产品简要
公司名称 :
武汉博士德生物工程有限公司
产品类型 :
抗体
产品名称 :
Anti-Plectin Antibody Picoband™
目录 :
PB9430
规格 :
100μg/vial
价格 :
315美元
克隆性 :
多克隆
宿主 :
domestic rabbit
共轭标签 :
未共轭
反应物种 :
人类, 小鼠, 大鼠
应用 :
免疫印迹, 免疫细胞化学, 流式细胞仪, 免疫组化-石蜡切片
图像
图像 1 :
武汉博士德生物工程有限公司 PB9430 图像 1
Anti- Plectin Picoband antibody, PB9430, Western blotting. All lanes: Anti Plectin (PB9430) at 0.5ug/ml. Lane 1: HELA Whole Cell Lysate at 40ug. Lane 2: Rat Brain Tissue Lysate at 50ug. Predicted bind size: 532KD. Observed bind size: 532KD
图像 2 :
武汉博士德生物工程有限公司 PB9430 图像 2
Anti- Plectin Picoband antibody, PB9430, IHC(P). IHC(P): Mouse Intestine Tissue
图像 3 :
武汉博士德生物工程有限公司 PB9430 图像 3
Anti- Plectin Picoband antibody, PB9430, IHC(P). IHC(P): Rat Intestine Tissue
产品信息
SKU号 :
PB9430
产品名称 :
Anti-Plectin Antibody Picoband™
价格 :
315美元
规格 :
100μg/vial
克隆性 :
多克隆
宿主 :
反应物种 :
人类, 小鼠, 大鼠
前体活性 :
仓鼠
应用(s) :
流式细胞仪, IF, 免疫组化-P, 免疫细胞化学, 免疫印迹
应用细节 :
Western blot, 0.1-0.5µg/ml, Human, Rat. Immunohistochemistry (Paraffin-embedded Section), 0.5-1µg/ml, Human, Mouse, Rat, By Heat. Immunocytochemistry/Immunofluorescence, 2µg/ml, Human. Flow Cytometry, 1-3μg/1x10^6 cells, Human
应用注释 :
WB: The detection limit for Plectin is approximately 0.1ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.
描述 :
Boster Bio Anti-Plectin Antibody Picoband™ catalog # PB9430. Tested in Flow Cytometry, IF, IHC-P, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.
浓度 :
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
基因名称 :
PLEC
UniProt数据库编号 :
Q15149
免疫原 :
A synthetic peptide corresponding to a sequence in the middle region of human Plectin (2644-2671aa RFIEQEKAKLEQLFQDEVAKAQQLREEQ), different from the related mouse sequence by one amino acid, and from the related rat sequence by two amino acids.
形式 :
冻干
Contents :
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
纯化 :
免疫原亲和纯化
交叉活性 :
No cross reactivity with other proteins
储存 :
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
重组 :
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
基因全称 :
Plectin
同义词 :
Plectin; PCN; PLTN; Hemidesmosomal protein 1; HD1; Plectin-1; PLEC; PLEC1;
蛋白名 :
Plectin
分子量 :
531791分子量
Protein Function :
Interlinks intermediate filaments with microtubules and microfilaments and anchors intermediate filaments to desmosomes or hemidesmosomes. Could also bind muscle proteins such as actin to membrane complexes in muscle. May be involved not only in the filaments network, but also in the regulation of their dynamics. Structural component of muscle. Isoform 9 plays a major role in the maintenance of myofibers integrity.
Subcellular Localization :
Cytoplasm, cytoskeleton. Cell junction, hemidesmosome.
组织特异性 :
Widely expressed with highest levels in muscle, heart, placenta and spinal cord.
Recommended Detection Systems :
Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P) and ICC.
Sequence Similarities :
Belongs to the plakin or cytolinker family.
背景 :
Plectin, known as PLEC, is a prominent member of an important family of structurally and in part functionally related proteins, termed plakins or cytolinkers, that are capable of interlinking different elements of the cytoskeleton. Plakins, with their multi-domain structure and enormous size, not only play crucial roles in maintaining cell and tissue integrity and orchestrating dynamic changes in cytoarchitecture and cell shape, but also serve as scaffolding platforms for the assembly, positioning, and regulation of signaling complexes. Plectin is expressed as several protein isoforms in a wide range of cell types and tissues from a single gene located on chromosome 8 in humans. The plectin gene locus in mouse on chromosome 15 has been analyzed in detail, revealing a genomic exon-intron organization with well over 40 exons spanning over 62 kb and an unusual 5' transcript complexity of plectin isoforms. Eleven exons (1-1j) have been identified that alternatively splice directly into a common exon 2 which is the first exon to encode plectin's highly conserved actin binding domain (ABD). Three additional exons (-1, 0a, and 0) splice into an alternative first coding exon (1c), and two additional exons (2alpha and 3alpha) are optionally spliced within the exons encoding the acting binding domain (exons 2-8). Analysis of the human locus has identified eight of the eleven alternative 5' exons found in mouse and rat; exons 1i, 1j and 1h have not been confirmed in human. Furthermore, isoforms lacking the central rod domain encoded by exon 31 have been detected in mouse, rat, and human. The short alternative amino-terminal sequences encoded by the different first exons direct the targeting of the various isoforms to distinct subcellular locations. As the expression of specific plectin isoforms was found to be dependent on cell type (tissue) and stage of development, it appears that each cell type (tissue) contains a unique set (proportion and composition) of plectin isoforms, as if custom-made for specific requirements of the particular cells. Concordantly, individual isoforms were found to carry out distinct and specific functions.
研究种类 :
Cytoskeleton, Cytoskeleton / Ecm, Intermediate Filaments, Signal Transduction
公司信息
武汉博士德生物工程有限公司
3942 B Valley Ave
Pleasanton, CA 94566
boster@bosterbio.com
http://www.boster.com.cn
925.485.4527
公司总部: 美国
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