产品简要
公司名称 :
Developmental Studies Hybridoma Bank
产品类型 :
抗体
产品名称 :
sonic hedgehog
目录 :
5E1
克隆性 :
单克隆
宿主 :
小鼠
共轭标签 :
未共轭
克隆名称 :
5E1
反应物种 :
common quail, Gallotia galloti, 人类, 小鼠, 大鼠, 鸡
应用 :
免疫印迹, 免疫组化, 免疫细胞化学, 流式细胞仪, 免疫组化-石蜡切片, 免疫组化基因敲除验证
更多信息或购买 :
Developmental Studies Hybridoma Bank 产品网页
文章摘录数: 46
出版应用/物种/样本/稀释参考文献
  • 免疫组化基因敲除验证; 小鼠; 1:20; 图 s8b
  • 免疫组化-石蜡切片; 小鼠; 1:20; 图 6b
He H, Huang M, Sun S, Wu Y, Lin X. Epithelial heparan sulfate regulates Sonic Hedgehog signaling in lung development. PLoS Genet. 2017;13:e1006992 pubmed 出版商
  • 免疫组化; 鸡; 1:200; 图 s2
Amarnath S, Agarwala S. Cell-cycle-dependent TGF?-BMP antagonism regulates neural tube closure by modulating tight junctions. J Cell Sci. 2017;130:119-131 pubmed 出版商
  • 免疫组化基因敲除验证; common quail; 图 6c
Matsubara Y, Nakano M, Kawamura K, Tsudzuki M, Funahashi J, Agata K, et al. Inactivation of Sonic Hedgehog Signaling and Polydactyly in Limbs of Hereditary Multiple Malformation, a Novel Type of Talpid Mutant. Front Cell Dev Biol. 2016;4:149 pubmed 出版商
  • 免疫印迹; 人类; 1:500; 图 4b
Coffman L, Choi Y, McLean K, Allen B, di Magliano M, Buckanovich R. Human carcinoma-associated mesenchymal stem cells promote ovarian cancer chemotherapy resistance via a BMP4/HH signaling loop. Oncotarget. 2016;7:6916-32 pubmed 出版商
  • 免疫细胞化学; 小鼠; 1:10; 图 3
Alfaro A, Roberts B, Kwong L, Bijlsma M, Roelink H. Ptch2 mediates the Shh response in Ptch1-/- cells. Development. 2014;141:3331-9 pubmed 出版商
  • 免疫组化-石蜡切片; Gallotia galloti; 1:2
Romero Alemán M, Monzon Mayor M, Santos E, Lang D, Yanes C. Neuronal and glial differentiation during lizard (Gallotia galloti) visual system ontogeny. J Comp Neurol. 2012;520:2163-84 pubmed 出版商
Yang C, Li X, Li Q, Li H, Qiao L, Guo Z, et al. Sonic Hedgehog Regulation of the Neural Precursor Cell Fate During Chicken Optic Tectum Development. J Mol Neurosci. 2018;64:287-299 pubmed 出版商
Avilés E, Stoeckli E. Canonical wnt signaling is required for commissural axon guidance. Dev Neurobiol. 2016;76:190-208 pubmed 出版商
Furmanski A, Saldana J, Ono M, Sahni H, Paschalidis N, D Acquisto F, et al. Tissue-derived hedgehog proteins modulate Th differentiation and disease. J Immunol. 2013;190:2641-9 pubmed 出版商
Voronova A, Coyne E, Al Madhoun A, Fair J, Bosiljcic N, St Louis C, et al. Hedgehog signaling regulates MyoD expression and activity. J Biol Chem. 2013;288:4389-404 pubmed 出版商
Boije H, Harun Or Rashid M, Lee Y, Imsland F, Bruneau N, Vieaud A, et al. Sonic Hedgehog-signalling patterns the developing chicken comb as revealed by exploration of the pea-comb mutation. PLoS ONE. 2012;7:e50890 pubmed 出版商
Wang W, Lian N, Ma Y, Li L, Gallant R, Elefteriou F, et al. Chondrocytic Atf4 regulates osteoblast differentiation and function via Ihh. Development. 2012;139:601-11 pubmed 出版商
Gerhart J, Scheinfeld V, Milito T, Pfautz J, Neely C, Fisher Vance D, et al. Myo/Nog cell regulation of bone morphogenetic protein signaling in the blastocyst is essential for normal morphogenesis and striated muscle lineage specification. Dev Biol. 2011;359:12-25 pubmed 出版商
