产品简要
公司名称 :
武汉博士德生物工程有限公司
产品类型 :
抗体
产品名称 :
Anti-Kv1.2/KCNA2 Antibody Picoband‚Ñ¢
目录 :
PB9649
规格 :
10微克样本
价格 :
99美元
克隆性 :
多克隆
宿主 :
domestic rabbit
共轭标签 :
未共轭
反应物种 :
人类, 小鼠, 大鼠
应用 :
免疫印迹, 免疫细胞化学, 流式细胞仪
图像
图像 1 :
武汉博士德生物工程有限公司 PB9649 图像 1
Anti- Kv1.2 Picoband antibody, PB9649, Western blotting. All lanes: Anti Kv1.2 (PB9649) at 0.5ug/ml. Lane 1: Rat Kidney Tissue Lysate at 50ug. Lane 2: Rat Brain Tissue Lysate at 50ug. Lane 3: Mouse Brain Tissue Lysate at 50ug. Lane 4: Mouse Kidney Tissue Lysate at 50ug. Predicted bind size: 57KD. Observed bind size: 57KD
产品信息
SKU号 :
PB9649
状态 :
1
名称 :
Anti-Kv1.2/KCNA2 Antibody Picoband‚Ñ¢
url key :
抗kv1-2
产品种类 :
一抗
基因名称 :
KCNA2
价格 :
315
规格1 :
10微克样本
price for size 1 :
99美元
规格2 :
Full size
price for size 2 :
315
规格3 :
Full size carrier free
price for size 3 :
365
克隆性 :
多克隆
浓度 :
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
contents :
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
描述 :
Boster Bio Anti-Kv1.2/KCNA2 Antibody Picoband catalog # PB9649. Tested in Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.
short description :
Boster Bio Anti-Kv1.2/KCNA2 Antibody Picoband catalog # PB9649. Tested in Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.
规格 :
100μg/vial
UniProt数据库编号 :
P16389
宿主 :
免疫原 :
A synthetic peptide corresponding to a sequence at the C-terminus of human Kv1.2 (466-499aa NNSNEDFREENLKTANCTLANTNYVNITKMLTDV), identical to the related mouse and rat sequences.
形式 :
冻干
纯化 :
免疫原亲和纯化
储存 :
Store at -20ÀöC for one year from date of receipt. After reconstitution, at 4ÀöC for one month. It can also be aliquotted and stored frozen at -20ÀöC for six months. Avoid repeated freeze-thaw cycles.
交叉活性 :
No cross reactivity with other proteins
重组 :
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
应用细节 :
免疫印迹, 0.1 0.5微克/毫升, 小鼠, 大鼠。 免疫细胞化学/免疫荧光, 2微克/毫升, 人类。 流式细胞仪, 1-3Œºg/1x10 6细胞, 人类, 大鼠
应用 :
流式细胞仪, IF, 免疫细胞化学, 免疫印迹
反应物种 :
人类, 小鼠, 大鼠
前体活性 :
仓鼠
images :
/antibody/pb9649-1-WB-anti-kv1-2-picoband-antibody.jpg /p/b/pb9649-2.jpg /p/b/pb9649-3.jpg /p/b/pb9649-4.jpg
图像标记 :
Figure 1. Western blot analysis of Kv1.2 using anti-Kv1.2 antibody (PB9649). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Rat Kidney Tissue Lysate at 50ug,. Lane 2: Rat Brain Tissue Lysate at 50ug,. Lane 3: Mouse Brain Tissue Lysate at 50ug,. Lane 4: Mouse Kidney Tissue Lysate at 50ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Kv1.2 antigen affinity purified polyclonal antibody (Catalog # PB9649) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Kv1.2 at approximately 57 kDa. The expected band size for Kv1.2 is at 57 kDa. Figure 2. IF analysis of Kv1.2 using anti-Kv1.2 antibody (PB9649). Kv1.2 was detected in immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-Kv1.2 Antibody (PB9649) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. Figure 3. Flow Cytometry analysis of A431 cells using anti-Kv1.2 antibody (PB9649). Overlay histogram showing A431 cells stained with PB9649 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Kv1.2 Antibody (PB9649,1μg/1x10 6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10 6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10 6 ) used under the same conditions. Unlabelled sample (Red line) was also used as a control. Figure 4. Flow Cytometry analysis of C6 cells using anti-Kv1.2 antibody (PB9649). Overlay histogram showing C6 cells stained with PB9649 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Kv1.2 Antibody (PB9649,1μg/1x10 6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10 6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10 6 ) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
custom options :
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created at :
9/24/15 20:41
updated at :
3/2/22 2:55
meta title :
Anti-Kv1.2/KCNA2 Antibody Picoband‚Ñ¢ Bosterbio
meta keyword :
Anti-Kv1.2/KCNA2 Antibody Picoband
meta description :
Boster Bio Anti-Kv1.2/KCNA2 Antibody Picoband catalog # PB9649. Tested in Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. Supplied as 100ug/vial in Lyophilized form antibody.
