产品简要
公司名称 :
Developmental Studies Hybridoma Bank
产品类型 :
抗体
产品名称 :
骨结合素(SPARC)
目录 :
AON-1
克隆性 :
单克隆
宿主 :
小鼠
共轭标签 :
未共轭
克隆名称 :
AON-1
反应物种 :
人类, 大鼠, 狗, 牛
应用 :
免疫印迹, 酶联免疫吸附测定, 免疫组化, 免疫细胞化学, 免疫沉淀
更多信息或购买 :
Developmental Studies Hybridoma Bank 产品网页
文章摘录数: 15
出版应用/物种/样本/稀释参考文献
  • 免疫细胞化学; 人类; 1:5
Pautke C, Schieker M, Tischer T, Kolk A, Neth P, Mutschler W, et al. Characterization of osteosarcoma cell lines MG-63, Saos-2 and U-2 OS in comparison to human osteoblasts. Anticancer Res. 2004;24:3743-8 pubmed
Leblon C, Casey M, Fodor C, Zhang T, Zhang X, Jedlicka S. Correlation between in vitro expansion-related cell stiffening and differentiation potential of human mesenchymal stem cells. Differentiation. 2015;90:1-15 pubmed 出版商
Fedorovich N, Schuurman W, Wijnberg H, Prins H, van Weeren P, Malda J, et al. Biofabrication of osteochondral tissue equivalents by printing topologically defined, cell-laden hydrogel scaffolds. Tissue Eng Part C Methods. 2012;18:33-44 pubmed 出版商
Chen J, Yu Q, Wu B, Lin Z, Pavlos N, Xu J, et al. Autologous tenocyte therapy for experimental Achilles tendinopathy in a rabbit model. Tissue Eng Part A. 2011;17:2037-48 pubmed 出版商
Juhásová J, Juhás S, Klima J, Strnádel J, Holubova M, Motlik J. Osteogenic differentiation of miniature pig mesenchymal stem cells in 2D and 3D environment. Physiol Res. 2011;60:559-71 pubmed
Reding T, Wagner U, Silva A, Sun L, Bain M, Kim S, et al. Inflammation-dependent expression of SPARC during development of chronic pancreatitis in WBN/Kob rats and a microarray gene expression analysis. Physiol Genomics. 2009;38:196-204 pubmed 出版商
Koelling S, Kruegel J, Irmer M, Path J, Sadowski B, Miro X, et al. Migratory chondrogenic progenitor cells from repair tissue during the later stages of human osteoarthritis. Cell Stem Cell. 2009;4:324-35 pubmed 出版商
Cole A, Hall B. The nature and significance of invertebrate cartilages revisited: distribution and histology of cartilage and cartilage-like tissues within the Metazoa. Zoology (Jena). 2004;107:261-73 pubmed
Bampton E, Ma C, Tolkovsky A, Taylor J. Osteonectin is a Schwann cell-secreted factor that promotes retinal ganglion cell survival and process outgrowth. Eur J Neurosci. 2005;21:2611-23 pubmed
Lieb E, Vogel T, Milz S, Dauner M, Schulz M. Effects of transforming growth factor beta1 on bonelike tissue formation in three-dimensional cell culture. II: Osteoblastic differentiation. Tissue Eng. 2004;10:1414-25 pubmed
Loukopoulos P, O BRIEN T, Ghoddusi M, Mungall B, Robinson W. Characterisation of three novel canine osteosarcoma cell lines producing high levels of matrix metalloproteinases. Res Vet Sci. 2004;77:131-41 pubmed
Johansson C, Roser K, Bolind P, Donath K, Albrektsson T. Bone-tissue formation and integration of titanium implants: an evaluation with newly developed enzyme and immunohistochemical techniques. Clin Implant Dent Relat Res. 1999;1:33-40 pubmed
Roser K, Johansson C, Donath K, Albrektsson T. A new approach to demonstrate cellular activity in bone formation adjacent to implants. J Biomed Mater Res. 2000;51:280-91 pubmed
Bürgi Saville M, Gerber H, Peter H, Paulsson M, Aeschlimann D, Glaser C, et al. Expression patterns of extracellular matrix components in native and cultured normal human thyroid tissue and in human toxic adenoma tissue. Thyroid. 1997;7:347-56 pubmed
Bolander M, Robey P, Fisher L, Conn K, Prabhakar B, Termine J. Monoclonal antibodies against osteonectin show conservation of epitopes across species. Calcif Tissue Int. 1989;45:74-80 pubmed
产品信息
内部号码 :
761
名称 :
AON-1
Depositor Name :
Termine, J.D.
