产品简要
公司名称 :
武汉博士德生物工程有限公司
产品类型 :
抗体
产品名称 :
抗EAAT2/SLC1A2抗体
目录 :
RP1065
规格 :
100 ug/vial
价格 :
240美元
克隆性 :
多克隆
宿主 :
共轭标签 :
nonconjugated
反应物种 :
人类, 小鼠, 大鼠
应用 :
免疫印迹
图像
图像 1 :
武汉博士德生物工程有限公司 RP1065 图像 1
Figure 1. Western blot analysis of EAAT2 using anti-EAAT2 antibody (RP1065). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, . Lane 2: mouse brain tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EAAT2 antigen affinity purified polyclonal antibody (Catalog # RP1065) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EAAT2 at approximately 62KD. The expected band size for EAAT2 is at 62KD.
产品信息
SKU号 :
RP1065
状态 :
Enabled
名称 :
抗EAAT2/SLC1A2抗体
目录名称 :
一抗, 多克隆抗体
基因名称 :
SLC1A2
价格 :
240美元
price various sizes :
30ug sample size / $99 100ug / $240 100ug+Free HRP Secondary BA1054 / $240 100ug+Free Biotin Secondary BA1003 / $240
克隆性 :
多克隆
浓度 :
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
共轭标签 :
No
contents :
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
描述 :
Polyclonal antibody for EAAT2/SLC1A2 detection. Host: Rabbit.Size: 100ug/vial. Tested applications: WB. Reactive species: Human. EAAT2/SLC1A2 information: Molecular Weight: 62104 MW; Subcellular Localization: Membrane; Multi-pass membrane protein.
short description :
Rabbit IgG polyclonal antibody for Excitatory amino acid transporter 2(SLC1A2) detection. Tested with WB in Human;Mouse;Rat.
规格 :
100 ug/vial
sample size available :
30ug for $99, contact us for details
UniProt数据库编号 :
P43004
宿主 :
免疫原 :
E.coli-derived human EAAT2 recombinant protein (Position: T461-K574). Human EAAT2 shares 96% amino acid (aa) sequence identity with both mouse and rat EAAT2.
形式 :
冻干
纯化 :
免疫原亲和纯化
储存 :
At -20 C for one year. After reconstitution, at 4 C for one month. It can also be aliquotted and stored frozen at -20 C for a longer time.Avoid repeated freezing and thawing.
交叉活性 :
No cross reactivity with other proteins
重组 :
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
应用细节 :
免疫印迹, 0.1 0.5微克/毫升, 人类, 小鼠, 大鼠
应用 :
免疫印迹
反应物种 :
人类, 小鼠, 大鼠
图像标记 :
Figure 1. Western blot analysis of EAAT2 using anti-EAAT2 antibody (RP1065). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, . Lane 2: mouse brain tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EAAT2 antigen affinity purified polyclonal antibody (Catalog # RP1065) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EAAT2 at approximately 62KD. The expected band size for EAAT2 is at 62KD.
背景 :
SLC1A2 is also known as EAAT2 or GLT-1. This gene encodes a member of a family of solute transporter proteins. The membrane-bound protein is the principal transporter that clears the excitatory neurotransmitter glutamate from the extracellular space at synapses in the central nervous system. Glutamate clearance is necessary for proper synaptic activation and to prevent neuronal damage from excessive activation of glutamate receptors. Mutations in and decreased expression of this protein are associated with amyotrophic lateral sclerosis. Alternatively spliced transcript variants of this gene have been identified.
competitor equivalent skus :
sc 135892 sc 15317 sc 31581 sc 31582 sc 365634 sc 7760
研究种类 :
神经科学, 神经递质, 信号转导, 转运蛋白
同义词 :
Excitatory amino acid transporter 2;Glutamate/aspartate transporter II;Sodium-dependent glutamate/aspartate transporter 2;Solute carrier family 1 member 2;SLC1A2;EAAT2, GLT1;
基因全称 :
兴奋性氨基酸转运体2
分子量 :
62104分子量
protein function :
Transports L-glutamate and also L- and D-aspartate. Essential for terminating the postsynaptic action of glutamate by rapidly removing released glutamate from the synaptic cleft. Acts as a symport by cotransporting sodium.
subcellular localization :
Membrane; Multi-pass membrane protein.
蛋白名 :
兴奋性氨基酸转运体2
recommended detection systems :
Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
更新 :
3/15/19 3:44
公司信息
武汉博士德生物工程有限公司
3942 B Valley Ave
Pleasanton, CA 94566
boster@bosterbio.com
http://www.boster.com.cn
925.485.4527
公司总部: 美国
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