产品简要
公司名称 :
武汉博士德生物工程有限公司
产品类型 :
抗体
产品名称 :
Anti-CD55 Antibody Picoband™
目录 :
PB9487
规格 :
100μg/vial
价格 :
315美元
克隆性 :
多克隆
宿主 :
domestic rabbit
共轭标签 :
未共轭
反应物种 :
人类
应用 :
免疫印迹, 免疫细胞化学, 流式细胞仪, 免疫组化-石蜡切片
更多信息或购买 :
图像
图像 1 :
Figure 1. Western blot analysis of CD55 using anti-CD55 antibody (PB9487). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: K562 Whole Cell Lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD55 antigen affinity purified polyclonal antibody (Catalog # PB9487) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD55 at approximately 41KD. The expected band size for CD55 is at 41KD.
图像 2 :
Figure 2. IHC analysis of CD55 using anti-CD55 antibody (PB9487). CD55 was detected in paraffin-embedded section of Human Placenta Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-CD55 Antibody (PB9487) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
图像 3 :
Figure 3. Flow Cytometry analysis of PC-3 cells using anti- CD55 antibody (PB9487). Overlay histogram showing PC-3 cells stained with PB9487 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CD55 Antibody (PB9487,1ug/1x10 6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10ug/1x10 6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x10 6 ) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
产品信息
SKU号 :
PB9487
产品名称 :
Anti-CD55 Antibody Picoband™
价格 :
315美元
规格 :
100μg/vial
克隆性 :
多克隆
宿主 :
兔
反应物种 :
人类
应用(s) :
流式细胞仪, IF, 免疫组化-P, 免疫细胞化学, 免疫印迹
应用细节 :
Western blot, 0.1-0.5µg/ml, Human.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1µg/ml, Human, By Heat.
Immunocytochemistry/Immunofluorescence, 2µg/ml, Human.
Immunofluorescence, 2µg/ml, Human.
Flow Cytometry, 1-3µg/1x10^6 cells, Human
应用注释 :
Tested Species: In-house tested species with positive results.
By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections.
Other applications have not been tested.
Optimal dilutions should be determined by end users.
描述 :
Boster Bio Anti-CD55 Antibody Picoband™ catalog # PB9487. Tested in Flow Cytometry, IF, IHC-P, ICC, WB applications. This antibody reacts with Human.
浓度 :
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
基因名称 :
CD55
UniProt数据库编号 :
P08174
免疫原 :
E.coli-derived human CD55 recombinant protein (Position: D35-K347). Human CD55 shares 49.1% amino acid (aa) sequence identity with mouse CD55.
形式 :
冻干
Contents :
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
纯化 :
免疫原亲和纯化
交叉活性 :
No cross reactivity with other proteins
储存 :
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
重组 :
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
基因全称 :
Complement decay-accelerating factor
同义词 :
Complement decay-accelerating factor; CD55; CD55; CR, DAF;
蛋白名 :
Complement decay-accelerating factor
分子量 :
41400分子量
Protein Function :
This protein recognizes C4b and C3b fragments that condense with cell-surface hydroxyl or amino groups when nascent C4b and C3b are locally generated during C4 and c3 activation. Interaction of daf with cell-associated C4b and C3b polypeptides interferes with their ability to catalyze the conversion of C2 and factor B to enzymatically active C2a and Bb and thereby prevents the formation of C4b2a and C3bBb, the amplification convertases of the complement cascade.
Subcellular Localization :
Isoform 1: Cell membrane; Single-pass type I membrane protein.
组织特异性 :
Expressed on the plasma membranes of all cell types that are in intimate contact with plasma complement proteins. It is also found on the surfaces of epithelial cells lining extracellular compartments, and variants of the molecule are present in body fluids and in extracellular matrix.
Recommended Detection Systems :
Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P) and ICC.
Sequence Similarities :
Belongs to the receptors of complement activation (RCA) family.
背景 :
Complement decay-accelerating factor, also known as CD55 or DAF, is a protein that, in humans, is encoded by the CD55 gene. This gene encodes a glycoprotein involved in the regulation of the complement cascade. Binding of the encoded protein to complement proteins accelerates their decay, thereby disrupting the cascade and preventing damage to host cells. Antigens present on this protein constitute the Cromer blood group system (CROM). Alternative splicing results in multiple transcript variants. The predominant transcript variant encodes a membrane-bound protein, but alternatively spliced transcripts may produce soluble proteins.
研究种类 :
细胞类型标记, 免疫学
更多信息或购买 :
公司信息
武汉博士德生物工程有限公司
3942 B Valley Ave
Pleasanton, CA 94566
Pleasanton, CA 94566
boster@bosterbio.com
http://www.boster.com.cn925.485.4527
公司总部: 美国
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