产品简要
公司名称 :
武汉博士德生物工程有限公司
产品类型 :
抗体
产品名称 :
Anti-BCA1/CXCL13 Picoband Antibody
目录 :
PB9697
规格 :
100 ug/vial
价格 :
240美元
克隆性 :
多克隆
宿主 :
共轭标签 :
nonconjugated
反应物种 :
人类
应用 :
免疫印迹, 酶联免疫吸附测定
图像
图像 1 :
武汉博士德生物工程有限公司 PB9697 图像 1
Figure 1. Western blot analysis of CXCL13 using anti- CXCL13 antibody (PB9697). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane : HELA whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti- CXCL13 antigen affinity purified polyclonal antibody (Catalog # PB9697) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CXCL13 at approximately 13KD. The expected band size for CXCL13 is at 13KD.
产品信息
SKU号 :
PB9697
状态 :
Enabled
名称 :
Anti-BCA1/CXCL13 Picoband Antibody
目录名称 :
一抗, 多克隆抗体
基因名称 :
CXCL13
价格 :
240美元
price various sizes :
30ug sample size / $99 100ug / $240 100ug+Free HRP Secondary BA1054 / $240 100ug+Free Biotin Secondary BA1003 / $240
克隆性 :
多克隆
浓度 :
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
共轭标签 :
No
contents :
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
描述 :
Polyclonal antibody for BCA1/CXCL13 detection. Host: Rabbit.Size: 100ug/vial. Tested applications: ELISA. Reactive species: Human. BCA1/CXCL13 information: Molecular Weight: 12664 MW; Subcellular Localization: Secreted; Tissue Specificity: Highest levels in liver, followed by spleen, lymph node, appendix and stomach. Low levels in salivary gland, mammary gland and fetal spleen.
short description :
Rabbit IgG polyclonal antibody for C-X-C motif chemokine 13(CXCL13) detection. Tested with WB, ELISA(Cap) in Human.
规格 :
100 ug/vial
sample size available :
30ug for $99, contact us for details
UniProt数据库编号 :
O43927
宿主 :
免疫原 :
E. coli-derived human BCA1 recombinant protein (Position: V23-P109). Human BCA1 shares 45.3% amino acid (aa) sequence identity with mouse BCA1.
形式 :
冻干
纯化 :
免疫原亲和纯化
储存 :
At -20 C for one year. After reconstitution, at 4 C for one month. It can also be aliquotted and stored frozen at -20 C for a longer time.Avoid repeated freezing and thawing.
交叉活性 :
No cross reactivity with other proteins
重组 :
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
应用细节 :
ELISA(Cap), 1-5ug/ml, Human, -. Western blot, 0.1-0.5ug/ml, Human.
应用 :
酶联免疫吸附测定, 免疫印迹
反应物种 :
人类
图像标记 :
Figure 1. Western blot analysis of CXCL13 using anti- CXCL13 antibody (PB9697). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane : HELA whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti- CXCL13 antigen affinity purified polyclonal antibody (Catalog # PB9697) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CXCL13 at approximately 13KD. The expected band size for CXCL13 is at 13KD.
背景 :
C-X-C motif chemokine 13 (CXCL13), also known as B lymphocyte chemoattractant (BLC), is a protein that in humans is encoded by the CXCL13 gene. CXCL13 is a small cytokine belonging to the CXC chemokine family. Its gene is mapped to human chromosome 4 in a cluster of other CXC chemokines. This protein has contained 109 amino acids. It is expressed highly in the liver, spleen, lymph nodes and gut of humans. The protein induces chemotaxis of BLR1-expressing B cells.
competitor equivalent skus :
sc 323510 sc 73740
同义词 :
C-X-C motif chemokine 13;Angie;B cell-attracting chemokine 1;BCA-1;B lymphocyte chemoattractant;CXC chemokine BLC;Small-inducible cytokine B13;CXCL13;BCA1, BLC, SCYB13;
基因全称 :
C-X-C motif chemokine 13
分子量 :
12664分子量
protein function :
Chemotactic for B-lymphocytes but not for T-lymphocytes, monocytes and neutrophils. Does not induce calcium release in B- lymphocytes. Binds to BLR1/CXCR5.
subcellular localization :
Secreted.
组织特异性 :
Highest levels in liver, followed by spleen, lymph node, appendix and stomach. Low levels in salivary gland, mammary gland and fetal spleen.
蛋白名 :
C-X-C motif chemokine 13
recommended detection systems :
Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
更新 :
4/10/19 1:04
公司信息
武汉博士德生物工程有限公司
3942 B Valley Ave
Pleasanton, CA 94566
boster@bosterbio.com
http://www.boster.com.cn
925.485.4527
公司总部: 美国
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