抗体

Anti-KIN Antibody Picoband™

PB9607

100μg/vial

315美元

多克隆

domestic rabbit

未共轭

人类, 小鼠

免疫印迹, 免疫组化

Anti-KIN Antibody Picoband™

315美元

100μg/vial

多克隆

兔

人类, 小鼠

免疫组化, 免疫印迹

Immunohistochemistry (Paraffin-embedded Section), 0.5-1µg/ml, Human, By Heat. Western blot, 0.1-0.5µg/ml, Human, Mouse.

Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Boster Bio Anti-KIN Antibody Picoband™ catalog # PB9607. Tested in IHC, WB applications. This antibody reacts with Human, Mouse.

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

KIN

O60870

E.coli-derived human KIN recombinant protein (Position: K3-K202). Human KIN shares 98% amino acid (aa) sequence identity with mouse KIN.

冻干

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

免疫原亲和纯化

No cross reactivity with other proteins

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

脱氧核糖核酸/核糖核酸结合蛋白KIN17

DNA/RNA-binding protein KIN17; Binding to curved DNA; KIN, antigenic determinant of recA protein homolog; KIN ; BTCD, KIN17;

脱氧核糖核酸/核糖核酸结合蛋白KIN17

45374分子量

Involved in DNA replication and the cellular response to DNA damage. May participate in DNA replication factories and create a bridge between DNA replication and repair mediated by high molecular weight complexes. May play a role in illegitimate recombination and regulation of gene expression. May participate in mRNA processing. Binds, in vitro, to double-stranded DNA. Also shown to bind preferentially to curved DNA in vitro and in vivo (By similarity). Binds via its C-terminal domain to RNA in vitro.

Nucleus. Cytoplasm. During S phase, strongly associated with the nuclear matrix, and to chromosomal DNA in the presence of DNA damage. Also shows cytoplasmic localization in elongated spermatids.

Ubiquitously expressed in all tissues examined, with highest levels in skeletal muscle, heart and testis. Differentially expressed in non-tumorigenic and tumorigenic cell lines. Highly expressed in proliferating epithelial keratinocyte cells in vitro (at protein level).

Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).

Belongs to the KIN17 family.

DNA/RNA-binding protein KIN17, also known as BTCD or KIN17, is a protein that in humans is encoded by the KIN gene. This gene is mapped to 10p14. The protein encoded by this gene is a nuclear protein that forms intranuclear foci during proliferation and is redistributed in the nucleoplasm during the cell cycle. Short-wave ultraviolet light provokes the relocalization of the protein, suggesting its participation in the cellular response to DNA damage. Originally selected based on protein-binding with RecA antibodies, the mouse protein presents a limited similarity with a functional domain of the bacterial RecA protein, a characteristic shared by this human ortholog. Alternative splicing of this gene results in multiple transcript variants.

DNA / RNA, DNA Damage & Repair, DNA Damage Response, Epigenetics and Nuclear Signaling