抗体

Anti-CD27 Antibody Picoband™

PB9931

100μg/vial

315美元

多克隆

domestic rabbit

未共轭

人类, 小鼠

免疫印迹, 免疫细胞化学, 流式细胞仪, 免疫组化-石蜡切片

Anti-CD27 Antibody Picoband™

315美元

100μg/vial

多克隆

兔

人类, 小鼠

流式细胞仪, IF, 免疫组化-P, 免疫细胞化学, 免疫印迹

Western blot, 0.1-0.5µg/ml, Human, Mouse. Immunohistochemistry (Paraffin-embedded Section), 0.5-1µg/ml, Human, By Heat. Immunocytochemistry/Immunofluorescence, 2µg/ml, Human. Flow Cytometry, 1-3μg/1x10^6 cells, Human.

Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Boster Bio Anti-CD27 Antibody Picoband™ catalog # PB9931. Tested in Flow Cytometry, IF, IHC-P, ICC, WB applications. This antibody reacts with Human, Mouse.

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

CD27

P26842

E. coli-derived human CD27 recombinant protein (Position: A20-R191). Human CD27 shares 62.8% amino acid (aa) sequence identity with mouse CD27.

冻干

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

免疫原亲和纯化

No cross reactivity with other proteins

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

CD27抗原

CD27 antigen; CD27L receptor; T-cell activation antigen CD27; T14; Tumor necrosis factor receptor superfamily member 7; CD27; CD27; TNFRSF7;

CD27抗原

29137分子量

Receptor for CD70/CD27L. May play a role in survival of activated T-cells. May play a role in apoptosis through association with SIVA1.

Membrane; Single-pass type I membrane protein.

Found in most T-lymphocytes.

Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P) and ICC.

CD27 is a member of the tumor necrosis factor receptor superfamily. This receptor is required for generation and long-term maintenance of T cell immunity. It binds to ligand CD70, and plays a key role in regulating B-cell activation and immunoglobulin synthesis. And this receptor transduces signals that lead to the activation of NF-kappaB and MAPK8/JNK. Adaptor proteins TRAF2 and TRAF5 have been shown to mediate the signaling process of this receptor. CD27-binding protein (SIVA), a proapoptotic protein, can bind to this receptor and is thought to play an important role in the apoptosis induced by this receptor.

Adaptive Immunity, Cancer, Cytokines, Hematopoietic Progenitors, Immunology, Innate Immunity, Lymphoid, Stem Cells, T Cells, T Lymphocytic Lineage, Tnf Superfamily, Tumor Immunology