Wilson N, Stoeckli E. Cell type specific, traceable gene silencing for functional gene analysis during vertebrate neural development. Nucleic Acids Res. 2011;39:e133 pubmed 出版商
Ohlig S, Farshi P, Pickhinke U, van den Boom J, Höing S, Jakuschev S, et al. Sonic hedgehog shedding results in functional activation of the solubilized protein. Dev Cell. 2011;20:764-74 pubmed 出版商
Farshi P, Ohlig S, Pickhinke U, Höing S, Jochmann K, Lawrence R, et al. Dual roles of the Cardin-Weintraub motif in multimeric Sonic hedgehog. J Biol Chem. 2011;286:23608-19 pubmed 出版商
Cho S, Kwak S, Woolley T, Lee M, Kim E, Baker R, et al. Interactions between Shh, Sostdc1 and Wnt signaling and a new feedback loop for spatial patterning of the teeth. Development. 2011;138:1807-16 pubmed 出版商
Nagy N, Oláh I. Experimental evidence for the ectodermal origin of the epithelial anlage of the chicken bursa of Fabricius. Development. 2010;137:3019-23 pubmed 出版商
Maun H, Wen X, Lingel A, De Sauvage F, Lazarus R, Scales S, et al. Hedgehog pathway antagonist 5E1 binds hedgehog at the pseudo-active site. J Biol Chem. 2010;285:26570-80 pubmed 出版商
Favaro R, Valotta M, Ferri A, Latorre E, Mariani J, Giachino C, et al. Hippocampal development and neural stem cell maintenance require Sox2-dependent regulation of Shh. Nat Neurosci. 2009;12:1248-56 pubmed 出版商
Kobune M, Takimoto R, Murase K, Iyama S, Sato T, Kikuchi S, et al. Drug resistance is dramatically restored by hedgehog inhibitors in CD34+ leukemic cells. Cancer Sci. 2009;100:948-55 pubmed 出版商
Ohyama K, Das R, Placzek M. Temporal progression of hypothalamic patterning by a dual action of BMP. Development. 2008;135:3325-31 pubmed 出版商
Yauch R, Gould S, Scales S, Tang T, Tian H, Ahn C, et al. A paracrine requirement for hedgehog signalling in cancer. Nature. 2008;455:406-10 pubmed 出版商
Reeber S, Sakai N, Nakada Y, Dumas J, Dobrenis K, Johnson J, et al. Manipulating Robo expression in vivo perturbs commissural axon pathfinding in the chick spinal cord. J Neurosci. 2008;28:8698-708 pubmed 出版商
Angot E, Loulier K, Nguyen Ba Charvet K, Gadeau A, Ruat M, Traiffort E. Chemoattractive activity of sonic hedgehog in the adult subventricular zone modulates the number of neural precursors reaching the olfactory bulb. Stem Cells. 2008;26:2311-20 pubmed 出版商
Zavros Y, Orr M, Xiao C, Malinowska D. Sonic hedgehog is associated with H+-K+-ATPase-containing membranes in gastric parietal cells and secreted with histamine stimulation. Am J Physiol Gastrointest Liver Physiol. 2008;295:G99-G111 pubmed 出版商
Peacock C, Wang Q, Gesell G, Corcoran Schwartz I, Jones E, Kim J, et al. Hedgehog signaling maintains a tumor stem cell compartment in multiple myeloma. Proc Natl Acad Sci U S A. 2007;104:4048-53 pubmed
Chan V, Chau S, Tian L, Chen Y, Kwong S, Quackenbush J, et al. Sonic hedgehog promotes CD4+ T lymphocyte proliferation and modulates the expression of a subset of CD28-targeted genes. Int Immunol. 2006;18:1627-36 pubmed
Hochman E, Castiel A, Jacob Hirsch J, Amariglio N, Izraeli S. Molecular pathways regulating pro-migratory effects of Hedgehog signaling. J Biol Chem. 2006;281:33860-70 pubmed
