产品简要
公司名称 :
武汉博士德生物工程有限公司
产品类型 :
抗体
产品名称 :
Anti-Integrin alpha 4/ITGA4 Picoband Antibody
目录 :
PB9648
规格 :
100 ug/vial
价格 :
280美元
克隆性 :
多克隆
宿主 :
共轭标签 :
nonconjugated
反应物种 :
人类, 大鼠
应用 :
免疫印迹, 流式细胞仪, 免疫组化-石蜡切片, 免疫组化-冰冻切片
图像
图像 1 :
武汉博士德生物工程有限公司 PB9648 图像 1
Anti- Integrin alpha 4 Picoband antibody, PB9648, Western blotting. All lanes: Anti Integrin alpha 4 (PB9648) at 0.5ug/ml. Lane 1: Rat Liver Tissue Lysate at 50ug. Lane 2: Rat Kidney Tissue Lysate at 50ug. Lane 3: JURKAT Whole Cell Lysate at 40ug. Lane 4: 22RV1 Whole Cell Lysate at 40ug. Lane 5: HEPG2 Whole Cell Lysate at 40ug. Lane 6: SMMC Whole Cell Lysate at 40ug. Predicted bind size: 115KD. Observed bind size: 220KD
图像 2 :
武汉博士德生物工程有限公司 PB9648 图像 2
Anti- Integrin alpha 4 Picoband antibody, PB9648, IHC(P). IHC(P): Human Mammary Cancer Tissue
图像 3 :
武汉博士德生物工程有限公司 PB9648 图像 3
Figure 3. IHC analysis of Integrin alpha 4 using anti- Integrin alpha 4 antibody (PB9648). Integrin alpha 4 was detected in frozen section of human placenta tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti- Integrin alpha 4 Antibody (PB9648) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
产品信息
SKU号 :
PB9648
状态 :
Enabled
名称 :
Anti-Integrin alpha 4/ITGA4 Picoband Antibody
目录名称 :
一抗, 多克隆抗体, 免疫组化免疫细胞化学IF抗体
基因名称 :
ITGA4
价格 :
280美元
price various sizes :
Unconjugated / $280 APC / $330 APC-Cy7 / $330 FITC / $330 PE / $330 PE-Cy5 / $330 PE-Cy7 / $330 30ug sample size / $99 100ug / $280 100ug+Free HRP Secondary BA1054 / $280 100ug+Free Biotin Secondary BA1003 / $280
克隆性 :
多克隆
浓度 :
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
共轭标签 :
No
contents :
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
描述 :
Polyclonal antibody for CD49d/ITGA4 detection. Host: Rabbit.Size: 100ug/vial. Tested applications: WB, IHC-P, IHC-F, FCM. Reactive species: Human. CD49d/ITGA4 information: Molecular Weight: 114900 MW; Subcellular Localization: Membrane; Single-pass type I membrane protein.
short description :
Rabbit IgG polyclonal antibody for Integrin alpha-4(ITGA4) detection. Tested with WB, IHC-P, IHC-F, FCM in Human;Rat.
规格 :
100 ug/vial
sample size available :
30ug for $99, contact us for details
UniProt数据库编号 :
P13612
宿主 :
免疫原 :
A synthetic peptide corresponding to a sequence at the C-terminus of human Integrin alpha 4 (999-1028aa MWKAGFFKRQYKSILQEENRRDSWSYINS K), different from the related mouse sequence by one amino acid.
形式 :
冻干
纯化 :
免疫原亲和纯化
储存 :
At -20 C for one year. After reconstitution, at 4 C for one month. It can also be aliquotted and stored frozen at -20 C for a longer time.Avoid repeated freezing and thawing.
