 :
WB: The detection limit for Apolipoprotein E is approximately 0.1ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.
描述 :
Boster Bio Anti-Apolipoprotein E/APOE Antibody Picoband™ catalog # PB9327. Tested in ELISA, Flow Cytometry, IHC-P, IHC-F, WB applications. This antibody reacts with Human.
浓度 :
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
基因名称 :
ApoE基因
UniProt数据库编号 :
P02649
免疫原 :
E.coli-derived human Apolipoprotein E recombinant protein (Position: K19-H317). Human Apolipoprotein E shares 73% and 72% amino acid (aa) sequence identity with mouse and rat Apolipoprotein E, respectively.
形式 :
冻干
Contents :
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
纯化 :
免疫原亲和纯化
交叉活性 :
No cross reactivity with other proteins
储存 :
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
重组 :
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
基因全称 :
载脂蛋白e
同义词 :
载脂蛋白e;载脂蛋白E;APOE;
蛋白名 :
载脂蛋白e
分子量 :
36154分子量
Protein Function :
Mediates the binding, internalization, and catabolism of lipoprotein particles. It can serve as a ligand for the LDL (apo B/E) receptor and for the specific apo-E receptor (chylomicron remnant) of hepatic tissues.
Subcellular Localization :
Secreted.
组织特异性 :
Occurs in all lipoprotein fractions in plasma. It constitutes 10-20% of very low density lipoproteins (VLDL) and 1-2% of high density lipoproteins (HDL). APOE is produced in most organs. Significant quantities are produced in liver, brain, spleen, lung, adrenal, ovary, kidney and muscle.
Recommended Detection Systems :
Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P), IHC(F) and ICC.
Sequence Similarities :
Belongs to the apolipoprotein A1/A4/E family.
背景 :
APOE is also known as AD2 or LPG. The protein encoded by this gene is a major apoprotein of the chylomicron. It binds to a specific liver and peripheral cell receptor, and is essential for the normal catabolism of triglyceride-rich lipoprotein constituents. This gene maps to chromosome 19 in a cluster with the related apolipoprotein C1 and C2 genes. Mutations in this gene result in familial dysbetalipoproteinemia, or type III hyperlipoproteinemia (HLP III), in which increased plasma cholesterol and triglycerides are the consequence of impaired clearance of chylomicron and VLDL remnants. Alternative splicing results in multiple transcript variants.
研究种类 :
Cardiovascular, Lipid Metabolism, Lipids / Lipoproteins, Metabolism, Signal Transduction
公司信息
武汉博士德生物工程有限公司
3942 B Valley Ave
Pleasanton, CA 94566
boster@bosterbio.com
http://www.boster.com.cn
925.485.4527
公司总部: 美国
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