产品简要
公司名称 :
Developmental Studies Hybridoma Bank
产品类型 :
抗体
产品名称 :
胰岛素(胰岛素原;C肽)
目录 :
GN-ID4
克隆性 :
单克隆
宿主 :
大鼠
共轭标签 :
未共轭
克隆名称 :
GN-ID4
反应物种 :
人类
应用 :
免疫组化, 免疫细胞化学, 免疫沉淀, 流式细胞仪, 流式细胞仪, 免疫组化-石蜡切片, 免疫组化-冰冻切片
文章摘录数: 24
出版应用/物种/样本/稀释参考文献
  • 免疫组化; 人类; 1:100; 图 s3c
Low B, Lim C, Ding S, Tan Y, Ng N, Krishnan V, et al. Decreased GLUT2 and glucose uptake contribute to insulin secretion defects in MODY3/HNF1A hiPSC-derived mutant β cells. Nat Commun. 2021;12:3133 pubmed 出版商
  • 流式细胞仪; 人类; 图 7d
Yung T, Poon F, Liang M, Coquenlorge S, McGaugh E, Hui C, et al. Sufu- and Spop-mediated downregulation of Hedgehog signaling promotes beta cell differentiation through organ-specific niche signals. Nat Commun. 2019;10:4647 pubmed 出版商
  • 免疫组化-冰冻切片; 人类; 1:100; 图 e1a
  • 流式细胞仪; 人类; 1:100; 图 1a, s3b
Veres A, Faust A, Bushnell H, Engquist E, Kenty J, Harb G, et al. Charting cellular identity during human in vitro β-cell differentiation. Nature. 2019;569:368-373 pubmed 出版商
  • 免疫组化-石蜡切片; 人类; 图 7b
Sharon N, Chawla R, Mueller J, Vanderhooft J, Whitehorn L, Rosenthal B, et al. A Peninsular Structure Coordinates Asynchronous Differentiation with Morphogenesis to Generate Pancreatic Islets. Cell. 2019;176:790-804.e13 pubmed 出版商
  • 流式细胞仪; 人类; 1:500; 图 4b
  • 免疫细胞化学; 人类; 1:2000; 图 5b
Shi Z, Lee K, Yang D, Amin S, Verma N, Li Q, et al. Genome Editing in hPSCs Reveals GATA6 Haploinsufficiency and a Genetic Interaction with GATA4 in Human Pancreatic Development. Cell Stem Cell. 2017;20:675-688.e6 pubmed 出版商
  • 流式细胞仪; 人类; 1:50; 图 s20
  • 免疫细胞化学; 人类; 1:50; 图 3
Saxena P, Heng B, Bai P, Folcher M, Zulewski H, Fussenegger M. A programmable synthetic lineage-control network that differentiates human IPSCs into glucose-sensitive insulin-secreting beta-like cells. Nat Commun. 2016;7:11247 pubmed 出版商
  • 免疫组化-石蜡切片; 人类; 图 3
Vegas A, Veiseh O, Gürtler M, Millman J, Pagliuca F, Bader A, et al. Long-term glycemic control using polymer-encapsulated human stem cell-derived beta cells in immune-competent mice. Nat Med. 2016;22:306-11 pubmed 出版商
  • 流式细胞仪; 人类; 1:1000; 图 3
Korytnikov R, Nostro M. Generation of polyhormonal and multipotent pancreatic progenitor lineages from human pluripotent stem cells. Methods. 2016;101:56-64 pubmed 出版商
Leite N, Sintov E, Meissner T, Brehm M, Greiner D, Harlan D, et al. Modeling Type 1 Diabetes In Vitro Using Human Pluripotent Stem Cells. Cell Rep. 2020;32:107894 pubmed 出版商
Ramond C, Beydag Tasöz B, Azad A, van de Bunt M, Petersen M, Beer N, et al. Understanding human fetal pancreas development using subpopulation sorting, RNA sequencing and single-cell profiling. Development. 2018;145: pubmed 出版商
Wu X, Dao Thi V, Huang Y, Billerbeck E, Saha D, Hoffmann H, et al. Intrinsic Immunity Shapes Viral Resistance of Stem Cells. Cell. 2018;172:423-438.e25 pubmed 出版商
Dirice E, Walpita D, Vetere A, Meier B, Kahraman S, Hu J, et al. Inhibition of DYRK1A Stimulates Human β-Cell Proliferation. Diabetes. 2016;65:1660-71 pubmed 出版商
Braunstein I, Zach L, Allan S, Kalies K, Stanhill A. Proteasomal degradation of preemptive quality control (pQC) substrates is mediated by an AIRAPL-p97 complex. Mol Biol Cell. 2015;26:3719-27 pubmed 出版商
Asadi A, Bruin J, Kieffer T. Characterization of Antibodies to Products of Proinsulin Processing Using Immunofluorescence Staining of Pancreas in Multiple Species. J Histochem Cytochem. 2015;63:646-62 pubmed 出版商
Nostro M, Sarangi F, Yang C, Holland A, Elefanty A, Stanley E, et al. Efficient generation of NKX6-1+ pancreatic progenitors from multiple human pluripotent stem cell lines. Stem Cell Reports. 2015;4:591-604 pubmed 出版商
Sui L, Geens M, Sermon K, Bouwens L, Mfopou J. Role of BMP signaling in pancreatic progenitor differentiation from human embryonic stem cells. Stem Cell Rev. 2013;9:569-77 pubmed 出版商
