产品简要
公司名称 :
Developmental Studies Hybridoma Bank
产品类型 :
抗体
产品名称 :
胰岛素(胰岛素原;C肽)
目录 :
GN-ID4
克隆性 :
单克隆
宿主 :
大鼠
共轭标签 :
未共轭
克隆名称 :
GN-ID4
反应物种 :
人类
应用 :
免疫组化, 免疫细胞化学, 免疫沉淀, 流式细胞仪, 流式细胞仪, 免疫组化-石蜡切片, 免疫组化-冰冻切片
文章摘录数: 24
出版应用/物种/样本/稀释 | 参考文献 |
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Brolén G, Heins N, Edsbagge J, Semb H. Signals from the embryonic mouse pancreas induce differentiation of human embryonic stem cells into insulin-producing beta-cell-like cells. Diabetes. 2005;54:2867-74 pubmed
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Itier J, Douhet P, Desbois P, Joshi R, Dandoy Dron F, Jami J, et al. Human insulin gene expression in transgenic mice: mutational analysis of the regulatory region. Differentiation. 1996;60:309-16 pubmed
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Douhet P, Destrade C, Bucchini D, Calas A. Expression of a human insulin transgene in cholinergic neurons of the mouse medial habenula. Biol Cell. 1995;85:137-46 pubmed
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Madsen O, Cohen R, Fitch F, Rubenstein A, Steiner D. The production and characterization of monoclonal antibodies specific for human proinsulin using a sensitive microdot assay procedure. Endocrinology. 1983;113:2135-44 pubmed
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Madsen O, Andersen L, Michelsen B, Owerbach D, Larsson L, Lernmark A, et al. Tissue-specific expression of transfected human insulin genes in pluripotent clonal rat insulinoma lines induced during passage in vivo. Proc Natl Acad Sci U S A. 1988;85:6652-6 pubmed
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Madsen O. Proinsulin-specific monoclonal antibodies. Immunocytochemical application as beta-cell markers and as probes for conversion. Diabetes. 1987;36:1203-11 pubmed
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Deltour L, Leduque P, Paldi A, Ripoche M, DuBois P, Jami J. Polyclonal origin of pancreatic islets in aggregation mouse chimaeras. Development. 1991;112:1115-21 pubmed
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Widagdo -, Sunardi -, Lokollo D, Margono S. Ocular angiostrongyliasis in Semarang, Central Java. Am J Trop Med Hyg. 1977;26:72-4 pubmed
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产品信息
内部号码 :
3373
名称 :
GN-ID4
Depositor Name :
Madsen, O.D.
Depositor Institution :
Hagedorn Research Institute
Date Deposited :
1/23/06
Allow Hybridoma Distribution :
No
Cells Available (legacy) :
No
抗原 :
胰岛素(胰岛素原;C肽)
抗原物种 :
人类
宿主物种 :
大鼠
抗体亚型 :
RIgG2a
Isotype for catalog (legacy) :
IgG2a
Positive Tested Species Reactivity :
Human,Monkey
Species Tested (legacy) :
human and monkey
Initial Publication Pubmed ID :
6196183
Depositor Notes (Special Instructions) :
This antibody recognizes the C-peptide (aa 33-63 of proinsulin) which separates insulin B chain (aa 1-30) from insulin A chain (aa 66-86) in the proinsulin protein (minus signal peptide sequence). Stains C-peptide in mature granules and proinsulin in immature granules of islet beta-cells. The antibody does not cross-react with rodent C-peptide/proinsulin.
Collections :
Cell markers,Cell signaling,Enzymes,Human,Stem cell
搜索关键词 :
Madsen, Ole D., insulin (proinsulin; C-peptide), Human, RIgG2a, Human/Monkey, INS, , AB_2255626, monoclonal
抗原分子量 :
Predicted: 9.4 kDa (proinsulin) and 3 kDa (C-peptide)
基因 :
INS
UniProt数据库编号 :
P01308
抗体登记处号码 :
AB_2255626
免疫原 :
Recombinant human proinsulin coupled to bovine thyroglobulin
免疫原序列 :
全长蛋白
克隆性 :
单克隆
Myeloma Strain :
SP2/0-Ag14
表位定位 :
Yes
Epitope Location or Sequence :
C-peptide (aa 40-45 and 57-63 of proinsulin)
表位定位Pubmed登录号 :
6196183
Recommended Applications :
流式细胞仪, 免疫荧光, 免疫组化, 免疫沉淀
免疫组化PubMed IDs :
2842785 2443408 1682130 842785
免疫荧光PubMed IDs :
23468018 26216140 29249360
免疫沉淀PubMed IDs :
6196183
流式细胞仪PubMed IDs :
26515645
PubMed IDs :
8855374 8785515
Depositor Growth Medium :
RPMI 1640 10% FCS, Pen/Strep & Fungizone
其他信息 :
This antibody stains unfixed frozen sections as well as frozen and paraffin sections of pancreas fixed with 4% PFA. Doesn't recognize bovine, canine or porcine proinsulin.
DSHB增长培养基 :
RPMI
References (legacy) :
Endocrinology 113(6), 2135-2144.; Diabetes 36(10), 1203-1211.; Proc. Natl. Acad. Sci. USA 85(18), 6652-6656.; Diabetes 54(10), 2867-2874.
公司信息
Developmental Studies Hybridoma Bank
Developmental Studies Hybridoma Bank (DSHB) 是NIH于1996年创办的国家机构,为生命科学研究领域及诊断领域,提供高质量的抗体等产品。