抗体

抗KAT13A/SRC1/NCOA1抗体

RP1056

100μg/vial

315美元

多克隆

domestic rabbit

未共轭

免疫印迹, 免疫细胞化学, 流式细胞仪

抗KAT13A/SRC1/NCOA1抗体

315美元

100μg/vial

多克隆

兔

人类

流式细胞仪, IF, 免疫细胞化学, 免疫印迹

免疫印迹, 0.1 0.5µg/毫升, 人类。 免疫细胞化学/免疫荧光, 2µg/毫升, 人类。 流式细胞仪, 1-3μg/1x10^6细胞, 人类

Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Boster Bio Anti-KAT13A/SRC1/NCOA1 Antibody catalog # RP1056. Tested in Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human.

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

NCOA1

Q15788

E.coli-derived human KAT13A recombinant protein (Position: H614-Q826). Human KAT13A shares 92% amino acid (aa) sequence identity with mouse KAT13A.

冻干

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

免疫原亲和纯化

No cross reactivity with other proteins

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Nuclear receptor coactivator 1

Nuclear receptor coactivator 1; NCoA-1; 2.3.1.48; Class E basic helix-loop-helix protein 74; bHLHe74; Protein Hin-2; RIP160; Renal carcinoma antigen NY-REN-52; Steroid receptor coactivator 1; SRC-1; NCOA1; BHLHE74, SRC1;

Nuclear receptor coactivator 1

156757分子量

Nuclear receptor coactivator that directly binds nuclear receptors and stimulates the transcriptional activities in a hormone-dependent fashion. Involved in the coactivation of different nuclear receptors, such as for steroids (PGR, GR and ER), retinoids (RXRs), thyroid hormone (TRs) and prostanoids (PPARs). Also involved in coactivation mediated by STAT3, STAT5A, STAT5B and STAT6 transcription factors. Displays histone acetyltransferase activity toward H3 and H4; the relevance of such activity remains however unclear. Plays a central role in creating multisubunit coactivator complexes that act via remodeling of chromatin, and possibly acts by participating in both chromatin remodeling and recruitment of general transcription factors. Required with NCOA2 to control energy balance between white and brown adipose tissues. Required for mediating steroid hormone response. Isoform 2 has a higher thyroid hormone-dependent transactivation activity than isoform 1 and isoform 3.

Nucleus.

Widely expressed.

Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for ICC.

Belongs to the SRC/p160 nuclear receptor coactivator family.

The nuclear receptor coactivator 1 (NCOA1), also known as SRC1, is a transcriptional coregulatory protein that contains several nuclear receptor interacting domains and an intrinsic histone acetyltransferase activity. NCOA1 is recruited to DNA promotion sites by ligand-activated nuclear receptors. NCOA1, in turn, acylates histones, which makes downsteam DNA more accessible to transcription. Hence, NCOA1 assists nuclear receptors in the upregulation of DNA expression. It has been found that NCOA1 can enhance the transcriptional activity of ligand-bound PGR but does not alter the basal activity of the target promoter. It also enhances estrogen receptor, glucocorticoid receptor, thyroid hormone receptor, and retinoid X receptor transcriptional activities through their cognate DNA response elements in the presence of hormone. What’s more, SRC1 may play a role as a bridging molecule between nuclear hormone receptors and general transcription factors.

Acetylation, Cancer, Cancer Metabolism, ChIP’ing Antibodies, Chromatin Modifying Enzymes, Co-Activators/Co-Repressors, Domain Families, Epigenetics and Nuclear Signaling, Hlh / Leucine Zipper, Lipid And Lipoprotein Metabolism, Lipid Metabolism, Metabolic Signaling Pathway, Metabolic Signaling Pathways, Metabolism, Metabolism Of Lipids And Lipoproteins, Nuclear Hormone Receptors, Nuclear Receptors, Nuclear Signaling Pathways, Oncoproteins, Oncoproteins/Suppressors, Pathways And Processes, Signal Transduction, Signaling Pathway, Transcription