产品简要
公司名称 :
Developmental Studies Hybridoma Bank
产品类型 :
抗体
产品名称 :
整合素乙3(CD61), 人类
目录 :
7H2
克隆性 :
单克隆
宿主 :
小鼠
共轭标签 :
未共轭
克隆名称 :
7H2
反应物种 :
人类
应用 :
免疫印迹, 免疫组化, 免疫细胞化学, 免疫沉淀, 流式细胞仪
文章摘录数: 14
参考文献
Cheng M, Li J, Negri A, Coller B. Swing-out of the ?3 hybrid domain is required for ?IIb?3 priming and normal cytoskeletal reorganization, but not adhesion to immobilized fibrinogen. PLoS ONE. 2013;8:e81609 pubmed 出版商
Choi W, Rice W, Stokes D, Coller B. Three-dimensional reconstruction of intact human integrin ?IIb?3: new implications for activation-dependent ligand binding. Blood. 2013;122:4165-71 pubmed 出版商
Murcia M, Jirouskova M, Li J, Coller B, Filizola M. Functional and computational studies of the ligand-associated metal binding site of beta3 integrins. Proteins. 2008;71:1779-91 pubmed 出版商
Blue R, Murcia M, Karan C, Jirouskova M, Coller B. Application of high-throughput screening to identify a novel alphaIIb-specific small- molecule inhibitor of alphaIIbbeta3-mediated platelet interaction with fibrinogen. Blood. 2008;111:1248-56 pubmed
Jirouskova M, Jaiswal J, Coller B. Ligand density dramatically affects integrin alpha IIb beta 3-mediated platelet signaling and spreading. Blood. 2007;109:5260-9 pubmed
Mitchell W, Li J, Murcia M, Valentin N, Newman P, Coller B. Mapping early conformational changes in alphaIIb and beta3 during biogenesis reveals a potential mechanism for alphaIIbbeta3 adopting its bent conformation. Blood. 2007;109:3725-32 pubmed
Nelson E, Li J, Mitchell W, Chandy M, Srivastava A, Coller B. Three novel beta-propeller mutations causing Glanzmann thrombasthenia result in production of normally stable pro-alphaIIb, but variably impaired progression of pro-alphaIIbbeta3 from endoplasmic reticulum to Golgi. J Thromb Haemost. 2005;3:2773-83 pubmed
Mitchell W, Li J, Singh F, Michelson A, Bussel J, Coller B, et al. Two novel mutations in the alpha IIb calcium-binding domains identify hydrophobic regions essential for alpha IIbbeta 3 biogenesis. Blood. 2003;101:2268-76 pubmed
Basani R, French D, Vilaire G, Brown D, Chen F, Coller B, et al. A naturally occurring mutation near the amino terminus of alphaIIb defines a new region involved in ligand binding to alphaIIbbeta3. Blood. 2000;95:180-8 pubmed
Dangas G, Badimon J, Coller B, Fallon J, Sharma S, Hayes R, et al. Administration of abciximab during percutaneous coronary intervention reduces both ex vivo platelet thrombus formation and fibrin deposition: implications for a potential anticoagulant effect of abciximab. Arterioscler Thromb Vasc Biol. 1998;18:1342-9 pubmed
Grimaldi C, Chen F, Wu C, Weiss H, Coller B, French D. Glycoprotein IIb Leu214Pro mutation produces glanzmann thrombasthenia with both quantitative and qualitative abnormalities in GPIIb/IIIa. Blood. 1998;91:1562-71 pubmed
Grimaldi C, Chen F, Scudder L, Coller B, French D. A Cys374Tyr homozygous mutation of platelet glycoprotein IIIa (beta 3) in a Chinese patient with Glanzmann's thrombasthenia. Blood. 1996;88:1666-75 pubmed
Reverter J, Beguin S, Kessels H, Kumar R, Hemker H, Coller B. Inhibition of platelet-mediated, tissue factor-induced thrombin generation by the mouse/human chimeric 7E3 antibody. Potential implications for the effect of c7E3 Fab treatment on acute thrombosis and "clinical restenosis". J Clin Invest. 1996;98:863-74 pubmed
Kutok J, Coller B. Partial inhibition of platelet aggregation and fibrinogen binding by a murine monoclonal antibody to GPIIIa: requirement for antibody bivalency. Thromb Haemost. 1994;72:964-72 pubmed
产品信息
内部号码 :
8892
名称 :
7H2
Depositor Name :
Coller, B.S.
Depositor Institution :
Rockefeller University
Date Deposited :
5/2/13
Allow Hybridoma Distribution :
No
Cells Available (legacy) :
No
抗原 :
整合素乙3(CD61), 人类
抗原物种 :
人类
宿主物种 :
小鼠
抗体亚型 :
MIgG1
Isotype for catalog (legacy) :
IgG1
Positive Tested Species Reactivity :
人类, 灵长类动物
Species Tested (legacy) :
人类和灵长类动物
Initial Publication Pubmed ID :
7537915
Depositor Notes (Special Instructions) :
7H2 reacts with alphaIIbbeta3 complex on platelets and has variable effects on platelet function. The epitope recognized by 7H2 is located on the beta3 PSI domain (unpublished mutational analysis).
Collections :
CD antigens,Cell adhesion,Cell signaling,Human
搜索关键词 :
Coller, B.S., integrin beta-3 (CD61), human, Human, MIgG1, Human/Primate, ITGB3, GP3A, CD61, AB_2617394, monoclonal, Cell adhesion/Cluster Determinant antigens/Human/Cell signaling, Immunohistochemistry/Immunofluorescence/Western Blot/Immunoprecipitation/Function Blocking/FACS
抗原分子量 :
Predicted; 87 kDa; Apparent: 90 kDa
基因 :
ITGB3
Alternate Gene Name(s) :
GP3A, CD61
UniProt数据库编号 :
P05106
抗体登记处号码 :
AB_2617394
免疫原 :
人类血小板
免疫原序列 :
全长蛋白
克隆性 :
单克隆
Myeloma Strain :
X63-Ag8.653
表位定位 :
Yes
Epitope Location or Sequence :
整合素β3 PSI区域
表位定位Pubmed登录号 :
24136164
Recommended Applications :
FACS,Function Blocking,Immunofluorescence,Immunohistochemistry,Immunoprecipitation,Western Blot
Immunoblotting (legacy) :
yes
免疫组化PubMed IDs :
9714143
免疫荧光PubMed IDs :
24349096 17332246 17978171
免疫印迹PubMed IDs :
7537915 9473221 24349096 10607701 8781422 12424194
免疫沉淀PubMed IDs :
9473221 17209052 16359515
Function Blocking Pubmed IDs :
7537915 8698879
流式细胞仪PubMed IDs :
7537915 18175315
其他信息 :
7H2 recognizes free beta3 as weel as alphaVbeta3 and alphaIIbbeta3 and their pro-forms. In immunoblots, 7H2 doesn't recognize its target under reducing conditions.
DSHB增长培养基 :
Iscove's
References (legacy) :
Thromb. Haemost. 72(6), 964-972.; Blood 91(5), 1562-1571.
公司信息
Developmental Studies Hybridoma Bank
University of Iowa
dshb@uiowa.edu
http://dshb.biology.uiowa.edu
公司总部: 美国
Developmental Studies Hybridoma Bank (DSHB) 是NIH于1996年创办的国家机构,为生命科学研究领域及诊断领域,提供高质量的抗体等产品。