抗体

抗水闸蛋白2/CLDN2抗体

RP1042

100μg/vial

315美元

多克隆

domestic rabbit

未共轭

人类, 大鼠

免疫印迹, 免疫组化

抗水闸蛋白2/CLDN2抗体

315美元

100μg/vial

多克隆

兔

人类, 大鼠

免疫组化, 免疫印迹

Immunohistochemistry (Paraffin-embedded Section), 0.5-1µg/ml, Human, By Heat. Western blot, 0.1-0.5µg/ml, Rat, Human.

WB: The detection limit for Claudin 2 is approximately 0.2ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Boster Bio Anti-Claudin 2/CLDN2 Antibody catalog # RP1042. Tested in IHC, WB applications. This antibody reacts with Human, Rat.

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

CLDN2

P57739

E.coli-derived human Claudin 2 recombinant protein (Position: A38-V230). Human Claudin 2 shares 92% amino acid (aa) sequence identity with mouse Claudin 2.

冻干

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

免疫原亲和纯化

No cross reactivity with other proteins

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

水闸蛋白2

水闸蛋白2;SP82;CLDN2;PSEC0059, SP82, UNQ705/PRO1356;

水闸蛋白2

24549分子量

Plays a major role in tight junction-specific obliteration of the intercellular space, through calcium- independent cell-adhesion activity.

Cell junction, tight junction. Cell membrane ; Multi-pass membrane protein.

Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).

Claudin-2 is a protein that in humans is encoded by the CLDN2 gene. By genomic sequence analysis, this gene is mapped to chromosome Xq22.3-q23. This gene product belongs to the claudin protein family whose members have been identified as major integral membrane proteins localized exclusively at tight junctions. Claudins are expressed in an organ-specific manner and regulate tissue-specific physiologic properties of tight junctions. By expression in a human intestinal epithelial cell line, it was determined that the intestine-specific homeodomain proteins CDX1 and CDX2 activated a reporter plasmid driven by the CLDN2 promoter.

Cell Adhesion, Cytoskeleton / Ecm, Signal Transduction, Tight Junctions