产品简要
公司名称 :
Developmental Studies Hybridoma Bank
产品类型 :
抗体
产品名称 :
CD44
目录 :
5D2-27
克隆性 :
单克隆
宿主 :
大鼠
共轭标签 :
未共轭
克隆名称 :
5D2-27
反应物种 :
小鼠
应用 :
免疫印迹, 酶联免疫吸附测定, 免疫组化, 免疫细胞化学, 免疫沉淀, 流式细胞仪, 免疫印迹基因敲除验证
更多信息或购买 :
Developmental Studies Hybridoma Bank 产品网页
文章摘录数: 8
出版应用/物种/样本/稀释参考文献
  • 免疫印迹基因敲除验证; 小鼠; 图 2b
Freeman S, Vega A, Riedl M, Collins R, Ostrowski P, Woods E, et al. Transmembrane Pickets Connect Cyto- and Pericellular Skeletons Forming Barriers to Receptor Engagement. Cell. 2018;172:305-317.e10 pubmed 出版商
Caceres P, Gravotta D, Zager P, Dephoure N, Rodriguez Boulan E. Quantitative proteomics of MDCK cells identify unrecognized roles of clathrin adaptor AP-1 in polarized distribution of surface proteins. Proc Natl Acad Sci U S A. 2019;116:11796-11805 pubmed 出版商
Lewis J, Schwartz M. Mapping in vivo associations of cytoplasmic proteins with integrin beta 1 cytoplasmic domain mutants. Mol Biol Cell. 1995;6:151-60 pubmed
Hughes E, August J. Characterization of plasma membrane proteins identified by monoclonal antibodies. J Biol Chem. 1981;256:664-71 pubmed
Hughes E, Mengod G, August J. Murine cell surface glycoproteins. Characterization of a major component of 80,000 daltons as a polymorphic differentiation antigen of mesenchymal cells. J Biol Chem. 1981;256:7023-7 pubmed
Murphy T, Decker G, August J. Glycoproteins of coated pits, cell junctions, and the entire cell surface revealed by monoclonal antibodies and immunoelectron microscopy. J Cell Biol. 1983;97:533-41 pubmed
Jacobson K, O DELL D, August J. Lateral diffusion of an 80,000-dalton glycoprotein in the plasma membrane of murine fibroblasts: relationships to cell structure and function. J Cell Biol. 1984;99:1624-33 pubmed
Jacobson K, O DELL D, Holifield B, Murphy T, August J. Redistribution of a major cell surface glycoprotein during cell movement. J Cell Biol. 1984;99:1613-23 pubmed
产品信息
内部号码 :
468
名称 :
5D2-27
Depositor Name :
August, J.T.
Depositor Institution :
Johns Hopkins University School of Medicine
Depositor Website :
https://www.hopkinsmedicine.org/pharmacology_molecular_sciences/faculty/bios/august.html
Date Deposited :
8/1/86
Allow Hybridoma Distribution :
No
Cells Available (legacy) :
No
抗原 :
CD44
抗原物种 :
小鼠
宿主物种 :
大鼠
抗体亚型 :
RIgG2a
Isotype for catalog (legacy) :
IgG2a
Positive Tested Species Reactivity :
小鼠
Species Tested (legacy) :
specific to mouse
Initial Publication Pubmed ID :
7451466
Depositor Notes (Special Instructions) :
This antigen was originally called Pgp-1 (Ly-24) lymphocyte cell adhesion glycoprotein and the antibody was identified as AMF-15. The 5D2-27 mAb is specific for mouse CD44 only.
Collections :
CD antigens,Cell adhesion,Cell markers,Cell signaling,Extracellular matrix proteins
搜索关键词 :
August, J.T., CD44, Mouse, RIgG2a, Mouse, CD44, Ly-24, AB_2076440, Monoclonal , Cell markers/Cell adhesion/Cluster Determinant antigens, Immunoprecipitation/Western blot/ELISA/FACS/Immunofluorescence/Immunohistochemistry
抗原分子量 :
80kDa
基因 :
CD44
Alternate Gene Name(s) :
Ly-24
UniProt数据库编号 :
P15379
抗体登记处号码 :
AB_2076440
Synonyms (Alternate Clone Names) :
CD44
免疫原 :
NIH 3T3成纤维细胞血浆膜
免疫原序列 :
全长蛋白
克隆性 :
单克隆
Myeloma Strain :
P3-NSI/1-Ag4-1
表位定位 :
No
Recommended Applications :
酶联免疫吸附测定, 流式细胞仪, 免疫荧光, 免疫组化, 免疫沉淀, 免疫印迹
Immunoblotting (legacy) :
Yes
免疫组化PubMed IDs :
6411735
免疫荧光PubMed IDs :
6386823 6386824
免疫沉淀PubMed IDs :
7451466 6386823 6787057
酶联免疫吸附测定PubMed IDs :
7451466
流式细胞仪PubMed IDs :
7540435 6787057
其他信息 :
RRID:AB_2076440
DSHB增长培养基 :
Iscove's
References (legacy) :
J. Biol. Chem. 256, 664-671.; J. Biol. Chem. 256, 7023-7027.; Arch. Biochem. Biophys. 209, 718-722.; Proc. Natl. Acad. Sci. USA 79, 1926-1929.; J. Biol. Chem. 258, 1014-1021.; J. Cell Biol. 97, 533-541.; J. Cell Biol. 99, 1613-1623.; J. Cell Biol. 99, 1624-1633.; J. Tiss. Cult. Meth. 14, 31-36.
更多信息或购买 :
Developmental Studies Hybridoma Bank 产品网页
公司信息
Developmental Studies Hybridoma Bank
University of Iowa
dshb@uiowa.edu
http://dshb.biology.uiowa.edu
公司总部: 美国
Developmental Studies Hybridoma Bank (DSHB) 是NIH于1996年创办的国家机构，为生命科学研究领域及诊断领域，提供高质量的抗体等产品。