产品简要
公司名称 :
武汉博士德生物工程有限公司
产品类型 :
抗体
产品名称 :
Anti-MASPIN/SERPINB5 Picoband Antibody
目录 :
PB9356
规格 :
100 ug/vial
价格 :
280美元
克隆性 :
多克隆
宿主 :
共轭标签 :
nonconjugated
反应物种 :
人类, 小鼠, 大鼠
应用 :
免疫印迹, 免疫细胞化学, 免疫组化-石蜡切片
图像
图像 1 :
武汉博士德生物工程有限公司 PB9356 图像 1
Anti- MASPIN Picoband antibody, PB9356, Western blotting. All lanes: Anti MASPIN (PB9356) at 0.5ug/ml. Lane 1: Mouse Intestine Tissue Lysate at 50ug. Lane 2: Human Placenta Tissue Lysate at 50ug. Lane 3: MCF-7 Whole Cell Lysate at 40ug. Lane 4: MM231 Whole Cell Lysate at 40ug. Predicted bind size: 42KD. Observed bind size: 42KD
图像 2 :
武汉博士德生物工程有限公司 PB9356 图像 2
Anti- MASPIN Picoband antibody, PB9356, IHC(P). IHC(P): Mouse Intestine Tissue
图像 3 :
武汉博士德生物工程有限公司 PB9356 图像 3
Anti- MASPIN Picoband antibody, PB9356, IHC(P). IHC(P): Rat Intestine Tissue
产品信息
SKU号 :
PB9356
状态 :
Enabled
名称 :
Anti-MASPIN/SERPINB5 Picoband Antibody
目录名称 :
一抗, 多克隆抗体, 免疫组化免疫细胞化学IF抗体
基因名称 :
SERPINB5
价格 :
280美元
price various sizes :
Unconjugated / $280 APC / $330 APC-Cy7 / $330 FITC / $330 PE / $330 PE-Cy5 / $330 PE-Cy7 / $330 30ug sample size / $99 100ug / $280 100ug+Free HRP Secondary BA1054 / $280 100ug+Free Biotin Secondary BA1003 / $280
克隆性 :
多克隆
浓度 :
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
共轭标签 :
No
contents :
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
描述 :
Polyclonal antibody for Maspin/SERPINB5 detection. Host: Rabbit.Size: 100ug/vial. Tested applications: IHC-P. Reactive species: Human. Maspin/SERPINB5 information: Molecular Weight: 42100 MW; Subcellular Localization: Secreted, extracellular space; Tissue Specificity: Normal mammary epithelial cells.
short description :
Rabbit IgG polyclonal antibody for Serpin B5(SERPINB5) detection. Tested with WB, IHC-P, ICC in Human;Mouse;Rat.
规格 :
100 ug/vial
sample size available :
30ug for $99, contact us for details
UniProt数据库编号 :
P36952
宿主 :
免疫原 :
E.coli-derived human MASPIN recombinant protein (Position: M1-A350). Human MASPIN shares 88% and 89% amino acid (aa) sequence identity with mouse and rat MASPIN, respectively.
形式 :
冻干
纯化 :
免疫原亲和纯化
储存 :
At -20 C for one year. After reconstitution, at 4 C for one month. It can also be aliquotted and stored frozen at -20 C for a longer time.Avoid repeated freezing and thawing.
交叉活性 :
No cross reactivity with other proteins.
重组 :
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
应用细节 :
Immunocytochemistry , 0.5-1ug/ml, Human, -. Immunohistochemistry(Paraffin-embedded Section), 0.5-1ug/ml, Human, Mouse, Rat, By Heat. Western blot, 0.1-0.5ug/ml, Human, Mouse, Rat.
应用 :
免疫组化-P, 免疫细胞化学, 免疫印迹
反应物种 :
人类, 小鼠, 大鼠
图像标记 :
Anti- MASPIN Picoband antibody, PB9356, Western blotting. All lanes: Anti MASPIN (PB9356) at 0.5ug/ml. Lane 1: Mouse Intestine Tissue Lysate at 50ug. Lane 2: Human Placenta Tissue Lysate at 50ug. Lane 3: MCF-7 Whole Cell Lysate at 40ug. Lane 4: MM231 Whole Cell Lysate at 40ug. Predicted bind size: 42KD. Observed bind size: 42KD Anti- MASPIN Picoband antibody, PB9356, IHC(P). IHC(P): Mouse Intestine Tissue Anti- MASPIN Picoband antibody, PB9356, IHC(P). IHC(P): Rat Intestine Tissue Anti- MASPIN Picoband antibody, PB9356, IHC(P). IHC(P): Human Mammary Cancer Tissue Anti- MASPIN Picoband antibody, PB9356, ICC. ICC: MCF-7 Cell Figure 6. Western blot analysis of MASPIN using anti- MASPIN antibody (PB9356). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat small intestine tissue lysates, . Lane 2: mouse small intestine tissue lysates, . Lane 3: human placenta tissue lysates, . Lane 4: human MDA-MB-231 whole cell lysates, . Lane 5: human MCF-7 whole cell lysates, . After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti- MASPIN antigen affinity purified polyclonal antibody (Catalog # PB9356) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MASPIN at approximately 42KD. The expected band size for MASPIN is at 42KD.
背景 :
SERPINB5 is also known as PI5 or maspin. Maspin (mammary serine protease inhibitor) is a protein that in humans is encoded by the SERPINB5 gene. Maspin is expressed in the skin, prostate, testis, intestine, tongue, lung, and the thymus. Maspin is a member of the serpin superfamily of serine protease inhibitors.[1] The primary function of most members of this family is to regulate the breakdown of proteins by inhibiting the catalytic activity of proteinases. Through this mechanism of action, serpins regulate a number of cellular processes includingphagocytosis, coagulation, and fibrinolysis.
competitor equivalent skus :
sc 54036 sc 54035 sc 134793 sc 54034 sc 54033 sc 393352 sc 393338
研究种类 :
Cancer, Cell Type Markers, Oncoproteins/Suppressors, Tags & Cell Markers, Tumor Associated, Tumor Suppressors
同义词 :
Serpin B5;Maspin;Peptidase inhibitor 5;PI-5;SERPINB5;PI5;
基因全称 :
丝氨酸蛋白酶抑制剂B5
分子量 :
42100分子量
protein function :
Tumor suppressor. It blocks the growth, invasion, and metastatic properties of mammary tumors. As it does not undergo the S (stressed) to R (relaxed) conformational transition characteristic of active serpins, it exhibits no serine protease inhibitory activity.
subcellular localization :
Secreted, extracellular space.
组织特异性 :
Normal mammary epithelial cells.
蛋白名 :
丝氨酸蛋白酶抑制剂B5
recommended detection systems :
Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P) and ICC.
sequence similarities :
Belongs to the serpin family. Ov-serpin subfamily.
更新 :
4/15/19 9:16
公司信息
武汉博士德生物工程有限公司
3942 B Valley Ave
Pleasanton, CA 94566
boster@bosterbio.com
http://www.boster.com.cn
925.485.4527
公司总部: 美国
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