产品简要
公司名称 :
武汉博士德生物工程有限公司
产品类型 :
抗体
产品名称 :
Anti-Vimentin Antibody Picoband™
目录 :
PB9359
规格 :
100μg/vial
价格 :
315美元
克隆性 :
多克隆
宿主 :
domestic rabbit
共轭标签 :
未共轭
反应物种 :
人类, 小鼠, 大鼠
应用 :
免疫印迹, 免疫组化
图像
图像 1 :
武汉博士德生物工程有限公司 PB9359 图像 1
Anti- Vimentin Picoband antibody, PB9359, Western blotting. All lanes: Anti Vimentin (PB9359) at 0.5ug/ml. Lane 1: HT1080 Whole Cell Lysate at 40ug. Lane 2: NIH Whole Cell Lysate at 40ug. Lane 3: JURKAT Whole Cell Lysate at 40ug. Lane 4: HUT Whole Cell Lysate at 40ug. Lane 5: MCF-7 Whole Cell Lysate at 40ug. Lane 6: HELA Whole Cell Lysate at 40ug. Lane 7: Human Placenta Tissue Lysate at 50ug. Lane 8: Rat Testis Tissue Lysate at 50ug. Lane 9: Mouse Testis Tissue Lysate at 50ug. Predicted bind size: 54KD. Observed bind size: 54KD
图像 2 :
武汉博士德生物工程有限公司 PB9359 图像 2
Anti- Vimentin Picoband antibody, PB9359, IHC(P). IHC(P): Mouse Cardiac Muscle Tissue
图像 3 :
武汉博士德生物工程有限公司 PB9359 图像 3
Anti- Vimentin Picoband antibody, PB9359, IHC(P). IHC(P): Rat Cardiac Muscle Tissue
产品信息
SKU号 :
PB9359
产品名称 :
Anti-Vimentin Antibody Picoband™
价格 :
315美元
规格 :
100μg/vial
克隆性 :
多克隆
宿主 :
反应物种 :
人类, 小鼠, 大鼠
前体活性 :
仓鼠
应用(s) :
免疫组化, 免疫印迹
应用细节 :
Immunohistochemistry (Paraffin-embedded Section), 0.5-1µg/ml, Human, Mouse, Rat, By Heat. Western blot, 0.1-0.5µg/ml, Human, Mouse, Rat.
应用注释 :
WB: The detection limit for Vimentin is approximately 0.1ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.
描述 :
Boster Bio Anti-Vimentin Antibody Picoband™ catalog # PB9359. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.
浓度 :
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
基因名称 :
弹性蛋白
UniProt数据库编号 :
P08670
免疫原 :
A synthetic peptide corresponding to a sequence at the C-terminus of human Vimentin (435-466aa DTHSKRTLLIKTVETRDGQVINETSQHHDDLE), identical to the related mouse and rat sequences.
形式 :
冻干
Contents :
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
纯化 :
免疫原亲和纯化
交叉活性 :
No cross reactivity with other proteins.
储存 :
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
重组 :
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
基因全称 :
弹性蛋白
同义词 :
弹性蛋白;弹性蛋白;
蛋白名 :
弹性蛋白
分子量 :
53652分子量
Protein Function :
Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.
Subcellular Localization :
细胞质
组织特异性 :
Highly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone- independent mammary carcinoma cell lines.
Recommended Detection Systems :
Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
Sequence Similarities :
Belongs to the intermediate filament family.
背景 :
VIM (vimentin) is also known as HEL113 or CTRCT30. This gene encodes a member of the intermediate filament family. Intermediate filamentents, along with microtubules and actin microfilaments, make up the cytoskeleton. The protein encoded by this gene is responsible for maintaining cell shape, integrity of the cytoplasm, and stabilizing cytoskeletal interactions. It is also involved in the immune response, and controls the transport of low-density lipoprotein (LDL)-derived cholesterol from a lysosome to the site of esterification. It functions as an organizer of a number of critical proteins involved in attachment, migration, and cell signaling. Mutations in this gene causes a dominant, pulverulent cataract.
研究种类 :
Cancer, Cell Type Marker, Cytoskeleton, Cytoskeleton / Ecm, Developmental Biology, Intermediate Filaments, Intracellular, Lineage Markers, Lineage Specification, Neural Stem Cells, Neurogenesis, Neurology Process, Neuroscience, Signal Transduction, Stem Cells, Tumor Biomarkers
公司信息
武汉博士德生物工程有限公司
3942 B Valley Ave
Pleasanton, CA 94566
boster@bosterbio.com
http://www.boster.com.cn
925.485.4527
公司总部: 美国
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