抗体

抗胱硫醚β合酶CBS抗体

RP1041

100μg/vial

315美元

多克隆

domestic rabbit

未共轭

人类, 大鼠

免疫印迹, 免疫组化

抗胱硫醚β合酶CBS抗体

315美元

100μg/vial

多克隆

兔

人类, 大鼠

免疫组化, 免疫印迹

Immunohistochemistry (Paraffin-embedded Section), 0.5-1µg/ml, Human, By Heat. Western blot, 0.1-0.5µg/ml, Human, Rat.

WB: The detection limit for CBS is approximately 0.25ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Boster Bio Anti-Cystathionine beta-synthase CBS Antibody catalog # RP1041. Tested in IHC, WB applications. This antibody reacts with Human, Rat.

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

CBS

P35520

E.coli-derived human CBS recombinant protein (Position: A331-K551). Human CBS shares 83% amino acid (aa) sequence identity with both mouse and rat CBS.

冻干

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

免疫原亲和纯化

No cross reactivity with other proteins

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

胱硫醚β合酶

Cystathionine beta-synthase; 4.2.1.22; Beta-thionase; Serine sulfhydrase; CBS;

胱硫醚β合酶

60587分子量

Only known pyridoxal phosphate-dependent enzyme that contains heme. Important regulator of hydrogen sulfide, especially in the brain, utilizing cysteine instead of serine to catalyze the formation of hydrogen sulfide. Hydrogen sulfide is a gastratransmitter with signaling and cytoprotective effects such as acting as a neuromodulator in the brain to protect neurons against hypoxic injury (By similarity).

Cytoplasm. Nucleus.

In the adult strongly expressed in liver and pancreas, some expression in heart and brain, weak expression in lung and kidney. In the fetus, expressed in brain, liver and kidney.

Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).

Belongs to the cysteine synthase/cystathionine beta- synthase family.

Cystathionine-β-synthase, also known as CBS, is an enzyme that in humans is encoded by the CBS gene. It is mapped to 21q22.3 and contains 23 exons, ranging in size from 42 to 299 bp. CBS catalyzes the first step of the transsulfuration pathway, from homocysteine to cystathionine. It uses the cofactor pyridoxal-phosphate (PLP) and can be allosterically regulated by effectors such as the ubiquitous cofactor S-adenosyl-L-methionine (adoMet). This enzyme belongs to the family of lyases, to be specific, the hydro-lyases, which cleave carbon-oxygen bonds. CBS is a multidomain enzyme composed of an N-terminal enzymatic domain and two CBS domains. The CBS gene is the most common locus for mutations associated with homocystinuria.

Amino Acid Metabolism, Amino Acids, Cancer, Cancer Metabolism, Metabolic Signaling Pathways, Metabolism, Pathways And Processes, Signal Transduction