Riobo N, Lu K, Ai X, Haines G, Emerson C. Phosphoinositide 3-kinase and Akt are essential for Sonic Hedgehog signaling. Proc Natl Acad Sci U S A. 2006;103:4505-10 pubmed
Yu L, Gu S, Alappat S, Song Y, Yan M, Zhang X, et al. Shox2-deficient mice exhibit a rare type of incomplete clefting of the secondary palate. Development. 2005;132:4397-406 pubmed
Zeltser L. Shh-dependent formation of the ZLI is opposed by signals from the dorsal diencephalon. Development. 2005;132:2023-33 pubmed
Bok J, Bronner Fraser M, Wu D. Role of the hindbrain in dorsoventral but not anteroposterior axial specification of the inner ear. Development. 2005;132:2115-24 pubmed
Madison B, Braunstein K, Kuizon E, Portman K, Qiao X, Gumucio D. Epithelial hedgehog signals pattern the intestinal crypt-villus axis. Development. 2005;132:279-89 pubmed
Ju M, Aroca P, Luo J, Puelles L, Redies C. Molecular profiling indicates avian branchiomotor nuclei invade the hindbrain alar plate. Neuroscience. 2004;128:785-96 pubmed
Akazawa C, Tsuzuki H, Nakamura Y, Sasaki Y, Ohsaki K, Nakamura S, et al. The upregulated expression of sonic hedgehog in motor neurons after rat facial nerve axotomy. J Neurosci. 2004;24:7923-30 pubmed
Sanchez P, Hernandez A, Stecca B, Kahler A, DeGueme A, Barrett A, et al. Inhibition of prostate cancer proliferation by interference with SONIC HEDGEHOG-GLI1 signaling. Proc Natl Acad Sci U S A. 2004;101:12561-6 pubmed
Kobune M, Ito Y, Kawano Y, Sasaki K, Uchida H, Nakamura K, et al. Indian hedgehog gene transfer augments hematopoietic support of human stromal cells including NOD/SCID-beta2m-/- repopulating cells. Blood. 2004;104:1002-9 pubmed
Berman D, Karhadkar S, Maitra A, Montes De Oca R, Gerstenblith M, Briggs K, et al. Widespread requirement for Hedgehog ligand stimulation in growth of digestive tract tumours. Nature. 2003;425:846-51 pubmed
Zeng X, Goetz J, Suber L, Scott W, Schreiner C, Robbins D. A freely diffusible form of Sonic hedgehog mediates long-range signalling. Nature. 2001;411:716-20 pubmed
Zhang X, Yang X. Temporal and spatial effects of Sonic hedgehog signaling in chick eye morphogenesis. Dev Biol. 2001;233:271-90 pubmed
Outram S, Varas A, Pepicelli C, Crompton T. Hedgehog signaling regulates differentiation from double-negative to double-positive thymocyte. Immunity. 2000;13:187-97 pubmed
Wallace V, Raff M. A role for Sonic hedgehog in axon-to-astrocyte signalling in the rodent optic nerve. Development. 1999;126:2901-9 pubmed
Wallace V. Purkinje-cell-derived Sonic hedgehog regulates granule neuron precursor cell proliferation in the developing mouse cerebellum. Curr Biol. 1999;9:445-8 pubmed
Ericson J, Morton S, Kawakami A, Roelink H, Jessell T. Two critical periods of Sonic Hedgehog signaling required for the specification of motor neuron identity. Cell. 1996;87:661-73 pubmed
Mittelman A, Bertram J, Henry D, Snyder H, Messerschmidt G, Ciavarella D, et al. Treatment of patients with HIV thrombocytopenia and hemolytic uremic syndrome with protein A (Prosorba column) immunoadsorption. Semin Hematol. 1989;26:15-8 pubmed
产品信息
内部号码 :
2410
名称 :
5E1
Depositor Name :
Jessell, T.M. / Brenner-Morton, S.
