产品简要
公司名称 :
Developmental Studies Hybridoma Bank
产品类型 :
抗体
产品名称 :
胰岛素(胰岛素原)
目录 :
GS-9A8
克隆性 :
单克隆
宿主 :
小鼠
共轭标签 :
未共轭
克隆名称 :
GS-9A8
反应物种 :
人类, 小鼠, 大鼠, 狗
应用 :
免疫印迹, 免疫组化, 免疫细胞化学, 免疫沉淀, 免疫组化-冰冻切片
文章摘录数: 17
出版应用/物种/样本/稀释 | 参考文献 |
---|---|
| |
Madsen O, Frank B, Steiner D. Human proinsulin-specific antigenic determinants identified by monoclonal antibodies. Diabetes. 1984;33:1012-6 pubmed
| |
Madsen O, Cohen R, Fitch F, Rubenstein A, Steiner D. The production and characterization of monoclonal antibodies specific for human proinsulin using a sensitive microdot assay procedure. Endocrinology. 1983;113:2135-44 pubmed
| |
Orci L, Ravazzola M, Amherdt M, Madsen O, Vassalli J, Perrelet A. Direct identification of prohormone conversion site in insulin-secreting cells. Cell. 1985;42:671-81 pubmed
| |
Orci L, Ravazzola M, Storch M, Anderson R, Vassalli J, Perrelet A. Proteolytic maturation of insulin is a post-Golgi event which occurs in acidifying clathrin-coated secretory vesicles. Cell. 1987;49:865-8 pubmed
| |
Madsen O. Proinsulin-specific monoclonal antibodies. Immunocytochemical application as beta-cell markers and as probes for conversion. Diabetes. 1987;36:1203-11 pubmed
|
产品信息
内部号码 :
3378
名称 :
GS-9A8
Depositor Name :
Madsen, O.D.
Depositor Institution :
Hagedorn Research Institute
Date Deposited :
1/23/06
Allow Hybridoma Distribution :
No
Cells Available (legacy) :
No
抗原 :
胰岛素(胰岛素原)
抗原物种 :
人类
宿主物种 :
小鼠
抗体亚型 :
MIgG1
Isotype for catalog (legacy) :
IgG1
Positive Tested Species Reactivity :
Canine,Human,Monkey,Mouse,Rat
Species Tested (legacy) :
human and monkey
Initial Publication Pubmed ID :
6196183
Depositor Notes (Special Instructions) :
This antibody binds to the B-C junction of proinsulin of human and monkey and stains only intact, non-processed proinsulin found in the immature secretory granules of islet beta-cells. It does not cross-react to insulin nor C-peptide. Cross reaction to rodent proinsulins requires aldehyde fixation.
Collections :
Cell markers,Cell signaling,Enzymes,Human,Stem cell
搜索关键词 :
Madsen, O.D., insulin (proinsuline), Human, MIgG1, Human/Monkey/rat/mouse/canine, INS, , AB_532383, monoclonal, Cell signaling/Human/Stem cell/Cell markers, Immunohistochemistry/Immunofluorescence/Western Blot/Immunoprecipitation
抗原分子量 :
Predicted: 9.4 kDa
基因 :
INS
UniProt数据库编号 :
P01308
抗体登记处号码 :
AB_532383
免疫原 :
Recombinant human proinsulin coupled to bovine thyroglobulin
克隆性 :
单克隆
Myeloma Strain :
SP2/0-Ag14
表位定位 :
Yes
Epitope Location or Sequence :
B-C junction of proinsulin (aa 26-37)
表位定位Pubmed登录号 :
2443408 6207064
Recommended Applications :
免疫荧光, 免疫组化, 免疫沉淀, 免疫印迹
免疫组化PubMed IDs :
3555846 2443408 26801953 26323473
免疫荧光PubMed IDs :
3896518 2443408 27399229 26220792 26216140 26627735 22872759 28877460
免疫印迹PubMed IDs :
26220792
免疫沉淀PubMed IDs :
6196183
其他信息 :
Doesn't recognize bovine or porcine proinsulin.
DSHB增长培养基 :
RPMI
References (legacy) :
Endocrinology 113(6), 2135-2144.; Cell 42(2), 671-681.; Cell 49(6), 865-868.; Diabetes 36(10), 1203-1211.
公司信息
Developmental Studies Hybridoma Bank
Developmental Studies Hybridoma Bank (DSHB) 是NIH于1996年创办的国家机构,为生命科学研究领域及诊断领域,提供高质量的抗体等产品。