交叉活性 :
No cross reactivity with other proteins
重组 :
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
应用细节 :
Western blot, 0.1-0.5ug/ml, Human, Rat. Immunohistochemistry(Paraffin-embedded Section), 0.5-1ug/ml, Human, By Heat. Immunohistochemistry(Frozen Section), 0.5-1ug/ml, Human. Flow Cytometry, 1-3ug/1x10 6 cells, Human
应用 :
流式细胞仪, 免疫组化-P, 免疫组化-F, 免疫印迹
反应物种 :
人类, 大鼠
图像标记 :
Anti- Integrin alpha 4 Picoband antibody, PB9648, Western blotting. All lanes: Anti Integrin alpha 4 (PB9648) at 0.5ug/ml. Lane 1: Rat Liver Tissue Lysate at 50ug. Lane 2: Rat Kidney Tissue Lysate at 50ug. Lane 3: JURKAT Whole Cell Lysate at 40ug. Lane 4: 22RV1 Whole Cell Lysate at 40ug. Lane 5: HEPG2 Whole Cell Lysate at 40ug. Lane 6: SMMC Whole Cell Lysate at 40ug. Predicted bind size: 115KD. Observed bind size: 220KD Anti- Integrin alpha 4 Picoband antibody, PB9648, IHC(P). IHC(P): Human Mammary Cancer Tissue Figure 3. IHC analysis of Integrin alpha 4 using anti- Integrin alpha 4 antibody (PB9648). Integrin alpha 4 was detected in frozen section of human placenta tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti- Integrin alpha 4 Antibody (PB9648) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. Figure 4. Flow Cytometry analysis of THP-1 cells using anti- Integrin alpha 4 antibody (PB9648). Overlay histogram showing THP-1 cells stained with PB9648 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti- Integrin alpha 4 Antibody (PB9648,1ug/1x10 6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10ug/1x10 6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x10 6 ) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
背景 :
ITGA4/Integrin alpha4 is an integrin alpha subunit. It makes up half of the alpha4beta1 lymphocyte homing receptor. The product of this gene belongs to the integrin alpha chain family of proteins. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This gene encodes an alpha 4 chain. Unlike other integrin alpha chains, alpha 4 neither contains an I-domain, nor undergoes disulfide-linked cleavage. Alpha 4 chain associates with either beta 1 chain or beta 7 chain. ITGA4 is also mapped to 2q31-q32 by fluorescence in situ hybridization.
competitor equivalent skus :
sc 14008 sc 23933 sc 23943 sc 365209 sc 365569 sc 376334 sc 52593 sc 6589 sc 6589 R sc 6590
研究种类 :
Cell Adhesion, Cell Type Markers, Cytoskeleton / Ecm, Immunology, Integrins, Neural Stem Cells, Signal Transduction, Stem Cells, Surface Molecules
同义词 :
Integrin alpha-4;CD49 antigen-like family member D;Integrin alpha-IV;VLA-4 subunit alpha;CD49d;ITGA4;CD49D;
基因全称 :
整合素甲4
分子量 :
114900分子量
protein function :
Integrins alpha-4/beta-1 (VLA-4) and alpha-4/beta-7 are receptors for fibronectin. They recognize one or more domains within the alternatively spliced CS-1 and CS-5 regions of fibronectin. They are also receptors for VCAM1. Integrin alpha- 4/beta-1 recognizes the sequence Q-I-D-S in VCAM1. Integrin alpha- 4/beta-7 is also a receptor for MADCAM1. It recognizes the sequence L-D-T in MADCAM1. On activated endothelial cells integrin VLA-4 triggers homotypic aggregation for most VLA-4-positive leukocyte cell lines. It may also participate in cytolytic T-cell interactions with target cells.
subcellular localization :
Membrane; Single-pass type I membrane protein.
蛋白名 :
整合素甲4
recommended detection systems :
Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P) and IHC(F).
更新 :
4/25/19 7:30
公司信息
武汉博士德生物工程有限公司
3942 B Valley Ave
Pleasanton, CA 94566
boster@bosterbio.com
http://www.boster.com.cn
925.485.4527
公司总部: 美国
Premium Provider of Antibodies and ELISA Kits