Brolén G, Heins N, Edsbagge J, Semb H. Signals from the embryonic mouse pancreas induce differentiation of human embryonic stem cells into insulin-producing beta-cell-like cells. Diabetes. 2005;54:2867-74 pubmed
Itier J, Douhet P, Desbois P, Joshi R, Dandoy Dron F, Jami J, et al. Human insulin gene expression in transgenic mice: mutational analysis of the regulatory region. Differentiation. 1996;60:309-16 pubmed
Douhet P, Destrade C, Bucchini D, Calas A. Expression of a human insulin transgene in cholinergic neurons of the mouse medial habenula. Biol Cell. 1995;85:137-46 pubmed
Madsen O, Cohen R, Fitch F, Rubenstein A, Steiner D. The production and characterization of monoclonal antibodies specific for human proinsulin using a sensitive microdot assay procedure. Endocrinology. 1983;113:2135-44 pubmed
Madsen O, Andersen L, Michelsen B, Owerbach D, Larsson L, Lernmark A, et al. Tissue-specific expression of transfected human insulin genes in pluripotent clonal rat insulinoma lines induced during passage in vivo. Proc Natl Acad Sci U S A. 1988;85:6652-6 pubmed
Madsen O. Proinsulin-specific monoclonal antibodies. Immunocytochemical application as beta-cell markers and as probes for conversion. Diabetes. 1987;36:1203-11 pubmed
Deltour L, Leduque P, Paldi A, Ripoche M, DuBois P, Jami J. Polyclonal origin of pancreatic islets in aggregation mouse chimaeras. Development. 1991;112:1115-21 pubmed
Widagdo -, Sunardi -, Lokollo D, Margono S. Ocular angiostrongyliasis in Semarang, Central Java. Am J Trop Med Hyg. 1977;26:72-4 pubmed
产品信息
内部号码 :
3373
名称 :
GN-ID4
Depositor Name :
Madsen, O.D.
Depositor Institution :
Hagedorn Research Institute
Date Deposited :
1/23/06
Allow Hybridoma Distribution :
No
Cells Available (legacy) :
No
抗原 :
胰岛素(胰岛素原;C肽)
抗原物种 :
人类
宿主物种 :
大鼠
抗体亚型 :
RIgG2a
Isotype for catalog (legacy) :
IgG2a
Positive Tested Species Reactivity :
Human,Monkey
Species Tested (legacy) :
human and monkey
Initial Publication Pubmed ID :
6196183
Depositor Notes (Special Instructions) :
This antibody recognizes the C-peptide (aa 33-63 of proinsulin) which separates insulin B chain (aa 1-30) from insulin A chain (aa 66-86) in the proinsulin protein (minus signal peptide sequence). Stains C-peptide in mature granules and proinsulin in immature granules of islet beta-cells. The antibody does not cross-react with rodent C-peptide/proinsulin.
Collections :
Cell markers,Cell signaling,Enzymes,Human,Stem cell
搜索关键词 :
Madsen, Ole D., insulin (proinsulin; C-peptide), Human, RIgG2a, Human/Monkey, INS, , AB_2255626, monoclonal
抗原分子量 :
Predicted: 9.4 kDa (proinsulin) and 3 kDa (C-peptide)
基因 :
INS
UniProt数据库编号 :
P01308
抗体登记处号码 :
AB_2255626
免疫原 :
Recombinant human proinsulin coupled to bovine thyroglobulin
免疫原序列 :
全长蛋白
克隆性 :
单克隆
Myeloma Strain :
SP2/0-Ag14
表位定位 :
Yes
Epitope Location or Sequence :
C-peptide (aa 40-45 and 57-63 of proinsulin)
表位定位Pubmed登录号 :
6196183
Recommended Applications :
流式细胞仪, 免疫荧光, 免疫组化, 免疫沉淀
免疫组化PubMed IDs :
2842785 2443408 1682130 842785
免疫荧光PubMed IDs :
23468018 26216140 29249360
免疫沉淀PubMed IDs :
6196183
流式细胞仪PubMed IDs :
26515645
PubMed IDs :
8855374 8785515
Depositor Growth Medium :
RPMI 1640 10% FCS, Pen/Strep & Fungizone
其他信息 :
This antibody stains unfixed frozen sections as well as frozen and paraffin sections of pancreas fixed with 4% PFA. Doesn't recognize bovine, canine or porcine proinsulin.
DSHB增长培养基 :
RPMI
References (legacy) :
Endocrinology 113(6), 2135-2144.; Diabetes 36(10), 1203-1211.; Proc. Natl. Acad. Sci. USA 85(18), 6652-6656.; Diabetes 54(10), 2867-2874.
公司信息
Developmental Studies Hybridoma Bank
University of Iowa
dshb@uiowa.edu
http://dshb.biology.uiowa.edu
公司总部: 美国
Developmental Studies Hybridoma Bank (DSHB) 是NIH于1996年创办的国家机构,为生命科学研究领域及诊断领域,提供高质量的抗体等产品。