Depositor Institution :
Bone Research Branch, NIDR, NIH
Date Deposited :
7/13/90
Allow Hybridoma Distribution :
Yes
Cells Available (legacy) :
Yes
抗原 :
骨结合素(SPARC)
抗原物种 :
牛
宿主物种 :
小鼠
抗体亚型 :
MIgG1, kappa light chain
Isotype for catalog (legacy) :
IgG1, kappa light chain
Positive Tested Species Reactivity :
Acorn worm,Bovine,Canine,Human,Monkey,Pig,Rabbit,Rat
Species Tested (legacy) :
human, cow, monkey, pig, dog, rabbit, rat
Initial Publication Pubmed ID :
2476206
Depositor Notes (Special Instructions) :
The isotype of this monoclonal antibody was originally reported as IgG3 (PMID: 2476206). This antibody recognizes osteonectin (SPARC)-containing cartilages in invertebrate species. See PMID: 16351944.
Collections :
细胞粘附, 细胞外基质蛋白, 人类
搜索关键词 :
Termine, J.D., Osteonectin (SPARC), Bovine, MIgG1, kappa light chain, Human/Bovine/Monkey/Pig/canine/Rabbit/Rat/Acorn worm, SPARC, , AB_528421, Monoclonal , Human/Extracellular Matrix proteins, Western Blot/Immunoprecipitation/Immunohistochemistry/ELISA/Immunofluorescence/FFPE
抗原分子量 :
42 kDa
基因 :
SPARC
UniProt数据库编号 :
P13213
抗体登记处号码 :
AB_528421
免疫原 :
纯化牛骨结合素(SPARC)
免疫原序列 :
全长蛋白
克隆性 :
单克隆
Myeloma Strain :
x63-AG8.653
表位定位 :
No
Epitope Location or Sequence :
The epitope bound by AON-1 is cryptic and often requires tissue pretreatment for exposure. See PMID: 19435834 & 2476206.
Recommended Applications :
酶联免疫吸附测定, FFPE, 免疫荧光, 免疫组化, 免疫沉淀, 免疫印迹
Immunoblotting (legacy) :
Yes
免疫组化PubMed IDs :
10825228 11359309 2476206 15196903 19435834 21495863 21854293 15588401 31402634
免疫荧光PubMed IDs :
9226202 2476206 21401296 9226202 19341622 26381795
免疫印迹PubMed IDs :
2476206 15926910
免疫沉淀PubMed IDs :
2476206
酶联免疫吸附测定PubMed IDs :
2476206
FFPE PubMed IDs :
16351944 31402634
其他信息 :
RRID:AB_528421
DSHB增长培养基 :
Iscove's
References (legacy) :
Calcif. Tiss. Int. 45, 74-80.; Thyroid 7(3), 347-356.; Clin. Imp. Dent. Rel. Res. 1(1), 33-40.; J. Biomed. Mater. Res. 51, 280-291.; Res. Vet. Sci. 77, 131-141.; Eur. J. Neurosci. 21, 2611-2623.; Physiol. Genomics 38, 196-204.
更多信息或购买 :
Developmental Studies Hybridoma Bank 产品网页
公司信息
Developmental Studies Hybridoma Bank
University of Iowa
dshb@uiowa.edu
http://dshb.biology.uiowa.edu
公司总部: 美国
Developmental Studies Hybridoma Bank (DSHB) 是NIH于1996年创办的国家机构，为生命科学研究领域及诊断领域，提供高质量的抗体等产品。