应用注释 :
Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.
rating value :
97.6
背景 :
Potassium voltage-gated channel subfamily A member 2, also known as Kv1.2, is a protein that in humans is encoded by the KCNA2 gene. Potassium channels represent the most complex class of voltage-gated ion channels from both functional and structural standpoints. Their diverse functions include regulating neurotransmitter release, heart rate, insulin secretion, neuronal excitability, epithelial electrolyte transport, smooth muscle contraction, and cell volume. Four sequence-related potassium channel genes - shaker, shaw, shab, and shal - have been identified in Drosophila, and each has been shown to have human homolog (s). This gene encodes a member of the potassium channel, voltage-gated, shaker-related subfamily. This member contains six membrane-spanning domains with a shaker-type repeat in the fourth segment. It belongs to the delayed rectifier class, members of which allow nerve cells to efficiently repolarize following an action potential. The coding region of this gene is intronless, and the gene is clustered with genes KCNA3 and KCNA10 on chromosome 1.
competitor equivalent skus :
sc 1734 sc 25608 sc 1736 sc 19984
研究种类 :
Neuroscience,Neurotransmission,Potassium Channels,Receptors / Channels
同义词 :
Potassium voltage-gated channel subfamily A member 2;NGK1;Voltage-gated K (+) channel HuKIV ;Voltage-gated potassium channel HBK5;Voltage-gated potassium channel subunit Kv1.2;KCNA2;
基因全称 :
Potassium voltage-gated channel subfamily A member 2
分子量 :
56717分子量
protein function :
Voltage-gated potassium channel that mediates transmembrane potassium transport in excitable membranes, primarily in the brain and the central nervous system, but also in the cardiovascular system. Prevents aberrant action potential firing and regulates neuronal output. Forms tetrameric potassium- selective channels through which potassium ions pass in accordance with their electrochemical gradient. The channel alternates between opened and closed conformations in response to the voltage difference across the membrane (PubMed:19912772, PubMed:8495559, PubMed:11211111, PubMed:23769686). Can form functional homotetrameric channels and heterotetrameric channels that contain variable proportions of KCNA1, KCNA2, KCNA4, KCNA5, KCNA6, KCNA7, and possibly other family members as well; channel properties depend on the type of alpha subunits that are part of the channel (PubMed:8495559, PubMed:20220134). Channel properties are modulated by cytoplasmic beta subunits that regulate the subcellular location of the alpha subunits and promote rapid inactivation of delayed rectifier potassium channels. In vivo, membranes probably contain a mixture of heteromeric potassium channel complexes, making it difficult to assign currents observed in intact tissues to any particular potassium channel family member. Homotetrameric KCNA2 forms a delayed-rectifier potassium channel that opens in response to membrane depolarization, followed by slow spontaneous channel closure (PubMed:19912772, PubMed:23769686). In contrast, a heteromultimer formed by KCNA2 and KCNA4 shows rapid inactivation (PubMed:8495559). Regulates neuronal excitability and plays a role as pacemaker in the regulation of neuronal action potentials (By similarity). KCNA2- containing channels play a presynaptic role and prevent hyperexcitability and aberrant action potential firing (By similarity). Response to toxins that are selective for KCNA2- containing potassium channels suggests that in Purkinje cells, dendritic subthreshold KCNA2-containing potassium channels prevent random spontaneous calcium spikes, suppressing dendritic hyperexcitability without hindering the generation of somatic action potentials, and thereby play an important role in motor coordination (By similarity). Plays a role in the induction of long-term potentiation of neuron excitability in the CA3 layer of the hippocampus (By similarity). May function as down-stream effector for G protein-coupled receptors and inhibit GABAergic inputs to basolateral amygdala neurons (By similarity). May contribute to the regulation of neurotransmitter release, such as gamma-aminobutyric acid (GABA) (By similarity). Contributes to the regulation of the axonal release of the neurotransmitter dopamine (By similarity). Reduced KCNA2 expression plays a role in the perception of neuropathic pain after peripheral nerve injury, but not acute pain (By similarity). Plays a role in the regulation of the time spent in non-rapid eye movement (NREM) sleep (By similarity).
subcellular localization :
Cell membrane ; Multi- pass membrane protein . Membrane . Cell projection, axon . Cell junction, synapse . Endoplasmic reticulum membrane . Cell projection, lamellipodium membrane . Cell junction, synapse, synaptosome . Cell junction, synapse, presynaptic cell membrane . Cell projection, dendrite . KCNA2 by itself is detected both at the endoplasmic reticulum and at the cell membrane. Coexpression with KCNA4 or with beta subunits promotes expression at the cell membrane. Coexpression with KCNA1 inhibits cell surface expression.
组织特异性 :
Detected in brain cortex (PubMed:16473933). Detected in peroneal nerve in the juxtaparanodal regions of the node of Ranvier; expression is decreased in patients with diabetes mellitus that suffer from axonal neuropathy (PubMed:22649228). Detected in paranodal and juxtanodal zones in myelinated spinal cord (at protein level) (PubMed:11086297).
蛋白名 :
Potassium voltage-gated channel subfamily A member 2
recommended detection systems :
Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for ICC.
template :
抗体
公司信息
武汉博士德生物工程有限公司
3942 B Valley Ave
Pleasanton, CA 94566
boster@bosterbio.com
http://www.boster.com.cn
925.485.4527
公司总部: 美国
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