蛋白

Recombinant Human Replication Protein A2, 32kDa, His-tagged

RPA2-2852H

Recombinant Human Replication Protein A2, 32kDa, His-tagged

Creative Biomart

RPA2

replication protein A2, 32kDa

RPA2; replication protein A2, 32kDa; replication protein A2 (32kD); replication protein A 32 kDa subunit; RP-A p32; RP-A p34; RF-A protein 2; replication factor A protein 2; replication protein A 34 kDa subunit; REPA2; RPA32

Recombinant human RPA2 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. MW =31.7 kDa (293aa) confirmed by MALDI-TOF.

Recombinant human RPA2 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. MW =31.7 kDa (293aa) confirmed by MALDI-TOF.Replication Protein A (RPA) is a single stranded DNA binding protein. Human RPA is a heterotrimeric protein containing subunits of 14, 32 and 70kDa. This protein complex is highly conserved in eukaryotes and is essential in DNA replication, homologous recombination and nucleotide excision repair. The C-terminus of RPA2 can specfically intereact with the DNA repair enzyme UNG2 and repair factors XPA and Rad52, each of which functions in a different repair pathway. In addition, this protein binds specifically to the SH2 domain of Stat3 in vivo, and overexpression of RPA2 corresponds to the augmented growth factor-stimulated tyrosine phosphorylation and transcription activities of Stat3.

Ecoli

液体。 在20mM三羟甲基氨基甲烷氯化氢缓冲液(pH值8.0)含10% 甘油, 2mM DTT, 0.1M氯化钠

85 % by SDS – PAGE.

0.5毫克/毫升(由布拉德福德Bradford检测确定)

可以储存在+4 C短期(1-2周)。 为长期储存, 分成小份儿保存在-20 C或-70 C。 避免反复冻融

P15927