Depositor Institution :
HHMI/Columbia University
Date Deposited :
12/3/96
Allow Hybridoma Distribution :
Yes
Cells Available (legacy) :
Yes
抗原 :
sonic hedgehog
抗原物种 :
大鼠
宿主物种 :
小鼠
抗体亚型 :
MIgG1
Isotype for catalog (legacy) :
IgG1
Positive Tested Species Reactivity :
鸡, 人类, 蜥蜴, 小鼠, 鹌鹑, 兔, 大鼠
Species Tested (legacy) :
chick, rat, mouse, human
Initial Publication Pubmed ID :
8929535
Depositor Notes (Special Instructions) :
Ligand for the Patched receptor
Collections :
Cell signaling,Extracellular matrix proteins,Human,Neurodevelopment
搜索关键词 :
Jessell, sonic hedgehog, mouse, brenner, SHH-N, Monoclonal, rat, MIgG1, Chicken, Rat, Mouse, Human,Quail, Rabbit, AB_2188307
抗原分子量 :
20 kDa SHH-N protein
基因 :
Shh
Alternate Gene Name(s) :
sonic hedgehog
UniProt数据库编号 :
Q63673
抗体登记处号码 :
AB_2188307
Synonyms (Alternate Clone Names) :
m5E1, 甲SHH
免疫原 :
合成肽
免疫原序列 :
N-terminal SSH fragment
克隆性 :
单克隆
Myeloma Strain :
NS1
表位定位 :
Yes
Epitope Location or Sequence :
氨基酸。 157-178
表位定位Pubmed登录号 :
20504762 21572042 21664575
Recommended Applications :
FACS,Function Blocking,Immunofluorescence,Immunohistochemistry,Western Blot
Immunoblotting (legacy) :
Yes
免疫组化PubMed IDs :
15464286 20702559 20504762 21824915 15590741 18753371 19734891
免疫荧光PubMed IDs :
21884693 18483183 15356205 16537363 18787065 18787065 29285739 29285739 27093082
免疫印迹PubMed IDs :
16943197 15105288
免疫沉淀PubMed IDs :
28859094
Function Blocking Pubmed IDs :
8929535 10981962 11336495 15314219 23266826 20504762 10357934 10226030 14520411 18617686 18754008 11395778 2658095 21664575 21572042 21447550 15788455 16943197 17005629 22190639 19245435 17360475 15788458 15105288 16141225 23408837
TRAP PubMed IDs :
20504762 18483183
Depositor Growth Medium :
20% fetal bovine serum in Iscove's DMEM with 1x pen/strep
其他信息 :
Also recognizes IHH (Indian Hedghog) PMID: 8662546 . The functionally blocked receptor is ptc. Does not bind to ShhC25S protein. For experiments involving live cells and tissue, supernatant of 5E1 is available without the antimicrobial ProClin. Contact us at 319-335-3826 or dshb@uiowa.edu for special ordering instructions.
DSHB增长培养基 :
Iscove's
References (legacy) :
Cell 87, 661-673.; Immunity 13, 187-197.; Dev. Biol. 233, 271-290.; Neuroscience 128, 785-796.; PNAS 101(34), 12561-12566.; Development 137, 3019-3023.; Dev. Biol. 359, 12-25.; J. Biol. Chem. 288(6), 4389-4404.
更多信息或购买 :
Developmental Studies Hybridoma Bank 产品网页
公司信息
Developmental Studies Hybridoma Bank
University of Iowa
dshb@uiowa.edu
http://dshb.biology.uiowa.edu
公司总部: 美国
Developmental Studies Hybridoma Bank (DSHB) 是NIH于1996年创办的国家机构，为生命科学研究领域及诊断领域，提供高质量的抗体等产品。